Amylin antibody | R10/99

Mouse anti Human Amylin

Product Type
Monoclonal Antibody
Clone
R10/99
Isotype
IgG1
Specificity
Amylin

Product Code Applications Pack Size List Price Your Price Qty
MCA1126
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF P 2 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human Amylin antibody, clone R10/99 recognizes the islet amyloid polypeptide (amylin). Mouse anti Human Amylin antibody, clone R10/99 is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
0.1M TRIS
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
A peptide CATQRLANFLV corresponding to residues 40-50 of human IAPP coupled to tuberculin.
Fusion Partners
Spleen cells from immunized a BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/100
Immunohistology - Paraffin 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Amylin antibody

  1. () The Human Protein Atlas: AB000352
    The Human Protein Atlas: AB000352
  2. Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
    The Open Pathology Journal. 2 (1): 86-95.
  3. Gong, W. et al. (2007) Amylin deposition in the kidney of patients with diabetic nephropathy.
    Kidney Int. 72 (2): 213-8.
  4. Bhattacharya, S. et al. (2007) Cloning and expression of human islet amyloid polypeptide in cultured cells.
    Biochem Biophys Res Commun. 356: 622-8.
  5. Gurlo, T. et al. (2010) Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway.
    Am J Pathol. 176: 861-9.
  6. Zhao, H. et al. (2010) Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition.
    Pancreas. 39: 843-6.
  7. Nakamura, S. et al. (2008) Transthyretin amyloidosis and two other aging-related amyloidoses in an aged vervet monkey.
    Vet Pathol. 45: 67-72.
  8. Zhao, H.L. et al. (2009) Amyloid oligomers in diabetic and nondiabetic human pancreas.
    Transl Res. 153: 24-32.
  9. View The Latest Product References
  10. Campbell-Thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
    J Vis Exp. 63: e4068.
  11. Zhang, X. et al. (2011) Conformation-dependent scFv antibodies specifically recognize the oligomers assembled from various amyloids and show colocalization of amyloid fibrils with oligomers in patients with amyloidoses.
    Biochim Biophys Acta. 1814 (12): 1703-12.
  12. Ramos-Vera, J.A. et al. (2016) Advanced Diagnostic Techniques
    In: Canine and Feline Cytology - E-Book: A Color Atlas and Interpretation Guide
  13. Lee, J. et al. (2013) Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Elife. 2: e00940.
  14. Zhang, X.X. et al. (2018) Human amylin induces CD4+Foxp3+ regulatory T cells in the protection from autoimmune diabetes.
    Immunol Res. 66 (1): 179-86.

Immunohistology - Paraffin

RRID
AB_2121635
UniProt
P10997
Entrez Gene
IAPP
GO Terms
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0007165 signal transduction
GO:0005179 hormone activity
GO:0005625 soluble fraction
GO:0007267 cell-cell signaling
GO:0031018 endocrine pancreas development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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