Amylin antibody | R10/99

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Anti Human Amylin Antibody, clone R10/99 thumbnail image 1

Anti Human Amylin Antibody, clone R10/99 thumbnail image 2

Anti Human Amylin Antibody, clone R10/99 thumbnail image 3

Anti Human Amylin Antibody, clone R10/99 gallery image 1 — Staining of paraffin embedded human pancreas with Mouse anti Human amylin Peptide antibody, clone R10/99 (**MCA1126**), showing labeling of Islet of Langerhans

Anti Human Amylin Antibody, clone R10/99 gallery image 2 — **Published customer image:**
Mouse anti amylin antibody, clone R10/99 (**MCA1126**) used to label IAPP expressing cells in cultured pancreatic cell spheres by immunofluorescence.
**Image caption:**
Induced insulin-secreting cells resemble functional β-cells.
(D) Representative confocal images of 4TFM spheres immunostained with indicated antibodies. Scale bar, 20 μm.

From: Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann JF, Miyazaki S, Miyazaki J, Szot GL, Bottino R, Kim SK.
Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Elife. 2013 Nov 19;2:e00940.

Anti Human Amylin Antibody, clone R10/99 gallery image 3 — Immunofluorescence staining of paraffin embedded human pancreas using Guinea Pig anti-Pig Insulin antibody (5330-0104G) (A) and Mouse anti-Human Amylin antibody (MCA1126) (B). Merged image showing co-localization of the two proteins (C). DAPI was used as nuclear counterstain (blue)

Mouse anti Human Amylin

Product Type: Monoclonal Antibody
Clone: R10/99
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1126T	C IF P			0.25 ml
MCA1126	C IF P			2 ml

Mouse anti Human Amylin antibody, clone R10/99 recognizes the islet amyloid polypeptide (amylin). Mouse anti Human Amylin antibody, clone R10/99 is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.

Product Details

Target Species

Human

Product Form

Tissue Culture Supernatant - liquid

Buffer Solution

0.1M TRIS

Preservative Stabilisers

Pack Size: 0.25 ml

0.09% Sodium Azide (NaN₃)

Pack Size: 2 ml

0.09%

Sodium Azide

Immunogen

A peptide CATQRLANFLV corresponding to residues 40-50 of human IAPP coupled to tuberculin

Fusion Partners

Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P10997 Related reagents
Entrez Gene: IAPP Related reagents
GO Terms: GO:0006915 apoptosis; GO:0005576 extracellular region; GO:0007165 signal transduction; GO:0005179 hormone activity; GO:0005625 soluble fraction; GO:0007267 cell-cell signaling; GO:0031018 endocrine pancreas development
Regulatory: For research purposes only

Applications of Amylin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Immunohistology - Frozen			1/100
Immunohistology - Paraffin			1/100

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Amylin antibody

Gong, W. et al. (2007) Amylin deposition in the kidney of patients with diabetic nephropathy.
Kidney Int. 72 (2): 213-8.
Bhattacharya, S. et al. (2007) Cloning and expression of human islet amyloid polypeptide in cultured cells.
Biochem Biophys Res Commun. 356: 622-8.
Gurlo, T. et al. (2010) Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway.
Am J Pathol. 176: 861-9.
Zhao, H. et al. (2010) Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition.
Pancreas. 39: 843-6.
Nakamura, S. et al. (2008) Transthyretin amyloidosis and two other aging-related amyloidoses in an aged vervet monkey.
Vet Pathol. 45: 67-72.
Zhao, H.L. et al. (2009) Amyloid oligomers in diabetic and nondiabetic human pancreas.
Transl Res. 153: 24-32.
Campbell-Thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
J Vis Exp. 63: e4068.
Zhang, X. et al. (2011) Conformation-dependent scFv antibodies specifically recognize the oligomers assembled from various amyloids and show colocalization of amyloid fibrils with oligomers in patients with amyloidoses.
Biochim Biophys Acta. 1814 (12): 1703-12.
Ramos-Vera, J.A. et al. (2016) Advanced Diagnostic Techniques
In: Canine and Feline Cytology - E-Book: A Color Atlas and Interpretation Guide
Lee, J. et al. (2013) Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Elife. 2: e00940.
Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
The Open Pathology Journal. 2 (1): 86-95.
Zhang, X.X. et al. (2017) Human amylin induces CD4+Foxp3+ regulatory T cells in the protection from autoimmune diabetes.
Immunol Res. Oct 05 [Epub ahead of print].

Request a different product with the Amylin specificity