Amylin antibody | R10/99
Mouse anti amylin antibody, clone R10/99 (MCA1126) used to label IAPP expressing cells in cultured pancreatic cell spheres by immunofluorescence.
Image caption:
Induced insulin-secreting cells resemble functional β-cells.
(D) Representative confocal images of 4TFM spheres immunostained with indicated antibodies. Scale bar, 20 μm.
From: Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann JF, Miyazaki S, Miyazaki J, Szot GL, Bottino R, Kim SK.
Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Elife. 2013 Nov 19;2:e00940.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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P IF ResetMouse anti Human Amylin
- Product Type
- Monoclonal Antibody
- Clone
- R10/99
- Isotype
- IgG1
- Specificity
- Amylin
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Human Amylin antibody, clone R10/99 recognizes the islet amyloid polypeptide (amylin). Mouse anti Human Amylin antibody, clone R10/99 is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid. |
- Target Species
- Human
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- 0.1M TRIS
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A peptide CATQRLANFLV corresponding to residues 40-50 of human IAPP coupled to tuberculin.
- Fusion Partners
- Spleen cells from immunized a BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
1/100 | |
| Immunohistology - Paraffin | |
1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
References for Amylin antibody
-
() The Human Protein Atlas: AB000352
The Human Protein Atlas: AB000352 -
Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
The Open Pathology Journal. 2 (1): 86-95. -
Gong, W. et al. (2007) Amylin deposition in the kidney of patients with diabetic nephropathy.
Kidney Int. 72 (2): 213-8. -
Bhattacharya, S. et al. (2007) Cloning and expression of human islet amyloid polypeptide in cultured cells.
Biochem Biophys Res Commun. 356: 622-8. -
Gurlo, T. et al. (2010) Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway.
Am J Pathol. 176: 861-9. -
Zhao, H. et al. (2010) Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition.
Pancreas. 39: 843-6. -
Nakamura, S. et al. (2008) Transthyretin amyloidosis and two other aging-related amyloidoses in an aged vervet monkey.
Vet Pathol. 45: 67-72. -
Zhao, H.L. et al. (2009) Amyloid oligomers in diabetic and nondiabetic human pancreas.
Transl Res. 153: 24-32.
View The Latest Product References
-
Campbell-Thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
J Vis Exp. 63: e4068. -
Zhang, X. et al. (2011) Conformation-dependent scFv antibodies specifically recognize the oligomers assembled from various amyloids and show colocalization of amyloid fibrils with oligomers in patients with amyloidoses.
Biochim Biophys Acta. 1814 (12): 1703-12. -
Ramos-Vera, J.A. et al. (2016) Advanced Diagnostic Techniques
In: Canine and Feline Cytology - E-Book: A Color Atlas and Interpretation Guide -
Lee, J. et al. (2013) Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Elife. 2: e00940. -
Zhang, X.X. et al. (2018) Human amylin induces CD4+Foxp3+ regulatory T cells in the protection from autoimmune diabetes.
Immunol Res. 66 (1): 179-86.
- RRID
- AB_2121635
- UniProt
- P10997
- Entrez Gene
- IAPP
- GO Terms
- GO:0006915 apoptosis
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0007267 cell-cell signaling
- GO:0031018 endocrine pancreas development
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