Amylin antibody | R10/99
Mouse anti Human Amylin antibody, clone R10/99 recognizes the islet amyloid polypeptide (amylin). Mouse anti Human Amylin antibody, clone R10/99 is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- 0.1M TRIS
- Preservative Stabilisers
- Pack Size: 0.25 ml0.09% Sodium Azide (NaN3)Pack Size: 2 ml
0.09% Sodium Azide
- A peptide CATQRLANFLV corresponding to residues 40-50 of human IAPP coupled to tuberculin
- Fusion Partners
- Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P10997 Related reagents
- Entrez Gene
- IAPP Related reagents
- GO Terms
- GO:0006915 apoptosis
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0007267 cell-cell signaling
- GO:0031018 endocrine pancreas development
- For research purposes only
Applications of Amylin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/100|
|Immunohistology - Paraffin||1/100|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Amylin antibody
Gong, W. et al. (2007) Amylin deposition in the kidney of patients with diabetic nephropathy.
Kidney Int. 72 (2): 213-8.
Bhattacharya, S. et al. (2007) Cloning and expression of human islet amyloid polypeptide in cultured cells.
Biochem Biophys Res Commun. 356: 622-8.
Gurlo, T. et al. (2010) Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway.
Am J Pathol. 176: 861-9.
Zhao, H. et al. (2010) Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition.
Pancreas. 39: 843-6.
Nakamura, S. et al. (2008) Transthyretin amyloidosis and two other aging-related amyloidoses in an aged vervet monkey.
Vet Pathol. 45: 67-72.
Zhao, H.L. et al. (2009) Amyloid oligomers in diabetic and nondiabetic human pancreas.
Transl Res. 153: 24-32.
Campbell-Thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
J Vis Exp. 63: e4068.
Zhang, X. et al. (2011) Conformation-dependent scFv antibodies specifically recognize the oligomers assembled from various amyloids and show colocalization of amyloid fibrils with oligomers in patients with amyloidoses.
Biochim Biophys Acta. 1814 (12): 1703-12.
Ramos-Vera, J.A. et al. (2016) Advanced Diagnostic Techniques
In: Canine and Feline Cytology - E-Book: A Color Atlas and Interpretation Guide
Lee, J. et al. (2013) Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Elife. 2: e00940.
Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
The Open Pathology Journal. 2 (1): 86-95.
Zhang, X.X. et al. (2017) Human amylin induces CD4+Foxp3+ regulatory T cells in the protection from autoimmune diabetes.
Immunol Res. Oct 05 [Epub ahead of print].