Mouse anti Human Amylin antibody, clone R10/99 recognizes the islet amyloid polypeptide (amylin). Mouse anti Human Amylin antibody, clone R10/99 is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- 0.1M TRIS
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- A peptide CATQRLANFLV corresponding to residues 40-50 of human IAPP coupled to tuberculin.
- Fusion Partners
- Spleen cells from immunized a BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
endocrine pancreas development
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Amylin antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for Amylin antibody
() The Human Protein Atlas: AB000352
The Human Protein Atlas: AB000352
Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
The Open Pathology Journal. 2 (1): 86-95.
Gong, W. et al. (2007) Amylin deposition in the kidney of patients with diabetic nephropathy.
Kidney Int. 72 (2): 213-8.
Bhattacharya, S. et al. (2007) Cloning and expression of human islet amyloid polypeptide in cultured cells.
Biochem Biophys Res Commun. 356: 622-8.
Gurlo, T. et al. (2010) Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway.
Am J Pathol. 176: 861-9.
Zhao, H. et al. (2010) Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition.
Pancreas. 39: 843-6.
Nakamura, S. et al. (2008) Transthyretin amyloidosis and two other aging-related amyloidoses in an aged vervet monkey.
Vet Pathol. 45: 67-72.
Zhao, H.L. et al. (2009) Amyloid oligomers in diabetic and nondiabetic human pancreas.
Transl Res. 153: 24-32.
Campbell-Thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
J Vis Exp. 63: e4068.
Zhang, X. et al. (2011) Conformation-dependent scFv antibodies specifically recognize the oligomers assembled from various amyloids and show colocalization of amyloid fibrils with oligomers in patients with amyloidoses.
Biochim Biophys Acta. 1814 (12): 1703-12.
Ramos-Vera, J.A. et al. (2016) Advanced Diagnostic Techniques
In: Canine and Feline Cytology - E-Book: A Color Atlas and Interpretation Guide
Lee, J. et al. (2013) Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Elife. 2: e00940.
Zhang, X.X. et al. (2018) Human amylin induces CD4+Foxp3+ regulatory T cells in the protection from autoimmune diabetes.
Immunol Res. 66 (1): 179-86.
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