AMH antibody | 5/6
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|Mouse anti Human AMH, clone 5/6 recognizes human anti-mullerian hormone (AMH), originally classified as a foetal testicular hormone that inhibits Mullerian duct development. AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric ~140 kDa disulphide linked precursor that is cleaved to release the mature ~30 kDa homodimer.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Sheep Squirrel monkey Baboon
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Concentrated Tissue Culture Supernatant containing 0.1M Tris/HCl pH7.4 and 5-10% foetal calf serum.
- Preservative Stabilisers
- 0.1% sodium azide (NaN3)
- Synthetic peptide derived from human AMH (VPTAYAGKLLISLSEERISAHHVPNMVATEC)
- Fusion Partners
- Spleen cells from immunised T/O outbred mice were fused with cells of the SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/20||1/40|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- human ovary
References for AMH antibody
Van Saen, D. et al. (2010) Meiotic activity in orthotopic xenografts derived from human postpubertal testicular tissue.
Hum Reprod. 26: 282-93.
Gruijters, M.J et al. (2003) Anti-Müllerian hormone and its role in ovarian function.
Mol Cell Endocrinol. 211 (1-2): 85-90.
Weenen, C. et al. (2004) Anti-Mullerian hormone expression pattern in the human ovary: potential implications for initial and cyclic follicle recruitment.
Mol Hum Reprod10: 77-83.
Papanastasopoulos, P. et al. (2009) A case of complete androgen insensitivity syndrome presenting with incarcerated inguinal hernia: an immunohistochemical study.
Fertil Steril. 92: 1169.e11-4.
Campbell, B.K. (2009) The endocrine and local control of ovarian follicle development in the ewe
Anim. Reprod. 6:159-71
Walker, M.L. et al. (2009) Ovarian aging in squirrel monkeys (Saimiri sciureus).
Reproduction. 138: 793-9.
Sobinoff, A.P. et al. (2011) Understanding the villain: DMBA induced pre-antral ovotoxicity involves selective follicular destruction and primordial follicle activation through PI3K/Akt and mTOR signalling.
Toxicol Sci. 123: 563-75.
Van Saen, D. et al. (2011) Can pubertal boys with Klinefelter syndrome benefit from spermatogonial stem cell banking?
Hum Reprod. 27: 323-30.
View The Latest Product References
David, A. et al. (2012) Effect of cryopreservation and transplantation on the expression of kit ligand and anti-Müllerian hormone in human ovarian tissue
Hum Reprod. 27: 1088-95.
Kevenaar, M.E. et al. (2006) Serum anti-mullerian hormone levels reflect the size of the primordial follicle pool in mice.
Endocrinology. 147: 3228-34.
Campbell, B.K. et al. (2012) The role of anti-Müllerian hormone (AMH) during follicle development in a monovulatory species (sheep).
Endocrinology. 153: 4533-43.
Amorim, C.A. et al. (2013) Successful vitrification and autografting of baboon (Papio anubis) ovarian tissue.
Hum Reprod. 28: 2146-56.
Parlakgumus, H.A. et al. (2015) GNRH agonists and antagonists in rescue for cyclophosphamide-induced ovarian damage: friend or foe?
Arch Gynecol Obstet. 291 (6): 1403-10.
ThemmenA, P.N. et al. (2016) The use of anti-Müllerian hormone as diagnostic for gonadectomy status in dogs.
Theriogenology. 86 (6): 1467-74.
Saatcioglu, H.D. et al. (2016) Control of Oocyte Reawakening by Kit.
PLoS Genet. 12 (8): e1006215.
Calvopina, J.H. et al. (2015) The Aorta-Gonad-Mesonephros Organ Culture Recapitulates 5hmC Reorganization and Replication-Dependent and Independent Loss of DNA Methylation in the Germline.
Stem Cells Dev. 24 (13): 1536-45.
Camlin, N.J. et al. (2016) Maternal Smoke Exposure Impairs the Long-Term Fertility of Female Offspring in a Murine Model.
Biol Reprod. 94 (2): 39.
Ohta, K. et al. (2012) Male differentiation of germ cells induced by embryonic age-specific Sertoli cells in mice.
Biol Reprod. 86 (4): 112.
Bazzano, M.V. et al. (2015) Obesity induced by cafeteria diet disrupts fertility in the rat by affecting multiple ovarian targets.
Reprod Biomed Online. 31 (5): 655-67.
Díaz, P.U. et al. (2018) Altered Expression of Anti-Müllerian Hormone during the Early Stage of Bovine Persistent Ovarian Follicles.
J Comp Pathol. 158: 22-31.
Cacciottola, L. et al. (2020) Long-Term Advantages of Ovarian Reserve Maintenance and Follicle Development Using Adipose Tissue-Derived Stem Cells in Ovarian Tissue Transplantation.
J Clin Med. 9 (9)Sep 15 [Epub ahead of print].
Lv, X. et al. (2020) Effects of Single Transplantation and Multiple Transplantation of Human Umbilical Cord Mesenchymal Stem Cells on the Recovery of Ovarian Function in the Treatment of Premature Ovarian Failure in Mice
Res Sq; Dec 01 [Preprint, Epub ahead of print].
Wang, F. et al. (2013) Human amniotic epithelial cells can differentiate into granulosa cells and restore folliculogenesis in a mouse model of chemotherapy-induced premature ovarian failure.
Stem Cell Res Ther. 4 (5): 124.
de Michele, F. et al. (2018) In vitro. formation of the blood-testis barrier during long-term organotypic culture of human prepubertal tissue: comparison with a large cohort of pre/peripubertal boys.
Mol Hum Reprod. 24 (5): 271-82.
- anti Mullerian Hormone
- Entrez Gene
- GO Terms
- GO:0001880 Mullerian duct regression
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0007267 cell-cell signaling
- GO:0007506 gonadal mesoderm development
- GO:0007530 sex determination
- GO:0008083 growth factor activity
- GO:0030154 cell differentiation
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