Alpha A Crystallin antibody | c9F2

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Mouse anti Human Alpha A Crystallin

Product Type
Monoclonal Antibody
Clone
c9F2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2696 E WB 0.1 mg
Mouse anti Human Alpha A Crystallin antibody, clone c9F2 recognizes alpha A crystallin, a 20 kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB4, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index.

Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
10% Glycerol
Immunogen
E. coli-derived recombinant protein corresponding to full-length (aa1-173) human crystallin alpha A.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02489 Related reagents
Entrez Gene
CRYAA Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005212 structural constituent of eye lens
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0007601 visual perception
GO:0009408 response to heat
GO:0032387 negative regulation of intracellular transport
GO:0051260 protein homooligomerization
Regulatory
For research purposes only

Applications of Alpha A Crystallin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Andley UP et al. (1996) Cloning, expression, and chaperone-like activity of human alphaA-crystallin.
    J Biol Chem. 271 (50): 31973-80.