Alpha A Crystallin antibody | c9F2
Mouse anti Human Alpha A Crystallin antibody, clone c9F2 recognizes alpha A crystallin, a 20 kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB4, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index.
Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus.
Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
10% Glycerol - Immunogen
- E. coli-derived recombinant protein corresponding to full-length (aa1-173) human crystallin alpha A.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02489
- Entrez Gene
- CRYAA
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005212 structural constituent of eye lens
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006916 anti-apoptosis
- GO:0007601 visual perception
- GO:0009408 response to heat
- GO:0032387 negative regulation of intracellular transport
- GO:0051260 protein homooligomerization
- Regulatory
- For research purposes only
Applications of Alpha A Crystallin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | 1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
Further Reading
-
Andley UP et al. (1996) Cloning, expression, and chaperone-like activity of human alphaA-crystallin.
J Biol Chem. 271 (50): 31973-80.
