AKT1 antibody | 6F11
Mouse anti Human AKT1 antibody, clone 6F11 recognizes human RAC-alpha serine/threonine-protein kinase, also known as AKT1, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt or RAC-PK-alpha. AKT1 is a 480 amino acid ~54 kDa serine/threonine-protein kinase involved in many metabolic, proliferative, growth and angiogenic processes.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1-481 of human AKT1.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P31749
- Entrez Gene
- AKT1
- GO Terms
- GO:0005886 plasma membrane
- GO:0000060 protein import into nucleus, translocation
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
- GO:0005654 nucleoplasm
- GO:0005978 glycogen biosynthetic process
- GO:0006417 regulation of translation
- GO:0006469 negative regulation of protein kinase activity
- GO:0006809 nitric oxide biosynthetic process
- GO:0006924 activation-induced cell death of T cells
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0042802 identical protein binding
- GO:0009408 response to heat
- GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
- GO:0010975 regulation of neuron projection development
- GO:0016071 mRNA metabolic process
- GO:0046326 positive regulation of glucose import
- GO:0046889 positive regulation of lipid biosynthetic process
- GO:0019899 enzyme binding
- GO:0018105 peptidyl-serine phosphorylation
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030168 platelet activation
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030307 positive regulation of cell growth
- GO:0031018 endocrine pancreas development
- GO:0031295 T cell costimulation
- GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
- GO:0031999 negative regulation of fatty acid beta-oxidation
- GO:0034405 response to fluid shear stress
- GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045600 positive regulation of fat cell differentiation
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0046777 protein autophosphorylation
- GO:0048009 insulin-like growth factor receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051000 positive regulation of nitric-oxide synthase activity
- GO:0070141 response to UV-A
- GO:0090004 positive regulation of establishment of protein localization in plasma membrane
- Regulatory
- For research purposes only
Applications of AKT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml | |
| Western Blotting | 0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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