Mouse anti Human AKT1 antibody, clone 6F11 recognizes human RAC-alpha serine/threonine-protein kinase, also known as AKT1, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt or RAC-PK-alpha. AKT1 is a 480 amino acid ~54 kDa serine/threonine-protein kinase involved in many metabolic, proliferative, growth and angiogenic processes.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 1-481 of human AKT1.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein import into nucleus, translocation
protein serine/threonine kinase activity
glycogen biosynthetic process
regulation of translation
negative regulation of protein kinase activity
nitric oxide biosynthetic process
activation-induced cell death of T cells
G-protein coupled receptor protein signaling pathway
insulin receptor signaling pathway
induction of apoptosis by intracellular signals
activation of pro-apoptotic gene products
identical protein binding
response to heat
negative regulation of plasma membrane long-chain fatty acid transport
regulation of neuron projection development
mRNA metabolic process
positive regulation of glucose import
positive regulation of lipid biosynthetic process
nitric-oxide synthase regulator activity
positive regulation of cell growth
endocrine pancreas development
T cell costimulation
positive regulation of cyclin-dependent protein kinase activity involved in G1/S
negative regulation of fatty acid beta-oxidation
response to fluid shear stress
positive regulation of nitric oxide biosynthetic process
positive regulation of fat cell differentiation
positive regulation of glycogen biosynthetic process
insulin-like growth factor receptor signaling pathway
nerve growth factor receptor signaling pathway
positive regulation of nitric-oxide synthase activity
response to UV-A
positive regulation of establishment of protein localization in plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of AKT1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available