Aggrecan antibody | 7E1
Mouse anti Human aggrecan antibody, clone 7E1 (MCA1451G) used for the detection of aggrecan expressing cells in murine micromass cultures by immunocytochemistry.
Image caption:
Regulation of Extracellular matrix (ECM) composition by Bone morphogenetic protein (BMP) family growth factors during chondrogenesis. Mouse micromass cultures without treatment (A–E), Gdf-5 treatment (F–J), and Bmp-7 treatment (K–O); Alcian blue stain (A–K) showed cartilage nodules, Immunohistochemical stain for type 1 collagen (A, F, K), type II collagen (C, H, M), aggrecan (D, I, N), and fibronectin (E, J, O). Statistical analysis for histochemical and immunohistochemical data, (P) = percentage of positive area for Alcian blue, type I collagen, type II collagen, aggrecan, and fibronectin stains in micromasses with different treatments; mean values are shown with Standard deviations (SD) (n = three independent experiments), One-way Analysis of variance (ANOVA). ***P <0.0001; **p <0.001; *p <0.05). Gdf-5 mainly induces aggrecan expression, type II collagen, and fibronectin; aggrecan is an articular cartilage marker; in contrast, Bmp-7 induce type I collagen, an extracellular component of hypertrophic cartilage and bone.
From: Garciadiego-Cázares D, Aguirre-Sánchez HI, Abarca-Buis RF, Kouri JB, Velasquillo C, Ibarra C (2015) Regulation of α5 and αV Integrin Expression by GDF-5 and BMP-7 in Chondrocyte Differentiation and Osteoarthritis.
PLoS ONE 10(5): e0127166.
Mouse anti Human aggrecan antibody, clone 7E1 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).
Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).
Mouse anti Human aggrecan antibody, clone 7E1 recognizes a ~205 kDa trypsin derived fragment of aggrecan.
Note: Originally described as being of the isotype IgM, clone 7E1 was found to be unstable and was re-cloned by the originator and the new stable sub-clone was confirmed as being an IgG1.
Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).
Mouse anti Human aggrecan antibody, clone 7E1 recognizes a ~205 kDa trypsin derived fragment of aggrecan.
Note: Originally described as being of the isotype IgM, clone 7E1 was found to be unstable and was re-cloned by the originator and the new stable sub-clone was confirmed as being an IgG1.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Chicken Fish Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Purified human articular cartilage aggrecan.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
More Information
- UniProt
- P16112 Related reagents
- Entrez Gene
- ACAN Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0007155 cell adhesion
- GO:0005201 extracellular matrix structural constituent
- GO:0005529 sugar binding
- GO:0005540 hyaluronic acid binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0006508 proteolysis
- Regulatory
- For research purposes only
Applications of Aggrecan antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunocytochemistry | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Aggrecan antibody
-
Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
J Cell Mol Med. 13 (9B): 3151-73. -
Aigner, T. et al. (2002) Prognostic relevance of cell biologic and biochemical features in conventional chondrosarcomas.
Cancer. 94: 2273-81. -
Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Iranian J basic Med Res. 11: 10-17 -
Garciadiego-Cázares, D. et al. (2015) Regulation of α5 and αV Integrin Expression by GDF-5 and BMP-7 in Chondrocyte Differentiation and Osteoarthritis.
PLoS One. 10 (5): e0127166. -
Mukherjee, D.P. et al. (2009) Effect of 3D-microstructure of bioabsorbable PGA:TMC scaffolds on the growth of chondrogenic cells.
J Biomed Mater Res B Appl Biomater. 88 (1): 92-102. -
Wei, A. et al. (2016) Expression of growth differentiation factor 6 in the human developing fetal spine retreats from vertebral ossifying regions and is restricted to cartilaginous tissues.
J Orthop Res. 34 (2): 279-89.
