Aggrecan antibody | 7E1
Mouse anti Human aggrecan antibody, clone 7E1 (MCA1451G) used for the detection of aggrecan expressing cells in murine micromass cultures by immunocytochemistry.
Image caption:
Regulation of Extracellular matrix (ECM) composition by Bone morphogenetic protein (BMP) family growth factors during chondrogenesis. Mouse micromass cultures without treatment (A–E), Gdf-5 treatment (F–J), and Bmp-7 treatment (K–O); Alcian blue stain (A–K) showed cartilage nodules, Immunohistochemical stain for type 1 collagen (A, F, K), type II collagen (C, H, M), aggrecan (D, I, N), and fibronectin (E, J, O). Statistical analysis for histochemical and immunohistochemical data, (P) = percentage of positive area for Alcian blue, type I collagen, type II collagen, aggrecan, and fibronectin stains in micromasses with different treatments; mean values are shown with Standard deviations (SD) (n = three independent experiments), One-way Analysis of variance (ANOVA). ***P <0.0001; **p <0.001; *p <0.05). Gdf-5 mainly induces aggrecan expression, type II collagen, and fibronectin; aggrecan is an articular cartilage marker; in contrast, Bmp-7 induce type I collagen, an extracellular component of hypertrophic cartilage and bone.
From: Garciadiego-Cázares D, Aguirre-Sánchez HI, Abarca-Buis RF, Kouri JB, Velasquillo C, Ibarra C (2015)
Regulation of α5 and αV Integrin Expression by GDF-5 and BMP-7 in Chondrocyte Differentiation and Osteoarthritis.
PLoS ONE 10(5): e0127166.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Aggrecan
- Product Type
- Monoclonal Antibody
- Clone
- 7E1
- Isotype
- IgG1
- Specificity
- Aggrecan
Antibody Quality and Performance Guarantee
| Mouse anti Human aggrecan antibody, clone 7E1 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). A panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. There is a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009). Mouse anti Human aggrecan antibody, clone 7E1 recognizes a ~205 kDa trypsin derived fragment of aggrecan. Note: Originally described as being of the isotype IgM, clone 7E1 was found to be unstable and was re-cloned by the originator and the new stable sub-clone was confirmed as being an IgG1. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Chicken Fish Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified human articular cartilage aggrecan.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunocytochemistry | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Aggrecan antibody
-
Aigner, T. et al. (2002) Prognostic relevance of cell biologic and biochemical features in conventional chondrosarcomas.
Cancer. 94: 2273-81. -
Mukherjee, D.P. et al. (2009) Effect of 3D-microstructure of bioabsorbable PGA:TMC scaffolds on the growth of chondrogenic cells.
J Biomed Mater Res B Appl Biomater. 88 (1): 92-102. -
Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
J Cell Mol Med. 13 (9B): 3151-73. -
Garciadiego-Cázares, D. et al. (2015) Regulation of α5 and αV Integrin Expression by GDF-5 and BMP-7 in Chondrocyte Differentiation and Osteoarthritis.
PLoS One. 10 (5): e0127166. -
Wei, A. et al. (2016) Expression of growth differentiation factor 6 in the human developing fetal spine retreats from vertebral ossifying regions and is restricted to cartilaginous tissues.
J Orthop Res. 34 (2): 279-89.
- UniProt
- P16112
- Entrez Gene
- ACAN
- GO Terms
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0007155 cell adhesion
- GO:0005201 extracellular matrix structural constituent
- GO:0005529 sugar binding
- GO:0005540 hyaluronic acid binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0006508 proteolysis
MCA1451G
151699
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