A2M antibody | M17
Mouse anti Human A2M
- Product Type
- Monoclonal Antibody
- Clone
- M17
- Isotype
- IgG1
- Specificity
- A2M
| Mouse anti Human A2M antibody, clone M17, recognizes alpha-2-macroglobulin (A2M), also known as CPAMD5. A2M is a major proteinase inhibitor, with the ability to bind most proteinases and many growth factors, hormones and cytokines in human blood and tissue (Kurz et al. 2017). A2M is largely produced by the liver under conditions of stress, and is then secreted into the blood and extracellular spaces. It is also produced locally by macrophages, fibroblasts, and epithelial cells (Kim et al. 2017). A2M levels dramatically decrease as humans age, a process which has been hypothesized to facilitate tumor development. In naked mole rats, a species with pronounced resistance to cancer, A2M has been shown to inhibit tumor growth regardless of tumor origin (Kurz et al. 2017). The purified Mouse anti Human A2M antibody, clone M17 (MCA6216GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human A2M antibody, clone M5 (MCA6217B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human A2M (Ser24-Ala1474)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6217B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- Alpha-2-macroglobulin
- UniProt
- P01023
- Entrez Gene
- A2M
- GO Terms
- GO:0001869 negative regulation of complement activation, lectin pathway
- GO:0002576 platelet degranulation
- GO:0005829 cytosol
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005096 GTPase activator activity
- GO:0005615 extracellular space
- GO:0007264 small GTPase mediated signal transduction
- GO:0007597 blood coagulation, intrinsic pathway
- GO:0019899 enzyme binding
- View More GO Terms
- GO:0019959 interleukin-8 binding
- GO:0019966 interleukin-1 binding
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0043120 tumor necrosis factor binding
- GO:0051056 regulation of small GTPase mediated signal transduction
MCA6216GA
100005287
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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