MHC Class II Polymorphic antibody | CI.13.I
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Mouse anti Guinea Pig MHC Class II Polymorphic
- Product Type
- Monoclonal Antibody
- Clone
- CI.13.I
- Isotype
- IgG2b
- Specificity
- MHC Class II Polymorphic
Antibody Quality and Performance Guarantee
| Mouse anti Guinea Pig MHC class II polymorphic antibody, clone CI-13.1 recognizes the MHC Class II epitope present on strain 13, but not strain 2 guinea pig cells. The antigen is also found in outbred Hartley guinea pigs. |
- Target Species
- Guinea Pig
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- 0.22 micron filtered Tissue Culture Supernatant
- Preservative Stabilisers
0.1% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Guinea pig peritoneal T-cells.
- Fusion Partners
- Lymph node cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Source Reference
-
Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
Hybridoma. 4 (2): 115-24.
References for MHC Class II Polymorphic antibody
-
Liversidge, J. & Forrester, J.V. (1988) Experimental autoimmune uveitis (EAU): immunophenotypic analysis of inflammatory cells in chorio retinal lesions.
Curr Eye Res. 7 (12): 1231-41. -
Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55 (1): 124-31.
- RRID
- AB_324169
MCA753
161503
166191
180315
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with MHC CLASS II specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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