Mouse anti Dog MHC Class II Monomorphic antibody, clone CA2.1C12
recognizes a monomorphic epitope on canine MHC Class II which was classified at the First Canine Leucocyte Antigen Workshop (CLAW) [Cobbold et al. 1992
]. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MHC Class II Monomorphic antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for MHC Class II Monomorphic antibody
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and
functional differentiation. PubMed PMID:
Can J Vet Res. 71: 165-74.
Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
Vet J. 187 (3): 320-4.
Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Vet Pathol. 33: 204-12.
Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
J Leukoc Biol. 84: 1501-10.
Isotani, M. et al. (2006) Efficient generation of canine bone marrow-derived dendritic cells.
J Vet Med Sci. 68: 809-14.
Liu, C.C. et al. (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
Cancer Immunol Immunother. 57: 479-91.
McDonough, S.P. and Moore, P.F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
Vet Pathol. 37: 637-46.
Pumarola, M. et al. (2004) Canine inflammatory myopathy: analysis of cellular infiltrates.
Muscle Nerve. 29: 782-9.
Ricklin, Gutzwiller. M.E. et al. (2010) Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells.
Vet Res. 41: 40.
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60.
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