MHC Class II Monomorphic antibody | CA2.1C12
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Mouse anti Dog MHC Class II Monomorphic antibody, clone CA2.1C12 recognizes a monomorphic epitope on canine MHC Class II which was classified at the First Canine Leucocyte Antigen Workshop (CLAW) [Cobbold et al. 1992]. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region.
Product Details
- Target Species
- Dog
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II Monomorphic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for MHC Class II Monomorphic antibody
-
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. -
Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and
functional differentiation. PubMed PMID:
Can J Vet Res. 71: 165-74. -
Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
Vet J. 187 (3): 320-4. -
Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Vet Pathol. 33: 204-12. -
Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
J Leukoc Biol. 84: 1501-10. -
Isotani, M. et al. (2006) Efficient generation of canine bone marrow-derived dendritic cells.
J Vet Med Sci. 68: 809-14. -
Liu, C.C. et al. (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
Cancer Immunol Immunother. 57: 479-91. -
McDonough, S.P. and Moore, P.F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
Vet Pathol. 37: 637-46. -
Pumarola, M. et al. (2004) Canine inflammatory myopathy: analysis of cellular infiltrates.
Muscle Nerve. 29: 782-9. -
Ricklin, Gutzwiller. M.E. et al. (2010) Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells.
Vet Res. 41: 40. -
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60.
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