CD8 Beta antibody | CA15.4G2

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Mouse anti Dog CD8 Beta

Product Type
Monoclonal Antibody
Clone
CA15.4G2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1775S C* F IP 2 ml
Mouse anti Dog CD8 beta antibody, clone CA15.4G2 recognises the canine CD8β cell surface antigen, expressed by cytotoxic\suppressor T lymphocytes that interact with MHC Class I expressing targets.

Product Details

Target Species
Dog
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD8 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen 1 1/10
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD8 Beta antibody

  1. Luckschander, N. et al. (2009) Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes.
    Vet Res. 40: 58.
  2. Pumarola, M. et al. (2004) Canine inflammatory myopathy: analysis of cellular infiltrates.
    Muscle Nerve. 29: 782-9.
  3. Vernau, W. and Moore, P.F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
    Vet Immunol Immunopathol. 69: 145-64.
  4. Moreno, J. et al. (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
    Vet Immunol Immunopathol. 71: 181-95.
  5. Kisseberth, W.C. et al. (2007) A novel canine lymphoma cell line: a translational and comparative model for lymphoma research.
    Leuk Res. 31: 1709-20.
  6. Gauthier, M.J. et al. (2005) The immunophenotype of peripheral blood lymphocytes in clinically healthy dogs and dogs with lymphoma in remission.
    J Vet Intern Med. 19: 193-9.
  7. Sonea, I.M. et al. (2000) Flow cytometric analysis of colonic and small intestinal mucosal lymphocytes obtained by endoscopic biopsy in the healthy dog.
    Vet Immunol Immunopathol. 77: 103-19.
  8. Lau, K.W. et al. (1999) Large granular lymphocytic leukemia in a mixed breed dog.
    Can Vet J. 40: 725-8.
  9. Wilkerson, M.J. et al. (2005) Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules.
    Vet Immunol Immunopathol. 106: 179-96.
  10. Izci C et al. (2015) Clinical and light microscopic studies of the conjunctival tissues of dogs with bilateral keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine.
    Biotech Histochem. 90 (3): 223-30.

Further Reading

  1. Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T lymphocyte subsets and high density expression of CD4 by canine neutrophils.
    Tissue Antigens 40: 75-85.