CD8 Alpha antibody | CA9.JD3
Mouse anti Dog CD8 alpha antibody, clone CA9.JD3 recognizes the canine CD8 alpha chain which is expressed by thymocytes, peripheral T cells in the blood and lymphoid organs .
Mouse anti Dog CD8α clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.
Mouse anti Dog CD8α clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.
Product Details
- Target Species
- Dog
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Canine Thymocytes
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P33706
- Entrez Gene
- CD8A
- GO Terms
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunohistology - Frozen | Neat | 1/10 | |
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Spleen, lymph node
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for CD8 Alpha antibody
-
Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.
Tissue Antigens 40(2): 75-85. -
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. -
Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
Vet J. 187 (3): 320-4. -
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60. -
Huyghe, S. et al. (2015) The Microscopic Structure of the Omentum in Healthy Dogs: The Mystery Unravelled.
Anat Histol Embryol. Jul 23. [Epub ahead of print] -
Lin Shiow-Chen et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
Tai Vet J. 40 (04): 181-90. -
Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
Parasite Immunol. Jul 27 [Epub ahead of print]. -
Knebel, A. et al. (2021) Measurement of canine Th17 cells by flow cytometry.
Vet Immunol Immunopathol. 243: 110366.
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