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CD8 Alpha antibody | CA9.JD3

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Anti Dog CD8 Alpha Antibody, clone CA9.JD3 gallery image 1
Figure A. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Mouse IgG2a isotype control (MCA929) labeled with Goat anti Mouse Dylight 649 (STAR117D649). Figure B. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Mouse anti Dog CD8a (MCA1999S) labeled with Goat anti Mouse Dylight 649 (STAR117D649). All experiments performed on red cell lysed canine blood gated lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Dog CD8 Alpha

Product Type
Monoclonal Antibody
Clone
CA9.JD3
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1999S
Datasheet
C F IP
SDS
2 ml loader

Mouse anti Dog CD8 alpha antibody, clone CA9.JD3 recognizes the canine CD8 alpha chain which is expressed by thymocytes, peripheral T cells in the blood and lymphoid organs .

Mouse anti Dog CD8α clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.

Product Details

Target Species
Dog
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Canine Thymocytes
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P33706
Entrez Gene
CD8A
GO Terms
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of CD8 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen Neat 1/10
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Spleen, lymph node
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for CD8 Alpha antibody

  1. Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.
    Tissue Antigens 40(2): 75-85.
  2. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  3. Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
    Vet J. 187 (3): 320-4.
  4. Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
    Gene Ther. 14: 1249-60.
  5. Huyghe, S. et al. (2015) The Microscopic Structure of the Omentum in Healthy Dogs: The Mystery Unravelled.
    Anat Histol Embryol. Jul 23. [Epub ahead of print]
  6. Lin Shiow-Chen et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
    Tai Vet J. 40 (04): 181-90.
  7. Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
    Parasite Immunol. Jul 27 [Epub ahead of print].

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