Mouse anti Dog CD8 alpha antibody, clone CA9.JD3
recognizes the canine CD8 alpha chain which is expressed by thymocytes, peripheral T cells in the blood and lymphoid organs .
Mouse anti Dog CD8α clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994
). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Canine Thymocytes
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD8 Alpha antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Spleen, lymph node
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for CD8 Alpha antibody
Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.
Tissue Antigens 40(2): 75-85.
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
Vet J. 187 (3): 320-4.
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60.
Huyghe, S. et al. (2015) The Microscopic Structure of the Omentum in Healthy Dogs: The Mystery Unravelled.
Anat Histol Embryol. Jul 23. [Epub ahead of print]
Lin Shiow-Chen et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
Tai Vet J. 40 (04): 181-90.
Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
Parasite Immunol. Jul 27 [Epub ahead of print].
Knebel, A. et al. (2021) Measurement of canine Th17 cells by flow cytometry.
Vet Immunol Immunopathol. 243: 110366.
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