CD8 Alpha antibody | CA9.JD3
Mouse anti Dog CD8α clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.
Product Details
- Target Species
- Dog
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Canine Thymocytes
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P33706
- Entrez Gene
- CD8A
- GO Terms
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Immunohistology - Frozen | Neat | 1/10 | |
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Spleen, lymph node
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Product Specific References
References for CD8 Alpha antibody
-
Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.
Tissue Antigens 40(2): 75-85. -
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. -
Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
Vet J. 187 (3): 320-4. -
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60. -
Huyghe, S. et al. (2015) The Microscopic Structure of the Omentum in Healthy Dogs: The Mystery Unravelled.
Anat Histol Embryol. Jul 23. [Epub ahead of print] -
Lin Shiow-Chen et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
Tai Vet J. 40 (04): 181-90. -
Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
Parasite Immunol. Jul 27 [Epub ahead of print].
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