Mouse anti Dog CD45RA antibody, clone CA4.1D3
recognizes the canine homologue of CD45RA, clustered at the first International canine leukocyte Antigen Workshop (Cobbold et al. 1994
). Clone CA4.1D3 identifies a cell surface glycoprotein expressed by B lymphocytes, a subset of T-lymphocytes, monocytes and thymocytes (Moore et al. 1990
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RA antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD45RA antibody
Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Vet Pathol. 33 (2): 204-12.
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Bauer, T.R. Jr et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.
Blood. 108 (10): 3313-20.
Ting-De Ravin SS et al. (2006) Correction of canine X-linked severe combined immunodeficiency by in vivo retroviral gene therapy.
Blood. 107 (8): 3091-7.
Bauer, T.R. Jr et al. (2013) Long-term follow-up of foamy viral vector-mediated gene therapy for canine leukocyte adhesion deficiency.
Mol Ther. 21 (5): 964-72.
Rütgen BC et al. (2012) Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model.
PLoS One. 7 (6): e40078.
Alderberth, I et al. (2015) Prevention of Inflammatory Disorders in Domestic Non-Human Mammals
US Patent Publication number US20150079131 A1
Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
Vet Immunol Immunopathol. 164 (3-4): 127-36.
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