Mouse anti Dog CD45RA antibody, clone CA4.1D3
recognizes the canine homologue of CD45RA, clustered at the first International canine leukocyte Antigen Workshop (Cobbold et al. 1994
). Clone CA4.1D3 identifies a cell surface glycoprotein expressed by B lymphocytes, a subset of T-lymphocytes, monocytes and thymocytes (Moore et al. 1990
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RA antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD45RA antibody
Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Vet Pathol. 33 (2): 204-12.
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Bauer, T.R. Jr et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.
Blood. 108 (10): 3313-20.
Ting-De Ravin SS et al. (2006) Correction of canine X-linked severe combined immunodeficiency by in vivo retroviral gene therapy.
Blood. 107 (8): 3091-7.
Bauer, T.R. Jr et al. (2013) Long-term follow-up of foamy viral vector-mediated gene therapy for canine leukocyte adhesion deficiency.
Mol Ther. 21 (5): 964-72.
Rütgen BC et al. (2012) Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model.
PLoS One. 7 (6): e40078.
Alderberth, I et al. (2015) Prevention of Inflammatory Disorders in Domestic Non-Human Mammals
US Patent Publication number US20150079131 A1
Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
Vet Immunol Immunopathol. 164 (3-4): 127-36.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra