Mouse anti Dog CD45 antibody, clone CD12.10C12
recognises the canine homologue of CD45, clustered at the firstInternational Canine Leukocyte Workshop (Cobbold et al. 1994
). Clone CA12.10C12 recognises a glycoprotein of approximately 200 kDa expressed by cells of haemapoietic origin.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45 antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1 This product requires heat mediated antigen retrieval (citrate buffer, pH 6.0).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD45 antibody
Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Vet Pathol. 33 (2): 204-12.
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Ramos-Vara, J.A. & Miller, M.A. (2007) Immunohistochemical detection of protein gene product 9.5 (PGP 9.5) in canine epitheliotropic T-cell lymphoma (mycosis fungoides).
Vet Pathol. 44 (1): 74-9.
Caswell JL, et al. (2005) Graft-versus-host disease in severe combined immunodeficiency/beige mice administered canine leukocytes.
Can J Vet Res. 69(4): 246-52.
Lane, L.V. et al. (2012) Canine intravascular lymphoma with overt leukemia.
Vet Clin Pathol. Mar;41(1):84-91.
Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. 50 (6) Oct 08 [Epub ahead of print].
Tsumagari, S. et al. (2007) Characterization of CD34+ cells from canine umbilical cord blood, bone marrow leukocytes, and peripheral blood by flow cytometric analysis.
J Vet Med Sci. 69 (11): 1207-9.
Lombardi, P. et al. (2019) Clinical and Immunological Response in Dogs Naturally Infected by L. infantum Treated with a Nutritional Supplement.
Animals (Basel). 9 (8) Jul 30 [Epub ahead of print].
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