Mouse anti Dog CD45 antibody, clone CD12.10C12
recognises the canine homologue of CD45, clustered at the firstInternational Canine Leukocyte Workshop (Cobbold et al. 1994
). Clone CA12.10C12 recognises a glycoprotein of approximately 200 kDa expressed by cells of haemapoietic origin.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45 antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1 This product requires heat mediated antigen retrieval (citrate buffer, pH 6.0).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD45 antibody
Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Vet Pathol. 33 (2): 204-12.
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Ramos-Vara, J.A. & Miller, M.A. (2007) Immunohistochemical detection of protein gene product 9.5 (PGP 9.5) in canine epitheliotropic T-cell lymphoma (mycosis fungoides).
Vet Pathol. 44 (1): 74-9.
Caswell JL, et al. (2005) Graft-versus-host disease in severe combined immunodeficiency/beige mice administered canine leukocytes.
Can J Vet Res. 69(4): 246-52.
Lane, L.V. et al. (2012) Canine intravascular lymphoma with overt leukemia.
Vet Clin Pathol. Mar;41(1):84-91.
Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. 50 (6) Oct 08 [Epub ahead of print].
Tsumagari, S. et al. (2007) Characterization of CD34+ cells from canine umbilical cord blood, bone marrow leukocytes, and peripheral blood by flow cytometric analysis.
J Vet Med Sci. 69 (11): 1207-9.
Lombardi, P. et al. (2019) Clinical and Immunological Response in Dogs Naturally Infected by L. infantum Treated with a Nutritional Supplement.
Animals (Basel). 9 (8) Jul 30 [Epub ahead of print].