CD45 antibody | CA12.10C12

Mouse anti Dog CD45

Product Type
Monoclonal Antibody
Clone
CA12.10C12
Isotype
IgG1
Specificity
CD45

Product Code Applications Pack Size List Price Your Price Qty
MCA2035S
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 2 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Dog CD45 antibody, clone CD12.10C12recognises the canine homologue of CD45, clustered at the firstInternational Canine Leukocyte Workshop (Cobbold et al. 1994). Mouse anti Dog CD45 antibody, clone CD12.10C12 recognizes a glycoprotein of approximately 200 kDa expressed by cells of haemapoietic origin.

Target Species
Dog
Product Form
Tissue culture supernatant - liquid
Preservative Stabilisers
0.09% sodium azide (NaN3)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1 This product requires heat mediated antigen retrieval (citrate buffer, pH 6.0).
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for CD45 antibody

  1. Caniatti, M. et al. (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
    Vet Pathol. 33 (2): 204-12.
  2. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  3. Ramos-Vara, J.A. & Miller, M.A. (2007) Immunohistochemical detection of protein gene product 9.5 (PGP 9.5) in canine epitheliotropic T-cell lymphoma (mycosis fungoides).
    Vet Pathol. 44 (1): 74-9.
  4. Caswell JL, et al. (2005) Graft-versus-host disease in severe combined immunodeficiency/beige mice administered canine leukocytes.
    Can J Vet Res. 69(4): 246-52.
  5. Lane, L.V. et al. (2012) Canine intravascular lymphoma with overt leukemia.
    Vet Clin Pathol. Mar;41(1):84-91.
  6. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. 50(6):e12391.
  7. Tsumagari, S. et al. (2007) Characterization of CD34+ cells from canine umbilical cord blood, bone marrow leukocytes, and peripheral blood by flow cytometric analysis.
    J Vet Med Sci. 69 (11): 1207-9.
  8. Lombardi, P. et al. (2019) Clinical and Immunological Response in Dogs Naturally Infected by L. infantum Treated with a Nutritional Supplement.
    Animals (Basel). 9 (8) Jul 30 [Epub ahead of print].
  9. View The Latest Product References

Synonyms
LCA
RRID
AB_323369
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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