Find out more about A.I.den - our SearchBot

CD11d antibody | CA11.8H2

100% Secure
Anti Dog CD11d Antibody, clone CA11.8H2 thumbnail image 1 Anti Dog CD11d Antibody, clone CA11.8H2 thumbnail image 2 Anti Dog CD11d Antibody, clone CA11.8H2 thumbnail image 3
Anti Dog CD11d Antibody, clone CA11.8H2 gallery image 1
Staining of canine peripheral blood lymphocytes with Mouse anti Dog CD11d:Alexa Fluor® 647 (MCA1779A647)
Anti Dog CD11d Antibody, clone CA11.8H2 gallery image 2
Staining of canine peripheral blood lymphocytes with Mouse anti Dog CD11d (MCA1779GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)
Anti Dog CD11d Antibody, clone CA11.8H2 gallery image 3
Figure A. RPE conjugated Rat anti Dog CD8 (MCA1039PE) and isotype control detected with FITC conjugated Goat anti Mouse IgG (STAR117F). Figure B. RPE conjugated Rat anti Dog CD8 (MCA1039PE) and Mouse anti Dog CD11d (MCA1779GA) detected with FITC conjugated Goat anti Mouse IgG (STAR117F). All experiments performed on red cell lysed dog blood gated on lymphocytes in the presence of 10% dog serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Dog CD11d

Product Type
Monoclonal Antibody
Clone
CA11.8H2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1779GA
Datasheet
C* F IP
SDS
0.1 mg loader

Product Details

Target Species
Dog
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD11d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD11d antibody

  1. Danilenko, D.M. et al. (1995) A novel canine leukointegrin, alpha d beta 2, is expressed by specific macrophage subpopulations in tissue and a minor CD8+ lymphocyte subpopulation in peripheral blood.
    J Immunol. 155:35-44.
  2. Moore, P.F. et al. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
    Vet Pathol. 43:632-45.
  3. Vernau, W. and Moore, P.F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
    Vet Immunol Immunopathol. 69:145-64.
  4. Fry, M.M. et al. (2003) Hepatosplenic lymphoma in a dog.
    Vet Pathol. 40:556-62.
  5. Stokol, T. et al. (2015) Alkaline phosphatase is a useful cytochemical marker for the diagnosis of acute myelomonocytic and monocytic leukemia in the dog.
    Vet Clin Pathol. 44 (1): 79-93.
  6. Ortiz, A.L. et al. (2015) Gamma delta T-cell large granular lymphocyte lymphoma in a dog.
    Vet Clin Pathol. 44 (3): 442-7.

Further Reading

  1. Vernau. W. (2004) Flow Cytometric Assessment of Hematopoietic Neoplasia in the Dog.
    In: 55th Annual Meeting of the American College of Veterinary Pathologists (ACVP) & 39th Annual Meeting of the American Society of Clinical Pathology (ASVCP),

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
Request a different product with the CD11d specificity