CD11d antibody | CA11.8H2
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Mouse anti Dog CD11d
- Product Type
- Monoclonal Antibody
|Mouse anti Dog CD11d antibody, clone CA11.8H2 recognizes the canine CD11d cell surface antigen, also known as integrin alpha d subunit. The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp.
Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages (Moore et al. 2006) with macrophages demonstrating marked erythrocytic phagocytosis. Previously, canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ γ/δ T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL (Fry et al. 2003).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Affinity purified CD11 antigen from canine spleen.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
Danilenko, D.M. et al. (1995) A novel canine leukointegrin, alpha d beta 2, is expressed by specific macrophage subpopulations in tissue and a minor CD8+ lymphocyte subpopulation in peripheral blood.
J Immunol. 155:35-44.
References for CD11d antibody
Moore, P.F. et al. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
Vet Pathol. 43:632-45.
Vernau, W. and Moore, P.F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
Vet Immunol Immunopathol. 69:145-64.
Fry, M.M. et al. (2003) Hepatosplenic lymphoma in a dog.
Vet Pathol. 40:556-62.
Ortiz, A.L. et al. (2015) Gamma delta T-cell large granular lymphocyte lymphoma in a dog.
Vet Clin Pathol. 44 (3): 442-7.
- Integrin Alpha D Chain
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