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CD11c antibody | CA11.6A1

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Anti Dog CD11c Antibody, clone CA11.6A1 thumbnail image 1 Anti Dog CD11c Antibody, clone CA11.6A1 thumbnail image 2
Anti Dog CD11c Antibody, clone CA11.6A1 gallery image 1
Figure A. PE conjugated Mouse anti Canine CD45 (MCA1042PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11c (MCA1778S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on monocytes.
Anti Dog CD11c Antibody, clone CA11.6A1 gallery image 2
Published customer image:
Mouse anti Canine CD11c antibody, clone CA11.6A1 (MCA1778S) used to evaluate CD11c expression on canine cell populations by flow cytometry.
Image caption:
Phenotype of monocytes, monocyte-derived MΦ (MF) and MoDC. The filled histogram represents the conjugated controls, the overlaid lines the expression of the markers of unstimulated (dashed line) or LPS (10 μg/mL) stimulated cells (solid line) (only for MΦ and MoDC). The numbers give the percentages of positive cells calculated with the Overtone algorithm of the Flowjo software. Data are representative of 5 independent experiments. A statistical significance for the LPS stimulation is indicated by a * (p < 0.05, n = 5, assessed for CD11c).

From: Ricklin Gutzwiller ME, Moulin HR, Zurbriggen A, Roosje P, Summerfield A.
Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells.
Vet Res. 2010 Jul-Aug;41(4):40.
doi: 10.1051/vetres/2010012.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Dog CD11c

Product Type
Monoclonal Antibody
Clone
CA11.6A1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1778S
Datasheet
C* F IP
SDS
2 ml loader

Mouse anti Dog CD11c antibody, clone CA11.6A1 recognizes the canine CD11c cell surface antigen, a member of the alpha integrin family. Canine CD11c is expressed by monocytes, granulocytes and by dendritic cells.

Mouse anti Dog CD11c, clone CA11.6A1 immunoprecipitates proteins of approximately 95 kDa, corresponding to the common β chain of the CD11/CD18 heterodimer and ~150 kDa, the CD11c; chain from canine leukocyte preparations (Danilenko et al. 1992)

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hooded Seal
Raccoon
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD11c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD11c antibody

  1. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMS): characterization of the CD11/CD18 family.
    Tissue Antigens 40: 13-21.
  2. Kang, J.W. et al. (2008) Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells.
    Stem Cells Dev. 17: 681-93.
  3. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
    Vet Pathol. 39: 74-83.
  4. Bird, R.C. et al. (2008) An allogeneic hybrid-cell fusion vaccine against canine mammary cancer.
    Vet Immunol Immunopathol. 123: 289-304.
  5. Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.
    J Comp Pathol. 126: 238-41.
  6. Isotani, M. et al. (2006) Efficient generation of canine bone marrow-derived dendritic cells.
    J Vet Med Sci. 68: 809-14.
  7. Liu, C.C. et al. (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
    Cancer Immunol Immunother. 57: 479-91.
  8. McDonough, S.P. and Moore, P.F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
    Vet Pathol. 37: 637-46.
  9. Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation.
    Can J Vet Res. 71: 165-74.
  10. Mathes, M. et al. (2006) Evaluation of liposomal clodronate in experimental spontaneous autoimmune hemolytic anemia in dogs.
    Exp Hematol. 34: 1393-402.
  11. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
    Am J Trop Med Hyg. 70: 618-24.
  12. Ricklin Gutzwiller, M.E. et al. (2010) Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells.
    Vet Res. 41: 40.
  13. Ibisch, C. et al. (2005) Functional canine dendritic cells can be generated in vitro from peripheral blood mononuclear cells and contain a cytoplasmic ultrastructural marker.
    J Immunol Methods. 298: 175-82.
  14. Wang, Y.S. et al. (2008) Cytokine profiles of canine monocyte-derived dendritic cells as a function of lipopolysaccharide- or tumor necrosis factor-alpha-induced maturation.
    Vet Immunol Immunopathol. 118: 186-98.
  15. Schwartz, M. et al. (2008) Selective CD11a upregulation on neutrophils in the acute phase of steroid-responsive meningitis-arteritis in dogs.
    Vet Immunol Immunopathol. 126: 248-55.
  16. Pai, C.C. et al. (2011) Immunopathogenic behaviors of canine transmissible venereal tumor in dogs following an immunotherapy using dendritic/tumor cell hybrid.
    Vet Immunol Immunopathol. 139 (2-4): 187-99.
  17. Figueiredo, M.M. et al. (2013) Expression of Toll-like Receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
    BMC Immunol. 14: 22.
  18. Larsen, A.K. et al. (2013) Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro.
    PLoS One. 8: e70186.
  19. Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
    Vet Immunol Immunopathol. 168 (3-4): 140-6.
  20. Paoloni, M. et al. (2015) Defining the Pharmacodynamic Profile and Therapeutic Index of NHS-IL12 Immunocytokine in Dogs with Malignant Melanoma.
    PLoS One. 10 (6): e0129954.
  21. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
    Immunobiology. 221 (1): 12-22.
  22. Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
    Parasite Immunol. Jul 27 [Epub ahead of print].
  23. Stokol, T. et al. (2015) Alkaline phosphatase is a useful cytochemical marker for the diagnosis of acute myelomonocytic and monocytic leukemia in the dog.
    Vet Clin Pathol. 44 (1): 79-93.
  24. Heinrich, F. et al. (2015) Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
    Vet Immunol Immunopathol. 163 (1-2): 86-92.
  25. Qeska, V. et al. (2014) Canine distemper virus infection leads to an inhibitory phenotype of monocyte-derived dendritic cells in vitro with reduced expression of co-stimulatory molecules and increased interleukin-10 transcription.
    PLoS One. 9 (4): e96121.
  26. Bird, R.C. et al. (2019) Autologous hybrid cell fusion vaccine in a spontaneous intermediate model of breast carcinoma.
    J Vet Sci. 20 (5): e48.

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