CD11b antibody | CA16.3E10

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Mouse anti Dog CD11b

Product Type
Monoclonal Antibody
Clone
CA16.3E10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1777S C* F IP 2 ml
Mouse anti Dog CD11b antibody, clone CA16.3E10 is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family. CD11b forms one of the possible alpha chains of the canine leukocyte adhesion complexes (LeuCAMs), these contain a common 95 kDa β chain (CD18) non-covalently bound to either a 150 kDa (CD11c), 165 kDa (CD11b) or 180 kDa (CD11a) α chain (Moore et al. 1990. The CD11/CD18 complex is also known as the CR3 receptor.

Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti Dog CD11b (CA16.3E10) has been used to evaluate the effect of anesthetic administration of CD11b expression on canine neutrophils (Maeda et al. 2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium.

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Cat
Mustelid
Pig
Bovine
Mink
Beluga whale
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Affinity purified beta-2 integrins from splenic lysate

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD11b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested recommended dilutions are given as guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD11b antibody

  1. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMs): characterization of the CD11/CD18 family.
    Tissue Antigens 40: 13-21.
  2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  3. Sampaio, W.M. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
    BMC Vet Res. 3:11.
  4. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
  5. Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
    Gene Ther. 14: 1249-60.
  6. Kamstock, D. et al. (2006) Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma.
    Cancer Gene Ther. 13: 306-17.
  7. Sherger, M. et al. (2012) Identification of myeloid derived suppressor cells in the peripheral blood of tumor bearing dogs.
    BMC Vet Res. 8: 209.
  8. Gregorevic, P. et al. (2009) Evaluation of vascular delivery methodologies to enhance rAAV6-mediated gene transfer to canine striated musculature.
    Mol Ther. 17: 1427-33.
  9. Figueiredo, M.M. et al. (2013) Expression of Toll-like Receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
    BMC Immunol. 14: 22.
  10. Thompson, L.A. and Romano, T.A. (2015) Beluga (Delphinapterus leucas) granulocytes and monocytes display variable responses to in vitro pressure exposures.
    Front. Physiol. 6:128.
  11. Kuraoka, M. et al. (2016) Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
    Am J Pathol. 186 (5): 1302-12.
  12. Gow, A.G. et al. (2016) Low-Density Lipoprotein Uptake Demonstrates a Hepatocyte Phenotype in the Dog, but Is Nonspecific.
    Stem Cells Dev. 25 (1): 90-100.
  13. Paltrinieri, S. et al. (2012) Flow cytometric detection of alpha-1-acid glycoprotein on feline circulating leucocytes.
    Aust Vet J. 90 (8): 291-6.
  14. Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.
    Vet Immunol Immunopathol. 155 (3): 211-7.
  15. Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
    Vet Microbiol. 164 (1-2): 46-59.
  16. Guth, A.M. et al. (2013) Liposomal clodronate treatment for tumour macrophage depletion in dogs with soft-tissue sarcoma.
    Vet Comp Oncol. 11 (4): 296-305.
  17. Wijewardana, V. et al. (2013) Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy.
    Vet Immunol Immunopathol. 156 (1-2): 121-7.
  18. Mastrorilli, C. et al. (2012) Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping.
    Vet Clin Pathol. 41 (3): 412-8.
  19. Yu, D.H. et al. (2012) Pathophysiologic and immunologic changes in a canine endotoxemia over a period of 24 hours.
    J Vet Med Sci. 74 (5): 537-44.
  20. Olyslaegers, D.A. et al. (2013) Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis.
    Vet Microbiol. 166 (3-4): 438-49.
  21. Wasserman, J. et al. (2012) Suppression of canine myeloid cells by soluble factors from cultured canine tumor cells.
    Vet Immunol Immunopathol. 145 (1-2): 420-30.
  22. Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
    Dev Comp Immunol. 27 (10): 889-97.
  23. Kuraoka, M. et al. (2016) Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
    Am J Pathol. 186 (5): 1302-12.