CD11b antibody | CA16.3E10












Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti Dog CD11b antibody, clone CA16.3E10 has been used to evaluate the effect of anesthetic administration of CD11b expression on canine neutrophils (Maeda et al. 2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium.
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Cat Mustelid Pig Bovine Mink Beluga whale - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Affinity purified beta-2 integrins from splenic lysate
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD11b antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Immunoprecipitation |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Product Specific References
References for CD11b antibody
-
Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMs): characterization of the CD11/CD18 family.
Tissue Antigens 40: 13-21. -
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13. -
Sampaio, W.M. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
BMC Vet Res. 3:11. -
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. -
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60. -
Kamstock, D. et al. (2006) Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma.
Cancer Gene Ther. 13: 306-17. -
Sherger, M. et al. (2012) Identification of myeloid derived suppressor cells in the peripheral blood of tumor bearing dogs.
BMC Vet Res. 8: 209. -
Gregorevic, P. et al. (2009) Evaluation of vascular delivery methodologies to enhance rAAV6-mediated gene transfer to canine striated musculature.
Mol Ther. 17: 1427-33. -
Figueiredo, M.M. et al. (2013) Expression of Toll-like Receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
BMC Immunol. 14: 22. -
Thompson, L.A. & Romano, T.A. (2015) Beluga (Delphinapterus leucas) granulocytes and monocytes display variable responses to in vitro. pressure exposures.
Front Physiol. 6: 128. -
Kuraoka, M. et al. (2016) Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
Am J Pathol. 186 (5): 1302-12. -
Gow, A.G. et al. (2016) Low-Density Lipoprotein Uptake Demonstrates a Hepatocyte Phenotype in the Dog, but Is Nonspecific.
Stem Cells Dev. 25 (1): 90-100. -
Paltrinieri, S. et al. (2012) Flow cytometric detection of alpha-1-acid glycoprotein on feline circulating leucocytes.
Aust Vet J. 90 (8): 291-6. -
Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.
Vet Immunol Immunopathol. 155 (3): 211-7. -
Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
Vet Microbiol. 164 (1-2): 46-59. -
Guth, A.M. et al. (2013) Liposomal clodronate treatment for tumour macrophage depletion in dogs with soft-tissue sarcoma.
Vet Comp Oncol. 11 (4): 296-305. -
Wijewardana, V. et al. (2013) Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy.
Vet Immunol Immunopathol. 156 (1-2): 121-7. -
Mastrorilli, C. et al. (2012) Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping.
Vet Clin Pathol. 41 (3): 412-8. -
Yu, D.H. et al. (2012) Pathophysiologic and immunologic changes in a canine endotoxemia over a period of 24 hours.
J Vet Med Sci. 74 (5): 537-44. -
Olyslaegers, D.A. et al. (2013) Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis.
Vet Microbiol. 166 (3-4): 438-49. -
Wasserman, J. et al. (2012) Suppression of canine myeloid cells by soluble factors from cultured canine tumor cells.
Vet Immunol Immunopathol. 145 (1-2): 420-30. -
Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Dev Comp Immunol. 27 (10): 889-97. -
Kuraoka, M. et al. (2016) Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
Am J Pathol. 186 (5): 1302-12. -
Beirão, B.C.B. et al. (2020) A blocking antibody against canine CSF-1R maturated by limited CDR mutagenesis
Antibody Ther: 3.3: 193–204.
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