CD11b antibody | CA16.3E10
CD11b+CD14−MHCII− cells suppress T cell proliferation. Facs sorted CD11b+CD14−MHCII− cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Image caption:
Mouse anti-CD11b and Gr-1 antibodies cross-react with canine samples. Fresh PBMCs from healthy dog and cancer patients were isolated by Ficoll, stained with anti-mouse CD11b and anti-mouse Gr-1 antibodies.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
Image caption:
CD11b+CD14+MHCII− cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII− cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean ± SEM are shown.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
Image caption:
Flow cytometer analysis of CD11b integrin expression and the binding of Leishmania promastigotes to canine monocytes. (A) Gate R1 was built separating monocytes from animals naturally infected (ANI) with L. chagasi by cells size (x axis) and granularity (y axis). (B) Gate with mononuclear cells (control) (C) CD11b positive cells; (D) CD11b expression during the L. chagasi binding to mononuclear cells with the presence of C5D serum. (E) CD11b expression during the L. chagasi binding to mononuclear cells with the absence of C5D serum.
From: Sampaio et al. BMC Veterinary Research 2007 3:11
Image caption:
Published customer image:
Staining characteristics and evaluation of two commercially available canine granulocytic antibodies of canine peripheral blood. (a) Cells were analyzed according to forward and side scatter. Cells were either gated to include all live and dead (no gate), all live cells including lymphocytes (R1, lymphocytes are circled) and all myeloid cells (P1). (b) Cells were labeled using canine anti-CD11b and either (a) CADO48A or (b) DH59B with the same concentration of secondary antibody FITC. As can be seen in (a), CADO48A allows visualization of an additional cell population as compared to DH59B (b). These results were repeatable with multiple blood samples from different dogs. The cells in (b) were gated on P1 as indicated in (a).
From: Sherger et al. BMC Veterinary Research 2012 8:209
Image caption:
Optimization of secondary antibody staining concentration for detection of CADO48A. Cells were stained with the primary antibody CADO48A alone followed by (a) 1:10 (b) 1:50 and (c) 1:100 dilutions of a secondary FITC antibody. Optimal detection was seen between 1:50 and 1:100 dilution with CADO48A alone. Secondary FITC concentrations of 1:50 (d) and 1:100 (e) to detect CADO48A on pre-labeled CD11b cells were then evaluated and demonstrated that a 1:50 concentration of FITC was optimal for optimal detection of distinct CADO48A+ cells. Based on these findings, a 1:50 FITC concentration was used in all clinical samples. All cells in these diagrams were gated on P1.
From: Sherger et al. BMC Veterinary Research 2012 8:209
Image caption:
Effects of EDTA versus media storage of patient blood samples on flow cytometric results. Peripheral blood cells were (a) stained immediate following collection with CD11b and CADO48A or maintained in EDTA collection tubes for (b) 24 or (c) 48 hours or in media for (d) 24 or (e) 48 hours prior to staining with CD11b and CADO48A. Both EDTA and media samples were kept refrigerated. These findings show a decrease over time of population distinction in EDTA with minimal changes when cells are kept in media up to 48 hours. All cells were gated on P1.
From: Sherger et al. BMC Veterinary Research 2012 8:209
Image caption:
Effects of fixation on CD11b and CADO48A expression levels. Cells were stained for CD11b and CADO48A and evaluated pre-fixation (a) or subsequently fixed with 4% paraformaldehyde at (b) 4 hours, (c) 24 hours), (d) 48 hours or (e) 72 hours post-fixation before analysis on the flow cytometer. Fixation of cells appears to have limited effects on expression levels of CD11b and CADO48A expression up to 24 hours and then only mild increases of 4-6% of CD11b+CADO48A+ cells are seen at 24, 48 and 72 hours post-fixation. All cells seen in this diagram were gated on P1.
From: Sherger et al. BMC Veterinary Research 2012 8:209
Image caption:
Gated myeloid subpopulations evaluated for patient population. All peripheral blood myeloid cells were evaluated using the P1 gate (non-lymphocytes) and subsequently gated based on relatively expression levels of CD11b and CADO48A where CADO48Ahi/CD11bhi (gate P2), CADO48Alow/CD11bhi (gate P3) and CADO48Alow/CD11blow (gate P4) likely represent granulocytes/neutrophils, monocytes and MDSCs, respectively.
From: Sherger et al. BMC Veterinary Research 2012 8:209
Image caption:
Increased percentage of CD11blow/CADO48Alow in tumor-bearing canine patients. The expression levels of CD11blow and CADO48Alow was evaluated from the peripheral blood of representative canine patients and show an increase of CD11blow/CADO48low in tumor-bearing canine patients.
From: Sherger et al. BMC Veterinary Research 2012 8:209
Image caption:
Representative gating strategies demonstrating fluorescence profiles of cells in jejunum and colon lamina propria cells in dogs naturally infected with Leishmania; (A) macrophages were identified on the basis of their specific forward (FSC) and side (SSC) light-scattering properties (B) isotype control cutoff; (G) frequency of CD11b+; (H) frequency of CD11b+/CD14+; (I) frequency of TLR9+/CD11b+; (J) frequency of TLR9+/CD14+.
From: Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum. BMC Immunol. 2013 May 14;14:22.
Mouse anti Dog CD11b antibody, clone CA16.3E10 is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family. CD11b forms one of the possible alpha chains of the canine leukocyte adhesion complexes (LeuCAMs), these contain a common 95 kDa β chain (CD18) non-covalently bound to either a 150 kDa (CD11c), 165 kDa (CD11b) or 180 kDa (CD11a) α chain (Moore et al. 1990. The CD11/CD18 complex is also known as the CR3 receptor.
Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti Dog CD11b (CA16.3E10) has been used to evaluate the effect of anesthetic administration of CD11b expression on canine neutrophils (Maeda et al. 2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium.
Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti Dog CD11b (CA16.3E10) has been used to evaluate the effect of anesthetic administration of CD11b expression on canine neutrophils (Maeda et al. 2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium.
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Cat Mustelid Pig Bovine Mink Beluga whale - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Affinity purified beta-2 integrins from splenic lysate
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD11b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested recommended dilutions are given as guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD11b antibody
-
Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMs): characterization of the CD11/CD18 family.
Tissue Antigens 40: 13-21. -
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13. -
Sampaio, W.M. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
BMC Vet Res. 3:11. -
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. -
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60. -
Kamstock, D. et al. (2006) Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma.
Cancer Gene Ther. 13: 306-17. -
Sherger, M. et al. (2012) Identification of myeloid derived suppressor cells in the peripheral blood of tumor bearing dogs.
BMC Vet Res. 8: 209. -
Gregorevic, P. et al. (2009) Evaluation of vascular delivery methodologies to enhance rAAV6-mediated gene transfer to canine striated musculature.
Mol Ther. 17: 1427-33. -
Figueiredo, M.M. et al. (2013) Expression of Toll-like Receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
BMC Immunol. 14: 22. -
Thompson, L.A. and Romano, T.A. (2015) Beluga (Delphinapterus leucas) granulocytes and monocytes display variable responses to in vitro pressure exposures.
Front. Physiol. 6:128. -
Kuraoka, M. et al. (2016) Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
Am J Pathol. 186 (5): 1302-12. -
Gow, A.G. et al. (2016) Low-Density Lipoprotein Uptake Demonstrates a Hepatocyte Phenotype in the Dog, but Is Nonspecific.
Stem Cells Dev. 25 (1): 90-100. -
Paltrinieri, S. et al. (2012) Flow cytometric detection of alpha-1-acid glycoprotein on feline circulating leucocytes.
Aust Vet J. 90 (8): 291-6. -
Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.
Vet Immunol Immunopathol. 155 (3): 211-7. -
Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
Vet Microbiol. 164 (1-2): 46-59. -
Guth, A.M. et al. (2013) Liposomal clodronate treatment for tumour macrophage depletion in dogs with soft-tissue sarcoma.
Vet Comp Oncol. 11 (4): 296-305. -
Wijewardana, V. et al. (2013) Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy.
Vet Immunol Immunopathol. 156 (1-2): 121-7. -
Mastrorilli, C. et al. (2012) Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping.
Vet Clin Pathol. 41 (3): 412-8. -
Yu, D.H. et al. (2012) Pathophysiologic and immunologic changes in a canine endotoxemia over a period of 24 hours.
J Vet Med Sci. 74 (5): 537-44. -
Olyslaegers, D.A. et al. (2013) Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis.
Vet Microbiol. 166 (3-4): 438-49. -
Wasserman, J. et al. (2012) Suppression of canine myeloid cells by soluble factors from cultured canine tumor cells.
Vet Immunol Immunopathol. 145 (1-2): 420-30. -
Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Dev Comp Immunol. 27 (10): 889-97. -
Kuraoka, M. et al. (2016) Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
Am J Pathol. 186 (5): 1302-12.
