CD21 antibody | CC51

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Mouse anti Bovine CD21:FITC

Mouse anti Bovine CD21

Mouse anti Bovine CD21:RPE

Product Type
Monoclonal Antibody
Clone
CC51
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5953F F 0.1 mg
MCA5953GA F 0.1 mg
MCA5953PE F 100 Tests
Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012).

Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
African Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Bovine (Friesian cattle) mesenteric lymph node cells
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
12 months from date of reconstitution

More Information

UniProt
Q8HY44 Related reagents
Regulatory
For research purposes only

Applications of CD21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) STAR117D405GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F 100 Tests F
Mouse IgG2b Negative Control MCA691 100 Tests F
Mouse IgG2b Negative Control:RPE MCA691PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Pig wCD8 Alpha:RPE MCA1223PE 100 Tests F
Rat anti Human CD3:Pacific Blue® MCA1477PB 100 Tests/1ml F*
Mouse anti Human CD14:Pacific Blue® MCA1568PB 100 Tests/1ml F
Mouse anti Human CD14:RPE MCA1568PE 100 Tests F
Mouse anti Bovine CD4:RPE MCA1653PE 100 Tests F
Mouse anti Pig CD25 MCA1736GA 0.1 mg C F
Mouse anti Pig CD4 Alpha:RPE MCA1749PE 100 Tests F
Mouse anti Pig SLA Class II DR MCA2314GA 0.1 mg C F IP
Mouse anti Bovine CD25:RPE MCA2430PE 100 Tests F
Mouse anti Bovine MHC Class II DR:RPE MCA5656PE 100 Tests F
Mouse anti Pig CD3 MCA5951GA 0.1 mg C F IP WB
Mouse anti Pig CD3:RPE MCA5951PE 100 Tests F
Mouse anti Pig CD14:FITC MCA1218F 0.1 mg F
Mouse anti Pig wCD8 Alpha:RPE MCA1223PE 100 Tests F
Rat anti Human CD3:Pacific Blue® MCA1477PB 100 Tests/1ml F*
Mouse anti Pig CD4 Alpha MCA1749GA 0.1 mg C* F
Mouse anti Pig SLA Class II DR:FITC MCA2314F 0.1 mg F
Mouse anti Bovine CD25:RPE MCA2430PE 100 Tests F
Mouse anti Bovine MHC Class II DR:RPE MCA5656PE 100 Tests F
Mouse anti Pig CD3:RPE MCA5951PE 100 Tests F

Product Specific References

References for CD21 antibody

  1. Sinkora, M. et al. (2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.
    Vet Res. 45: 91.
  2. Sinkora, M et al. (2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.
    Dev Comp Immunol. 39: 409-18.
  3. Tenaya I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
    Vet Immunol Immunopathol. 149: 167-76.
  4. Denham S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.
    Vet Immunol Immunopathol. 43: 259-67.
  5. Boersma W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
    Vet Immunol Immunopathol. 80: 63-78.
  6. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes.
    Immunology. 69 (4): 525-30.