CD21 antibody | CC51
Mouse anti Bovine CD21
- Product Type
- Monoclonal Antibody
- Clone
- CC51
- Isotype
- IgG2b
- Specificity
- CD21
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012). Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig African Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine (Friesian cattle) mesenteric lymph node cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | STAR117D405GA | F IF | 0.1 mg |
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| Description | Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg |
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| Description | Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
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| Description | Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| Description | Mouse IgG2b Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD14:FITC | MCA1218F | F | 0.1 mg |
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| Description | Mouse anti Pig CD14:FITC | ||||||
| Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Pig wCD8 Alpha:RPE | ||||||
| Rat anti Human CD3:Pacific Blue® | MCA1477PB | F * | 100 Tests/1ml |
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| Description | Rat anti Human CD3:Pacific Blue® | ||||||
| Mouse anti Pig CD4 Alpha | MCA1749GA | C * F | 0.1 mg |
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| Description | Mouse anti Pig CD4 Alpha | ||||||
| Mouse anti Pig SLA Class II DR:FITC | MCA2314F | F | 0.1 mg |
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| Description | Mouse anti Pig SLA Class II DR:FITC | ||||||
| Mouse anti Bovine CD25:RPE | MCA2430PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Bovine CD25:RPE | ||||||
| Mouse anti Bovine MHC Class II DR:RPE | MCA5656PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Bovine MHC Class II DR:RPE | ||||||
| Mouse anti Pig CD3:RPE | MCA5951PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD3:RPE | ||||||
Source Reference
-
Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes.
Immunology. 69 (4): 525-30.
References for CD21 antibody
-
Denham S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.
Vet Immunol Immunopathol. 43: 259-67. -
Boersma W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80: 63-78. -
Tenaya I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
Vet Immunol Immunopathol. 149: 167-76. -
Sinkora, M et al. (2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.
Dev Comp Immunol. 39: 409-18. -
Sinkora, M. et al. (2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.
Vet Res. 45: 91. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Li, J. et al. (2023) Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells.
Genes Genomics. Nov 18 [Epub ahead of print]. -
Seemann, L. et al. (2024) Dietary L-carnitine supplementation modifies blood parameters of mid-lactating dairy cows during standardized lipopolysaccharide-induced inflammation.
Front Immunol. 15: 1390137.
View The Latest Product References
-
Yuan, C. et al. (2024) Effects of porcine epidemic diarrhea virus infection on CD21(+) B cells activation.
Vet Microbiol. 293: 110087.
- Synonyms
- CR2
- UniProt
- Q8HY44
MCA5953GA
0614
154523
163139
168042
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