CD268 / BAFF-R antibody | 2C4

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Mouse anti Chicken CD268 / BAFF-R

Product Type
Monoclonal Antibody
Clone
2C4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6011GA C F P 0.1 mg
Mouse anti Chicken CD268 / BAFF-R, clone 2C4, recognizes the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells.

Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 has been shown to cross react with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms.

Mouse anti Chicken CD268 / BAFF-R, clone 2C4 is unique in that it can be used in formalin fixed paraffin embbeded tissues and is therefore of particular use on sections from infection studies.

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
Quail
Duck
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Chicken BAFF-R transfected HEK293 cells
Approx. Protein Concentrations
IgG concentration1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD268 / BAFF-R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Chicken Bu-1A:FITC MCA2170F 0.1 mg F
Mouse anti Chicken Bu-1a/b MCA5764 0.25 mg C F IP
Mouse anti Guinea Fowl Bu-1a/b MCA5958GA 0.1 mg C IP WB

Product Specific References

Further Reading

  1. Reddy, S.K. et al. (2008) The BAFF-Interacting receptors of chickens.
    Dev Comp Immunol. 32 (9): 1076-87.
  2. Kothlow, S. et al. (2010) Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo.
    Mol Immunol. 47 (7-8): 1619-28.
  3. Schneider, K. et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
    Int Immunol. 16 (1): 139-48.
  4. Koskela, K. et al. (2004) Chicken B-cell-activating factor: regulator of B-cell survival in the bursa of fabricius.
    Scand J Immunol. 59 (5): 449-57.