CD268 / BAFF-R antibody | 2C4
Paraffin sections of bursal tissue were stained with the mouse anti chicken CD268 / BAFF-R antibody, clone 2C4 (2.5 μg/ml) followed by horse anti mouse Ig secondary antibody conjugated to biotin. Visualization was undertaken using a Vector ABC Kit and peroxidase activity was developed using 3.3´ diamino-benzidine-tetrahydro-chloride (DAB).
Image courtesy of Sonja Härtle, Institute for Animal Physiology, University of Munich
Peripheral blood lymphocytes were separated by density centrifugation and first stained with the mouse anti chBAFF-R (clone 2C4) and then with goat anti mouse IgG1 secondary antibody conjugated to FITC, finally blocked with 10% normal mouse serum and followed by staining with the mouse anti chicken Bu-1a/b (clone AV20) conjugated to APC.
Image courtesy of Sonja Härtle, Institute for Animal Physiology, University of Munich
Mouse anti Chicken CD268 / BAFF-R, clone 2C4, recognizes the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells.
Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 has been shown to cross react with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms.
Mouse anti Chicken CD268 / BAFF-R, clone 2C4 is unique in that it can be used in formalin fixed paraffin embbeded tissues and is therefore of particular use on sections from infection studies.
Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 has been shown to cross react with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms.
Mouse anti Chicken CD268 / BAFF-R, clone 2C4 is unique in that it can be used in formalin fixed paraffin embbeded tissues and is therefore of particular use on sections from infection studies.
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey Quail Duck - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Chicken BAFF-R transfected HEK293 cells
- Approx. Protein Concentrations
- IgG concentration1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD268 / BAFF-R antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Chicken Bu-1A:FITC | MCA2170F | 0.1 mg | F | ||
| Mouse anti Chicken Bu-1a/b | MCA5764 | 0.25 mg | C F IP | ||
| Mouse anti Guinea Fowl Bu-1a/b | MCA5958GA | 0.1 mg | C IP WB |
Product Specific References
Further Reading
-
Reddy, S.K. et al. (2008) The BAFF-Interacting receptors of chickens.
Dev Comp Immunol. 32 (9): 1076-87. -
Kothlow, S. et al. (2010) Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo.
Mol Immunol. 47 (7-8): 1619-28. -
Schneider, K. et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
Int Immunol. 16 (1): 139-48. -
Koskela, K. et al. (2004) Chicken B-cell-activating factor: regulator of B-cell survival in the bursa of fabricius.
Scand J Immunol. 59 (5): 449-57.
