CD268 / BAFF-R antibody | 2C4

Mouse anti Chicken CD268 / BAFF-R

Product Type
Monoclonal Antibody
Clone
2C4
Isotype
IgG1
Specificity
CD268 / BAFF-R

Product Code Applications Pack Size List Price Your Price Qty
MCA6011GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Chicken CD268 / BAFF-R, clone 2C4, recognizes the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells.

Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 cross reacts with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms.

Mouse anti Chicken CD268 / BAFF-R, clone 2C4 is unique in that it can be used in formalin fixed paraffin embedded tissues and is therefore of particular use on sections from infected specimens.

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
Quail
Duck
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Chicken BAFF-R transfected HEK293 cells
Approx. Protein Concentrations
IgG concentration1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100ul

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Further Reading

  1. Reddy, S.K. et al. (2008) The BAFF-Interacting receptors of chickens.
    Dev Comp Immunol. 32 (9): 1076-87.
  2. Kothlow, S. et al. (2010) Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo.
    Mol Immunol. 47 (7-8): 1619-28.
  3. Schneider, K. et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
    Int Immunol. 16 (1): 139-48.
  4. Koskela, K. et al. (2004) Chicken B-cell-activating factor: regulator of B-cell survival in the bursa of fabricius.
    Scand J Immunol. 59 (5): 449-57.

Synonyms
TNFRSF 13C
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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