CD268 / BAFF-R antibody | 2C4
Paraffin sections of bursal tissue were stained with the mouse anti chicken CD268 / BAFF-R antibody, clone 2C4 (2.5 μg/ml) followed by horse anti mouse Ig secondary antibody conjugated to biotin. Visualization was undertaken using a Vector ABC Kit and peroxidase activity was developed using 3.3´ diamino-benzidine-tetrahydro-chloride (DAB).
Image courtesy of Sonja Härtle, Institute for Animal Physiology, University of Munich
Peripheral blood lymphocytes were separated by density centrifugation and first stained with the mouse anti chBAFF-R (clone 2C4) and then with goat anti mouse IgG1 secondary antibody conjugated to FITC, finally blocked with 10% normal mouse serum and followed by staining with the mouse anti chicken Bu-1a/b (clone AV20) conjugated to APC.
Image courtesy of Sonja Härtle, Institute for Animal Physiology, University of Munich
R-Phycoerythrin conjuated Mouse anti Chicken CD268 (BAFF-R) antibody, clone 2C4 (MCA6011GA) conjugated usinf the Lynx-RPE conjugation kit (LNK02) used for the flow cytometric analysis od Mallard peripheral blood cells.
Image caption:
Gating strategy. EDTA-blood was stained with 4A12-APC, anti-chBAFF-R (2C4)-PE, anti-duCD4-FITC, anti-duCD8-PerCp, and anti-2G3-APC-Cy7 in a no-lyse no-wash single-step one-tube procedure and analyzed by flow cytometry. Shown is the gating strategy for the separation of thrombocytes and leukocytes (A), followed by doublet exclusion. (B) Leukocytes were classified as CD4 or CD8 single positive or CD4/CD8 double negative cells. (C) By means of their scatter properties, CD4/CD8 double negative cells were subdivided into granulocytes and monocytes. (D) CD8+ leukocytes were classified into 2C4-negative T cells and 2C4-positive B cells. Initial thrombocyte and leukocyte discrimination (A) was followed by doublet exclusion. Fluorescence-based gates were always followed by FSC/SSC gating. FSC/SSC gates were used to sort purify the respective cell populations (purity, >95%) and prepare cytospin slides followed by DiffQuick staining for thrombocytes (I), CD4+ T cells (II), granulocytes (III), monocytes (IV), CD8+ T cells (V), and B cells (VI).
From: Jax E, Werner E, Müller I, Schaerer B, Kohn M, Olofsson J, Waldenström J, Kraus RHS, Härtle S.
Evaluating Effects of AIV Infection Status on Ducks Using a Flow Cytometry-Based Differential Blood Count.
Microbiol Spectr. 2023 Jun 15:e0435122.
doi: 10.1128/spectrum.04351-22.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD268, clone 2C4 (MCA6011GA) used to label chicken B cells by flow cytometry.
Image caption:
chB6 expression on non-B cells: an IEL preparation was stained with antibodies against chB6 (AV20), TCRγδ(TCR1), and BAFF-R (2C4). Cells were gated for viable, single leukocytes. Gating on chB6+ cells (A) reveals that chB6+ cells consist of three subsets: BAFF-R+/TCRγδ− B cells, BAFF-R−/TCRγδ+ γδ T cells, and so far, uncharacterised BAFF-R−/TCRγδ− cells (B).
From: Citation: Härtle S, Sutton K, Vervelde L and Dalgaard TS (2024)
Delineation of chicken immune markers in the era of omics and multicolor flow cytometry.
Front. Vet. Sci. 11:1385400.
doi: 10.3389/fvets.2024.1385400
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD268 / BAFF-R
- Product Type
- Monoclonal Antibody
- Clone
- 2C4
- Isotype
- IgG1
- Specificity
- CD268 / BAFF-R
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD268 / BAFF-R, clone 2C4, recognizes the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells. Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 cross reacts with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms. Mouse anti Chicken CD268 / BAFF-R, clone 2C4 is unique in that it can be used in formalin fixed paraffin embedded tissues and is therefore of particular use on sections from infected specimens. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey Quail Duck - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken BAFF-R transfected HEK293 cells
- Approx. Protein Concentrations
- IgG concentration1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Chicken Bu-1A:FITC | MCA2170F | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse anti Chicken Bu-1A:FITC | ||||||
| Mouse anti Chicken Bu-1a/b | MCA5764 | C F IP | 0.25 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Chicken Bu-1a/b | ||||||
References for CD268 / BAFF-R antibody
-
Jax, E. et al. (2023) Evaluating Effects of AIV Infection Status on Ducks Using a Flow Cytometry-Based Differential Blood Count.
Microbiol Spectr. : e0435122. -
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 11 [Epub ahead of print].
Further Reading
-
Reddy, S.K. et al. (2008) The BAFF-Interacting receptors of chickens.
Dev Comp Immunol. 32 (9): 1076-87. -
Kothlow, S. et al. (2010) Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo.
Mol Immunol. 47 (7-8): 1619-28. -
Schneider, K. et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
Int Immunol. 16 (1): 139-48. -
Koskela, K. et al. (2004) Chicken B-cell-activating factor: regulator of B-cell survival in the bursa of fabricius.
Scand J Immunol. 59 (5): 449-57.
- Synonyms
- TNFRSF 13C
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