CD184 / CXCR4 antibody | 9D9
Bursa cells were separated by density centrifugation and stained with the anti chCXCR4 antibody, clone 9D9 (MCA6012GA) ) followed by Goat anti Mouse IgG:FITC. The dotted line indicates the isotype / negative control.
Image courtesy of Sonja Härtle, Institute of Animal Physiology, Munich University.
Mouse anti Chicken CD184 / CXCR4
- Product Type
- Monoclonal Antibody
- Clone
- 9D9
- Isotype
- IgG2a
- Specificity
- CD184 / CXCR4
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD184 / CXCR4 clone 9D9, recognizes the chicken homologue of human CD184, also known as CXCR4 (C-X-C Receptor type 4) and fusin. CXCR4 is a receptor for the chemokine, stromal-derived factor-1 (SDF-1), also known as CXCL12. Research has demonstrated that in chicks CXCR4 is expressed by cardiac neural crest cells and together with SDF-1 is involved with the migration of cells and development of the heart. Where CXCR4 function is lost, cell migration is impaired resulting in cell death and misexpression of SDF-1 leading to cardiovascular defects (Escot et al.. 2013). CXCR4 expression is also linked with other development processes including cerebellar development and with vascularization of the gastrointestinal tract. Clone 9D9 provides a useful antibody for studying development biological processes. Bursa cells may be used as a positive control for CXCR4 in flow cytometry. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken CXCR4 (chCXCR4) transfected HEK294 cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Growth Cone | MCA1712 | C P | 2 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse anti Growth Cone | ||||||
References for CD184 / CXCR4 antibody
-
Escot, S. et al. (2013) Misregulation of SDF1-CXCR4 signaling impairs early cardiac neural crest cell migration leading to conotruncal defects.
Circ Res. 113: 505-16. -
Häätle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 23 May [Epub ahead of print].
Further Reading
-
Kaiser, P. et al. (2005) A genomic analysis of chicken cytokines and chemokines.
J Interferon Cytokine Res. 25: 467-84.
- UniProt
- Q9DGI1
MCA6012GA
149223
160996
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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