CD8 Alpha/Beta antibody | vpg9
Mouse anti Cat CD8 Alpha/Beta
- Product Type
- Monoclonal Antibody
- Clone
- vpg9
- Isotype
- IgG1
- Specificity
- CD8 Alpha/Beta
Antibody Quality and Performance Guarantee
| Mouse anti Cat CD8 alpha/beta antibody, clone vpg9 recognizes the feline homolog of human CD8. Clone vpg 9 specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (Shimojima et al. 1998). |
- Target Species
- Cat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Feline thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 lymphocytes in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD8 Alpha/Beta antibody
-
Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system.
P 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency. Oxford University Press, Oxford. -
Callanan, J. et al. (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS.
In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. P. 115-136. -
Shimojima, M. et al. (1998) Characterization of anti-feline CD8 monoclonal antibodies.
Vet Immunol Immunopathol. 61 (1): 17-23. -
Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.
Vet Ther. 8: 229-38. -
Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
J Virol. 84: 3845-56. -
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79. -
Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
PLoS One. 8 (1): e54871. -
Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
J S Afr Vet Assoc. 75 (2): 103-7.
View The Latest Product References
-
Rydzewski, L. et al. (2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.
Vet Immunol Immunopathol. 177: 24-34. -
de Oliveira Medeiros, S. et al. (2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.
J Feline Med Surg. 18 (4): 264-72. -
Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
Vet Comp Oncol. 14 Suppl 1: 40-51. -
O'Brien, T. et al. (2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.
Res Vet Sci. 99: 63-9. -
Sparger, E.E. et al. (2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.
Vet Immunol Immunopathol. 202: 52-62. -
Maeta, N. et al. (2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.
Open Vet J. 9 (2): 147-50. -
Mustaffa-Kamal, F. et al. (2019) Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV).
BMC Vet Res. 15 (1): 165. -
Rütgen, B.C. et al. (2022) Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
J Feline Med Surg. 24 (2): 77-90. -
Cha, S. et al. (2023) Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat
Journal of Veterinary Clinics. 40 (4): 298-302. -
Rütgen, B.C. et al. (2024) Flowcytometric data of intermediate-large cell gastrointestinal lymphoma presenting a gross mass in 32 cats - "let them glow in the flow".
Front Vet Sci. 11: 1378826. -
Wolfesberger, B. et al. (2024) Immunophenotype investigation in feline intestinal non-B-cell lymphoma.
J Comp Pathol. 212: 20-26.
- UniProt
- P41688
- P79336
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0016021 integral to membrane
MCA1347GA
1803
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