CD8 Alpha/Beta antibody | vpg9

Mouse anti Cat CD8 Alpha/Beta

Product Type
Monoclonal Antibody
Clone
vpg9
Isotype
IgG1
Specificity
CD8 Alpha/Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA1347GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Cat CD8 alpha/beta antibody, clone vpg9 recognizes the feline homolog of human CD8. Clone vpg 9 specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (Shimojima et al. 1998).

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Feline thymocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
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loader
Description Mouse IgG1 Negative Control
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD8 Alpha/Beta antibody

  1. Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system.
    P 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency. Oxford University Press, Oxford.
  2. Callanan, J. et al. (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS.
    In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. P. 115-136.
  3. Shimojima, M. et al. (1998) Characterization of anti-feline CD8 monoclonal antibodies.
    Vet Immunol Immunopathol. 61 (1): 17-23.
  4. Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.
    Vet Ther. 8: 229-38.
  5. Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
    J Virol. 84: 3845-56.
  6. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
    J Virol. 81: 9665-79.
  7. Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
    PLoS One. 8 (1): e54871.
  8. Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
    J S Afr Vet Assoc. 75 (2): 103-7.
  9. View The Latest Product References
  10. Rydzewski, L. et al. (2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.
    Vet Immunol Immunopathol. 177: 24-34.
  11. de Oliveira Medeiros, S. et al. (2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.
    J Feline Med Surg. 18 (4): 264-72.
  12. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.
  13. O'Brien, T. et al. (2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.
    Res Vet Sci. 99: 63-9.
  14. Sparger, E.E. et al. (2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.
    Vet Immunol Immunopathol. 202: 52-62.
  15. Maeta, N. et al. (2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.
    Open Vet J. 9 (2): 147-50.
  16. Mustaffa-Kamal, F. et al. (2019) Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV).
    BMC Vet Res. 15 (1): 165.

Flow Cytometry

UniProt
P41688
P79336
Entrez Gene
CD8A
CD8B
GO Terms
GO:0016021 integral to membrane
View more products with CD8 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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