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CD8 Alpha/Beta antibody | vpg9

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Anti Cat CD8 Alpha/Beta Antibody, clone vpg9 gallery image 1
Cat lymphocytes stained with Mouse anti Cat CD8:Alpha/Beta (MCA1347GA) followed by Goat anti Mouse IgG:FITC (STAR117F); dual stained with Mouse anti Cat CD4:RPE

Mouse anti Cat CD8 Alpha/Beta

Mouse anti Cat CD8 alpha/beta:RPE

Product Type
Monoclonal Antibody
Clone
vpg9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1347GA
Datasheet
C F
SDS
0.1 mg loader

MCA1347PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Cat CD8 alpha/beta antibody, clone vpg9 recognizes the feline homolog of human CD8. Clone vpg 9 specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (Shimojima et al. 1998).

Product Details

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Feline thymocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P41688
P79336
Entrez Gene
CD8A
CD8B
GO Terms
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of CD8 Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD8 Alpha/Beta antibody

  1. Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system.
    P 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency. Oxford University Press, Oxford.
  2. Callanan, J. et al. (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS.
    In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. P. 115-136.
  3. Shimojima, M. et al. (1998) Characterization of anti-feline CD8 monoclonal antibodies.
    Vet Immunol Immunopathol. 61 (1): 17-23.
  4. Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.
    Vet Ther. 8: 229-38.
  5. Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
    J Virol. 84: 3845-56.
  6. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
    J Virol. 81: 9665-79.
  7. Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
    PLoS One. 8 (1): e54871.
  8. Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
    J S Afr Vet Assoc. 75 (2): 103-7.
  9. Rydzewski, L. et al. (2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.
    Vet Immunol Immunopathol. 177: 24-34.
  10. de Oliveira Medeiros, S. et al. (2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.
    J Feline Med Surg. 18 (4): 264-72.
  11. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.
  12. O'Brien, T. et al. (2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.
    Res Vet Sci. 99: 63-9.
  13. Sparger, E.E. et al. (2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.
    Vet Immunol Immunopathol. 202: 52-62.
  14. Maeta, N. et al. (2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.
    Open Vet J. 9 (2): 147-50.
  15. Mustaffa-Kamal, F. et al. (2019) Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV).
    BMC Vet Res. 15 (1): 165.

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