CD8 Alpha/Beta antibody | vpg9
Product Details
- Target Species
- Cat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Feline thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P41688
- P79336
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of CD8 Alpha/Beta antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
![]() |
|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
![]() |
|
Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
![]() |
|
Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
![]() |
Product Specific References
References for CD8 Alpha/Beta antibody
-
Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system.
P 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency. Oxford University Press, Oxford. -
Callanan, J. et al. (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS.
In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. P. 115-136. -
Shimojima, M. et al. (1998) Characterization of anti-feline CD8 monoclonal antibodies.
Vet Immunol Immunopathol. 61 (1): 17-23. -
Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in
cats.
Vet Ther. 8: 229-38. -
Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
J Virol. 84: 3845-56. -
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79. -
Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
PLoS One. 8 (1): e54871. -
Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
J S Afr Vet Assoc. 75 (2): 103-7. -
Rydzewski, L. et al. (2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.
Vet Immunol Immunopathol. 177: 24-34. -
de Oliveira Medeiros, S. et al. (2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.
J Feline Med Surg. 18 (4): 264-72. -
Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
Vet Comp Oncol. 14 Suppl 1: 40-51. -
O'Brien, T. et al. (2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.
Res Vet Sci. 99: 63-9. -
Sparger, E.E. et al. (2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.
Vet Immunol Immunopathol. 202: 52-62. -
Maeta, N. et al. (2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.
Open Vet J. 9 (2): 147-50.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra