Chlamydia LPS antibody | CF 6J12
Mouse anti Chlamydia LPS antibody, clone CF 6J12 used for the detection of Chlamydia LPS in aortic lesions of Chlamydia infected mive by immunofluorescence.
Image caption:
1a. Agarose gel electrophoresis of PCR products (first stage, lanes 2–6; second stage, lanes 8–12). Lanes 2 and 3 using genomic DNA as a template from two Apobtm2SgyLdlrtm1Her/J mice infected with Cpn bacteria; lanes 5 and 6 using genomic DNA from two non-infected Apobtm2SgyLdlrtm1Her/J mice; lanes 8, 9, 11 and 12 using first-stage PCR products 2, 3, 5 and 6 as a template, respectively. Lanes 1 and 7 show the Phi174 DNA/HaeIII maker (Promega). 1b. Chlamydia LPS antibody (MCA 2718, Bio-Rad) stained lesion sites in aortic sinus. Chlamydia LPS antibody (MCA 2718, Bio-Rad) was used as the first antibody (10 mg/ml), anti-mouse IgG-FITC, developed in sheep, was used as a second antibody. Green represents Chlamydia LPS and blue represents cell nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Lab, Peterborogh, UK) [N = 6 mice].
From: Xia M, Chen D, Endresz V, Faludi I, Szabo A, Gonczol E, et al. (2013) Immunization of Chlamydia pneumoniae (Cpn)-Infected Apobtm2SgyLdlrtm1Her/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development. PLoS ONE 8(12): e81056.
Mouse anti Chlamydia LPS antibody, clone CF 6J12 recognizes a genus specific epitope within Chlamydia lipopolysaccharide (LPS).
LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella minnesota or Neisseria gonorrhoeae.
LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella minnesota or Neisseria gonorrhoeae.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Elementary bodies from C. trachomatis strain SAF2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0/U mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Chlamydia LPS antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Chlamydia LPS antibody
-
Thornley, M.J. et al. (1985) Properties of monoclonal antibodies to the genus-specific antigen of Chlamydia and their use for antigen detection by reverse passive haemagglutination.
J Gen Microbiol. 131 (1): 7-15. -
Xia M et al. (2013) Immunization of Chlamydia pneumoniae (Cpn)-infected Apob(tm2Sgy)Ldlr(tm1Her)/J mice with a combined peptide of Cpn significantly reduces atherosclerotic lesion development.
PLoS One. 8 (12): e81056. -
Campbell, S. et al. (1994) Lipopolysaccharide in cells infected by Chlamydia trachomatis.
Microbiology.140: 1995-2002.
Further Reading
-
Ingalls, R.R. et al. (1995) The inflammatory cytokine response to Chlamydia trachomatis infection is endotoxin mediated.
Infect Immun. 63 (8): 3125-30.
