Chlamydia LPS antibody | CF 6J12
Mouse anti Chlamydia LPS antibody, clone CF 6J12 (MCA2718) used for the detection of Chlamydia LPS in aortic lesions of Chlamydia infected mice by immunofluorescence.
Image caption:
1a. Agarose gel electrophoresis of PCR products (first stage, lanes 2–6; second stage, lanes 8–12). Lanes 2 and 3 using genomic DNA as a template from two Apobtm2SgyLdlrtm1Her/J mice infected with Cpn bacteria; lanes 5 and 6 using genomic DNA from two non-infected Apobtm2SgyLdlrtm1Her/J mice; lanes 8, 9, 11 and 12 using first-stage PCR products 2, 3, 5 and 6 as a template, respectively. Lanes 1 and 7 show the Phi174 DNA/HaeIII maker (Promega). 1b. Chlamydia LPS antibody (MCA 2718, Bio-Rad) stained lesion sites in aortic sinus. Chlamydia LPS antibody (MCA 2718, Bio-Rad) was used as the first antibody (10 mg/ml), anti-mouse IgG-FITC, developed in sheep, was used as a second antibody. Green represents Chlamydia LPS and blue represents cell nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Lab, Peterborogh, UK) [N = 6 mice].
From: Xia M, Chen D, Endresz V, Faludi I, Szabo A, Gonczol E, et al. (2013)
Immunization of Chlamydia pneumoniae (Cpn)-Infected Apobtm2SgyLdlrtm1Her/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development.
PLoS ONE 8(12): e81056.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Chlamydia LPS antibody, clone CF 6J12 (MCA2718) used to identify chlamydial inclusions in Hep-2 cells by immunofluorescence.
Image caption:
G3BP1 is accumulated around the chlamydial inclusion in Cp. psittaci infected Hep-2 cells.
Hep-2 cells were infected with Cp. psittaci at a MOI of 3, and at 48 h postinfection the cells were fixed and stained with anti-chlamydial LPS (red), anti-G3BP1 (green) antibodies and DAPI (blue, nuclei), and viewed under a fluorescence (1–3) or a confocal laser-scanning microscope (4–6). Non-infected cells served as control (1–3). The anti-G3BP1 (1 and 4) and anti-LPS (5) or DAPI (2) signals were merged (3 and 6). A fraction of endogenous G3BP1 is concentrated around chlamydial inclusions (6). Bar = 20 μm.
From: Borth N, Litsche K, Franke C, Sachse K, Saluz HP, Hänel F (2011)
Functional Interaction between Type III-Secreted Protein IncA of Chlamydophila psittaci and Human G3BP1.
PLoS ONE 6(1): e16692.
doi: 10.1371/journal.pone.0016692
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chlamydia LPS antibody, clone CF 6J12 (MCA2718) used to evaluate Chlamydial LPS expression in Hep-2 cells by western blotting.
Image caption:
Infection of Hep-2 cells with Cp. psittaci leads to a decrease in c-Myc protein concentration.
Hep-2 cells were infected with Cp. psittaci at a MOI of 3, with heat-inactivated Cp. psittaci or chloramphenicol-treated after infection as controls, and at different hours postinfection (hpi) cells were lysed for RNA or protein analysis. (B) Immunoblots of whole cell lysates of Cp. psittaci infected (middle column), infected and chloramphenicol-treated (right) and control (left) cells probed with anti-LPS, anti-c-Myc and anti-β-actin antibodies. Bar diagrams below the immunoblots represent a quantification of c-Myc protein related to β-actin protein concentration. Experiments were performed three times.
From: Borth N, Litsche K, Franke C, Sachse K, Saluz HP, Hänel F (2011)
Functional Interaction between Type III-Secreted Protein IncA of Chlamydophila psittaci and Human G3BP1.
PLoS ONE 6(1): e16692.
doi: 10.1371/journal.pone.0016692
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chlamydia LPS antibody, clone CF 6J12 (MCA2178) used to evaluate LPS expression in patient biopsies by western blotting.
Image caption:
Western blot analysis of small bowel biopsies from 4 IBS patients against mouse immune sera to LPS and MOMP, respectively. 1-4 = IBS patients, ++ = HeLa cells infected with C. trachomatis (10 μl), + = HeLa cells infected with C. trachomatis (5 μl), - = non-infected HeLa cells.
From: Dlugosz A, Törnblom H, Mohammadian G, Morgan G, Veress B, Edvinsson B, Sandström G, Lindberg G
Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the small bowel in patients with severe irritable bowel syndrome.
BMC Gastroenterol. 2010 Feb 16;10:19.
doi: 10.1186/1471-230X-10-19.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chlamydia LPS antibody, clone CF 6J12 (MCA2718) used to demonstrate Chlamydia LPS expression in infected mouse tissues by immunofluorescence.
Image caption:
Chlamydia LPS antibody (MCA 2718, AbD serotec) stained lesion sites in aortic sinus. Chlamydia LPS antibody (MCA 2718, AbD serotec) was used as the first antibody (10 mg/ml), anti-mouse IgG-FITC, developed in sheep, was used as a second antibody. Green represents Chlamydia LPS and blue represents cell nnucleuses stained with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Lab, Peterborogh, UK) [N = 6 mice].
From: Xia M, Chen D, Endresz V, Faludi I, Szabo A, Gonczol E, Kakkar V, Lu X.
Immunization of Chlamydia pneumoniae (Cpn)-infected Apob(tm2Sgy)Ldlr(tm1Her)/J mice with a combined peptide of Cpn significantly reduces atherosclerotic lesion development.
PLoS One. 2013 Dec 13;8(12):e81056.
doi: 10.1371/journal.pone.0081056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chlamydia LPS antibody, clone CF 6J12 recognizes a genus specific epitope within Chlamydia lipopolysaccharide (LPS).
LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella minnesota or Neisseria gonorrhoeae.
LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella minnesota or Neisseria gonorrhoeae.
Product Details
- Target Species
- Bacterial
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chlamydophila sp. - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Elementary bodies from C. trachomatis strain SAF2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0/U mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Chlamydia LPS antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Chlamydia LPS antibody
-
Thornley, M.J. et al. (1985) Properties of monoclonal antibodies to the genus-specific antigen of Chlamydia and their use for antigen detection by reverse passive haemagglutination.
J Gen Microbiol. 131 (1): 7-15. -
Xia M et al. (2013) Immunization of Chlamydia pneumoniae (Cpn)-infected Apob(tm2Sgy)Ldlr(tm1Her)/J mice with a combined peptide of Cpn significantly reduces atherosclerotic lesion development.
PLoS One. 8 (12): e81056. -
Campbell, S. et al. (1994) Lipopolysaccharide in cells infected by Chlamydia trachomatis.
Microbiology.140: 1995-2002. -
Xia, M. et al. (2013) Immunization of Chlamydia pneumoniae. (Cpn)-infected Apob(tm2Sgy)Ldlr(tm1Her)/J mice with a combined peptide of Cpn significantly reduces atherosclerotic lesion development.
PLoS One. 8 (12): e81056. -
Borth, N. et al. (2011) Functional interaction between type III-secreted protein IncA of Chlamydophila psittaci. and human G3BP1.
PLoS One. 6 (1): e16692. -
Dlugosz, A. et al. (2010) Chlamydia trachomatis. antigens in enteroendocrine cells and macrophages of the small bowel in patients with severe irritable bowel syndrome.
BMC Gastroenterol. 10: 19. -
Lantos, I. et al. (2018) Chlamydia pneumoniae Infection Exacerbates Atherosclerosis in ApoB100only/LDLR-/- Mouse Strain.
Biomed Res Int. 2018: 8325915. -
Mosolygó, T. et al. (2019) Selenocompounds as Novel Antibacterial Agents and Bacterial Efflux Pump Inhibitors.
Molecules. 24(8):1487.
Further Reading
-
Ingalls, R.R. et al. (1995) The inflammatory cytokine response to Chlamydia trachomatis infection is endotoxin mediated.
Infect Immun. 63 (8): 3125-30.
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