Anthrax antibody | C3
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Mouse anti Anthrax Protective Antigen
- Product Type
- Monoclonal Antibody
- Clone
- C3
- Isotype
- IgG1
- Specificity
- Anthrax
- Region
- PROTECTIVE ANTIGEN
Antibody Quality and 
Unrivaled 
| Mouse anti Anthrax Protective Antigen antibody, clone C3 recognizes the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol. Mouse anti Anthrax Protective Antigen antibody, clone C3 does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensis or Toxoplasma gondii. The antibody recognizes both the 83kDa and 63kDa truncated form of PA. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Highly purified protective antigen of Bacillus anthracis.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Anthrax antibody
-
Barth H, et al (2004) Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins.
Microbiol Mol Biol Rev. 68:373-402
- RRID
- AB_1100460
- UniProt
- P13423
- Entrez Gene
- pxo1_110
- GO Terms
- GO:0005515 protein binding
- GO:0046872 metal ion binding
- GO:0005615 extracellular space
- GO:0009405 pathogenesis
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