ALIX antibody | 3A9

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Mouse anti ALIX

Product Type
Monoclonal Antibody
Clone
3A9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2493 E IF IP P WB 0.1 mg
Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting (Kim et al. 2005).

ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 (Mahul-Mellier et al. 2006) but has subsequently shown to be involved in endocytosis (Matsuo et al. 2004), viral budding (Irie et al. 2008) and actin cytoskeletal assembly (Pan et al. 2006).

Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting (Sun et al. 2016) and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus (Romancino et al. 2013).

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Xenopus
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Alix-GST fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 murine myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q8WUM4 Related reagents
Entrez Gene
PDCD6IP Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005815 microtubule organizing center
GO:0007049 cell cycle
GO:0015031 protein transport
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0048306 calcium-dependent protein binding
GO:0051301 cell division
Regulatory
For research purposes only

Applications of ALIX antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections.

Application Based External Images

Functional Assays

Immuno-electron Microscopy

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

Source Reference

  1. Pan, S. et al. (2006) Involvement of the conserved adaptor protein Alix in actin cytoskeleton assembly.
    J Biol Chem. 281 (45): 34640-50.

References for ALIX antibody

  1. Pan, S. et al. (2008) Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly.
    EMBO J. 27: 2077-90.
  2. Dangaj, D. et al. (2011) Mannose Receptor (MR) Engagement by Mesothelin GPI Anchor Polarizes Tumor-Associated Macrophages and Is Blocked by Anti-MR Human Recombinant Antibody.
    PLoS One. 6: e28386.
  3. Liu, W. et al. (2012) Galectin-3 regulates intracellular trafficking of EGFR through Alix and promotes keratinocyte migration.
    J Invest Dermatol. 132 (12): 2828-37.
  4. Zhou, X. et al. (2008) The HIV-1 p6/EIAV p9 docking site in Alix is autoinhibited as revealed by a conformation-sensitive anti-Alix monoclonal antibody.
    Biochem J. 414: 215-20.
  5. Pusic, A.D. & Kraig, R.P. (2014) Youth and environmental enrichment generate serum exosomes containing miR-219 that promote CNS myelination.
    Glia. 62 (2): 284-99.
  6. Chen, H.Y. et al. (2009) Galectin-3 negatively regulates TCR-mediated CD4+ T-cell activation at the immunological synapse.
    Proc Natl Acad Sci U S A. 106 (34): 14496-501.
  7. Pusic AD et al. (2014) IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.
    J Neuroimmunol. 266 (1-2): 12-23.
  8. Pusic, A.D. & Kraig, R.P. (2015) Phasic Treatment with Interferon Gamma Stimulates Release of Exosomes that Protect Against Spreading Depression.
    J Interferon Cytokine Res. 35 (10): 795-807.
  9. Sun, S. et al. (2016) Phosphorylation-Dependent Activation of the ESCRT Function of ALIX in Cytokinetic Abscission and Retroviral Budding.
    Dev Cell. 36 (3): 331-43.
  10. Sun, S. et al. (2015) ALG-2 activates the MVB sorting function of ALIX through relieving its intramolecular interaction.
    Cell Discov. 1: 15018.
  11. Feng, Z. et al. (2007) Alternative polyadenylation produces two major transcripts of Alix.
    Arch Biochem Biophys. 465 (2): 328-35.