Sulfatase 2 Antibody | 2B4

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Sulfatase 2 Antibody | 2B4 gallery image 1

Paraffin embedded HEK293 cells transfected with SULF2 stained with Mouse anti Human Sulfatase 2 C-terminal (MCA5692T)

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  • Mouse anti Human Sulfatase 2 (C-Terminal)
  • Mouse anti Human Sulfatase 2 (C-Terminal)
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  • Product Type
    Monoclonal Antibody
  • Clone
    2B4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5692GAE, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5692GA
    MCA5692TE, P *, WBdatasheet pdfdatasheet pdf25 µg
    MCA5692T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Studies have linked Sulf-2 with several cancers, including breast, hepatocellular, and pancreatic cancer, and recently with non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010).

      Mature human Sulf-2 is a heterodimer consisting of a 50kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75kDa N-terminal subunit which contains the catalytic centre.

      Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
    • Preservative Stabilisers
      0.09% Sodium Azide.
      0.09% Sodium Azide.
    • Immunogen
      Recombinant human Sulf-2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      endoplasmic reticulum
      Golgi stack
      cell surface
      heparan sulfate proteoglycan metabolic process
      extracellular space
      calcium ion binding
      arylsulfatase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
    • Histology Positive Control Tissue
      Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Mouse anti Human sulfatase 2 antibody, clone 2B4 detects a major band of ~50kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.
    • Western Blotting
      Mouse anti Human sulfatase 2 antibody, clone 2B4 detects a major band of ~50kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.
    • Instructions For Use
    • Instructions For Use

    Additional Sulfatase 2 Antibody Formats

    Formats Clone Applications Sizes available
    Sulfatase 2 Antibody : Purified 2B4 E, P *, WB 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
              Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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