Sphingosine 1- Phosphate Receptor 1 Antibody | 2B9

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Sphingosine 1- Phosphate Receptor 1 Antibody | 2B9 gallery image 1

Flow cytometric staining of human S1P1 on CHO cells stably transfected with rHuS1P1-GFP (red) or CHO-K1 cells as a negative control (black) detected by GFP fluorescence [d] or with Mouse anti Human Sphingosine 1-phosphate receptor 1 [d'] (MCA3960GA)

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Sphingosine 1- Phosphate Receptor 1 Antibody | 2B9 gallery image 2

Immunofluorescence staining of human S1P1 with Mouse anti Human Sphingosine 1-phosphate receptor 1 on Huvec cells (MCA5960GA) [bottom] or with no primary antibody as a negative control [top)], followed by an anti Mouse IgG secondary conjugated to AlexaFluor488®

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Sphingosine 1- Phosphate Receptor 1 Antibody | 2B9 gallery image 3

Immunofluorescent of CHO cells stably transfected with human S1P1-GFP or CHO-K1 cells using GFP fluorescence [a,b,i,j] or using Mouse anti Human Sphingosine 1-phosphate receptor 1 (MCA5960GA) [c,d], no primary control [k,l]. Merged images [g,h,o,p] demonstrate co-localization of GFP-S1P1 [h]

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Sphingosine 1- Phosphate Receptor 1 Antibody | 2B9 gallery image 4

Immunoprecipitation of human S1P1 from CHO cells stably transfected with human S1P1-GFP
lane 1: CH0-S1P1-GFP
Lane 2: Control with no primary antibody
Lane 3: CHO-S1P1-GFP solubized with NP-40
Lane 4: Immunoprecipitated sample using Mouse anti Human Sphingosine 1-phosphate receptor 1(MCA5960GA).
Samples were probed using a Rabbit anti GFP coupled to Horseradish peroxidase and visualized using chemiluminescence

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Sphingosine 1- Phosphate Receptor 1 Antibody | 2B9 gallery image 5

Western blot analysis of human S1P1 expression on CHO and Huvec cell lines using Mouse anti Human Sphingosine 1-phosphate receptor 1 (MCA5960GA)
Lane 1: CHO-K1 cell membranes (negative control)
Lane 2: CHO-S1P1-GFP stable transfectant
Lane 3: Huvec cell membranes
The GFP-S1P1 transfectant migrates at an apparent MW of ~70kDa whilst the native S1P1 expressed on HUVEC cells migrates at an apparent MW of ~43kDa

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  • Mouse anti Human Sphingosine 1- Phosphate Receptor 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2B9
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5960GAF, IF, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5960GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Sphingosine 1-Phosphate Receptor 1, clone 2B9 recognizes S1P1, otherwise known as CD363/EDG-1, a bioactive lysophospholipid and inducible member of the G-protein coupled receptor superfamily (GPCR) which plays an important role in a variety of cellular processes.

      During inflammatory diseases such as atherosclerosis the phenotype switching of activated macrophages is influenced by the presence of particular cytokines within the microenvironment. These cells have been characterized as classically activated M1 macrophages, in response to pro-inflammatory molecules such as interferon gamma and lipopolysaccharide, or as alternatively activated M2 macrophages, in response to anti-inflammatory cytokines such as interleukin-4. Activation of S1P1 through its ligand S1P (Sphingosine 1-Phosphate) has been identified as an important promoter of anti-inflammatory M2 macrophages (Hughes et al. 2008).

      Apoptotic cells can also influence the pro-versus anti-inflammatory macrophage response, and S1P acts as a positive regulator for anti-apoptotic/pro-survival responses by binding to S1P1. Growing evidence suggests that the expression of S1P1 on macrophages may convey protection against apoptosis, at least in part, having implications for the pathogenesis of inflammatory diseases and cancer.

      Deviation in sphingosine 1-phosphate (S1P) signaling is important in many types of cancer. Analysis of S1P1 and SK1 receptors during the development of tamoxifen resistance in Estrogen Receptor (ER) breast cancer patients concurs that the expression levels of S1P1 correlates with tumor size and Progesterone Receptor (PR) status and that high S1P1 membrane expression is associated with shorter time to recurrence.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      plasma membrane
      cell adhesion
      integral to membrane
      lysosphingolipid and lysophosphatidic acid receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Immunofluorescence1/500
      Immunoprecipitation1/300
      Western Blotting1/5000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Sphingosine 1- Phosphate Receptor 1 Antibody Formats

    Formats Clone Applications Sizes available
    Sphingosine 1- Phosphate Receptor 1 Antibody : Purified 2B9 F, IF, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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