RelB Antibody | 17.3

RelB Antibody | 17.3 gallery image 1

Western blot analysis of RelB was performed by loading 20 ug of THP-1 (lane1), Daudi (lane2) and Raji (lane3) cell lysate on a gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skimmed milk for 1 hour at room temperature. RelB was detected at ~ 63 kDa using Mouse anti RelB (MCA6037) at 1-2 ug/ml in 5 % skimmed milk at 4°C overnight on a rocking platform. A goat anti-mouse HRP conjugated secondary antibody was used for detection

RelB Antibody | 17.3 gallery image 2

Immunofluorescence analysis of RelB was performed on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Mouse anti RelB antibody (MCA6037) at 1.0 ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with an Alexa Fluor® 488 conjugated goat anti-mouse IgG secondary antibody for 45 minutes at room temperature (Panel A: green). Nuclei (Panel B: blue) were counterstained with DAPI and F-actin (Panel C: red) with rhodamine phalloidin. Panel D is a merged image showing nuclear localization. Panel E is a no primary antibody control. The images were captured at 60x magnification

  • RelB Antibody | 17.3 thumbnail image 1
  • RelB Antibody | 17.3 thumbnail image 2
  • Mouse anti RelB
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6037IC, IF, IP, P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti-RelB antibody, clone 17.3 recognizes the transcription factor RelB, also known as I-Rel. RelB is encoded by the v-rel avian reticuloendotheliosis viral oncogene homolog B gene and is a critical component of the NF-kappa-B pathway.

      Upon cleavage of the NF-kappa-B p105 and p100 subunits into the NF-kappa-B p50 and p52 subunits, RelB forms RelB-p50 and RelB-p52 heterodimers. These dimers act as activators of transcription by binding to kappaB sites present on DNA (Oeckinghaus and Ghosh 2009). RelB-p50 and RelB-p52 binding induces the expression of genes mediating immune responses, inflammation and apoptosis (Jing and Lee 2014).

      Misregulation of the NF-kappa-B pathway has been implicated in a variety of disease states including autoimmunity and cancer. As the NF-kappa-B pathway has been demonstrated to be constitutively active in a large group of tumors, developing NF-kappa-B inhibitors has emerged as a new cancer therapy approach (Chaturverdi et al. 2011 and Baud and Karin et al. 2009).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Recombinant protein derived from C-terminal region of human RelB protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      sequence-specific DNA binding transcription factor activity
      transcription corepressor activity
      protein binding
      regulation of transcription, DNA-dependent
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunocytochemistry1 ug/ml
      Immunofluorescence1 ug/ml
      Immunohistology - Paraffin1/101/50
      Western Blotting1 - 2 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse anti RelB antibody recognizes a band of approximately 63 kDa in THP-1 cell lysate, Daudi cell lysate, and Raji cell lysate
    • Instructions For Use

    Additional RelB Antibody Formats

    Formats Clone Applications Sizes available
    RelB Antibody : Purified 17.3 IC, IF, IP, P, WB 50 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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