Nestin Antibody | 10C2

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Nestin Antibody | 10C2 gallery image 1

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Overview of representative punches of control and tumor tissue samples stained with Hematoxylin/Eosin (H/E) or immunostained for the 24 specific proteins grouped by categories.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

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Nestin Antibody | 10C2 gallery image 2

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of fetal, epileptic and gliotic tissues immunostained for nestin, SPARC and IDH1(R132H). IDH1(R132H) was not expressed in these control brain samples, whereas nestin was highly expressed in fetal tissue, not in epilepsy (except one single cell shown in inlet), but was present in reactive glial cells in gliotic tissue (black arrows) and in microvessels (white arrows). SPARC was expressed in reactive glial cells in gliotic tissue (black arrows), as well as in rare cells in epileptic tissue (black arrows), in addition to some vascular cells (white arrows). Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
Nestin Antibody | 10C2 gallery image 3

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of fetal, epileptic and gliotic tissues immunostained for nestin, SPARC and IDH1(R132H). IDH1(R132H) was not expressed in these control brain samples, whereas nestin was highly expressed in fetal tissue, not in epilepsy (except one single cell shown in inlet), but was present in reactive glial cells in gliotic tissue (black arrows) and in microvessels (white arrows). SPARC was expressed in reactive glial cells in gliotic tissue (black arrows), as well as in rare cells in epileptic tissue (black arrows), in addition to some vascular cells (white arrows). Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
Nestin Antibody | 10C2 gallery image 4

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of two cases of low grade astrocytomas immunostained for nestin, SPARC and IDH1(R132H). Case 1 expressed the mutated IDH1 protein, whereas case 2 was clearly IDH1(R132H)-negative. Both cases were nestin and SPARC immunopositive. The center (c) and periphery (p) of the corresponding tumors are depicted for each case. Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
Nestin Antibody | 10C2 gallery image 5

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of two cases of anaplastic astrocytoma immunostained for nestin, SPARC and IDH1(R132H). Case 1 was positive for all three markers, whereas case 2 was IDH1(R132H) negative and low for SPARC expression. For both cases, the center (c) and periphery (p) of the tumors are depicted. Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
Nestin Antibody | 10C2 gallery image 6

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of tumors in pGBM and sGBM immunostained for nestin, SPARC and IDH1(R132H). The case of sGBM was positive for all three markers, whereas pGBM was IDH1(R132H) negative. The center (c) and periphery (p) of the tumors are shown. Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
Nestin Antibody | 10C2 gallery image 7

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of the tumor centers of two cases of sGBM with low SPARC immunostaining. The two cases (case 1 and 2) showed high cellularity, with strong nuclear expression of PCNA and p53 in almost all cells, in addition to the expression of IDH1(R132H). Case 1 showed low number of nestin-positive cells, whereas case 2 revealed strong nestin immunostaining in all cells at the “hot spots” indicated by black arrowheads. Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
Nestin Antibody | 10C2 gallery image 8

Published customer image:
Mouse anti Human Nestin antibody, clone 10 C2 used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of medulloblastoma and metastasis immunostained for nestin, SPARC and IDH1(R132H). Nestin was mainly observed expressed by tumor vasculature and some rare tumor cells. SPARC expression was strong in medulloblastoma cells and the cells constituting the rosette in the metastasis. No IDH1(R132H) staining was observed in these two cases. Original magnification x400. Scale bar: 100 µm.

From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.

Enlarge
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  • Mouse anti Human Nestin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    10C2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1610C, P, WBdatasheet pdfdatasheet pdf0.1 mg
    OBT1610
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Nestin antibody, clone 10C2 recognizes nestin, a large intermediate filament protein originally identified by Hockfield and McKay (1985). Nestin is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      Ratno
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      embryonic camera-type eye development
      positive regulation of neural precursor cell proliferation
      cytoplasm
      intermediate filament
      positive regulation of intermediate filament depolymerization
      structural molecule activity
      brain development
      intermediate filament binding
      negative regulation of apoptosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin1/501/200
      Western Blotting1/5001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Suggested positive control: WI-38 (ATCC# CCL75) or U251 cell lysate.
    • Instructions For Use

    Additional Nestin Antibody Formats

    Formats Clone Applications Sizes available
    Nestin Antibody : Purified 10C2 C, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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