HLA G Antibody | MEM-G/9

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HLA G Antibody | MEM-G/9 gallery image 1

Published customer image:
Mouse anti Human HLA-G antibody, clone MEM-G/9 used for the evaluation of HLA-G expression on transfected cells by flow cytometry.
Image caption:
MiR-148a and miR-152 down-regulate HLA-G expression and reduce LILRB1 binding. 721.221/HLA-G (G variant, A, or C variant, B) were transduced with the control miRNA, miR-148a or miR-152. The various cells were then stained with anti HLA-G mAb and analyzed by FACS. One out of three representative experiments is shown. (C) FACS histograms re HLA-G staining of 721.221/HLA-G cells not expressing the 3′UTR of HLA-G. One out of three representative experiments is shown. (D–E) LILRB1-Ig staining of 721.221/HLA-G (G variant, D, or C variant, E) and of 21.221/HLA-G cells not expressing the 3′UTR of HLA-G (F) transduced with control miRNA, with miR-148a or with miR-152. Black histogram: cells transduced with a control miRNA. Dark grey histogram: cells transduced with miR-148a. Light grey histogram: cells transduced with miR-152. One out of three representative experiments is shown. (G) Quantitative real-time PCR analysis of HLA-G mRNA levels in 721.221/HLA-G cells (G variant), presented relative to hUBC. One out of three representative experiments is shown.

From: Manaster I, Goldman-Wohl D, Greenfield C, Nachmani D, Tsukerman P, Hamani Y, et al. (2012) MiRNA-Mediated Control of HLA-G Expression and Function.
PLoS ONE 7(3): e33395.

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HLA G Antibody | MEM-G/9 gallery image 2

Published customer image:
Mouse anti HLA-G antibody, clone MEM-G/9 used for the evaluation of cuirculating HLA-G in Primary Antiphospholipid Syndrome and control patients by ELISA.
Image caption:
HLA-G serum levels in 44 PAPS patients and 43 healthy controls, and in PAPS patients with and without heparin.

From: de Carvalho JF, de Oliveira RM, Rodrigues CE, Glezer A, Bonfá E, Pereira RM. Heparin increases HLA-G levels in primary antiphospholipid syndrome.
Clin Dev Immunol. 2012;2012:232390.

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HLA G Antibody | MEM-G/9 gallery image 3

Published customer image:
FITC conjugated Mouse anti Human HLA-G antibody, clone MEM-G/9 (MCA2044F) used for the demonstration of HLA-G expression on placental trophoblasts by immunofluorescence
Image caption:
Dual immunofluorescence using HLA-G (an extravillous trophoblast marker) and anti-M30 cytodeath reveals that trophoblast apoptosis is markedly increased in spontaneous abortions.

From: Guenther S, Vrekoussis T, Heublein S, Bayer B, Anz D, Knabl J, Navrozoglou I, Dian D, Friese K, Makrigiannakis A, Jeschke U. Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis.
Int J Mol Sci. 2012;13(7):9069-80.

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HLA G Antibody | MEM-G/9 gallery image 4

Published customer image:
FITC conjugated Mouse anti Human HLA-G antibody, clone MEM-G/9 used for the identification of HLA-G expressing trophoblast cells by flow cytometry.
Image caption:
The IL-2 receptor β subunit is up-regulated on trophoblast cells as they differentiate from villous to extravillous phenotype. Upregulation of IL-2Rβ expression was validated by flow cytometry (A–C) and immunohistochemistry (D). Preparations of placental cells from normal first trimester pregnancies were gated on scatter, leukocytes excluded by CD45 labelling (gate R2) and subunits of the IL-15 receptor complex stained on villous (EGF-R+) or extravillous (HLA-G+) trophoblast cells (A). Dot plots are shown of IL-2Rβ subunit staining villous (B) and extravillous trophoblast (C). Shown here are cells from the same placenta which is representative of 3 pregnancies analysed. The IL-2Rβ was also localised histologically in sections of implantation site from the first trimester (D). The low power plan stained with cytokeratin shows villous mesenchyme (top left) and a column of EVT (bottom right) developing from placental villi (centre). Higher power pictures show strong IL-2Rβ staining detected on extravillous trophoblast migrating away from the villi, whereas staining was negligible on villous trophoblast and villous mesenchymal cells.

From: Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A. Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
Placenta. 2011 Jan;32(1):33-43.

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  • Mouse anti Human HLA G:FITC
  • Mouse anti Human HLA G
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-G/9
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2044FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2044F
    MCA2044C *, E, F, IFdatasheet pdfdatasheet pdf0.2 mg
    MCA2044
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human HLA G antibody, clone MEM-G/9 recognizes human HLA-G, a non-classical major histocompatibility complex (MHC) molecule. HLA-G expression is restricted to trophoblast cells and some medullary thymic epithelial cells. Several isoforms of the HLA-G molecule exist, which include the membrane bound isoforms HLA-G1 – G4 and soluble isoforms HLA-G5 – G7. Clone MEM-G/9 specifically recognizes surface expressed native HLA-G1, when associated with beta 2 microglobulin, but not does recognize the isoforms HLA-G2, G3 and G4. CMouse anti Human HLA G antibody, clone MEM-G/9 has also been reported to recognize the soluble isoform HLA-G5.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Recombinant human HLA-G refolded with beta 2 microglobulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with myeloma cells.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cellular defense response
      MHC class I protein complex
      immune response
      regulation of immune response
      type I interferon-mediated signaling pathway
      antigen processing and presentation of peptide antigen via MHC class I
      MHC class I receptor activity
      integral to membrane
      interferon-gamma-mediated signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Paraffin
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional HLA G Antibody Formats

    Formats Clone Applications Sizes available
    HLA G Antibody : Purified MEM-G/9 C *, E, F, IF 0.2 mg
    HLA G Antibody : FITC MEM-G/9 F 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
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              References

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