Estrogen Receptor Beta 2 Antibody | 57/3

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Estrogen Receptor Beta 2 Antibody | 57/3 gallery image 1

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 2 antibody (MCA2279) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)

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Estrogen Receptor Beta 2 Antibody | 57/3 gallery image 2

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 2 antibody (MCA2279) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)

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Estrogen Receptor Beta 2 Antibody | 57/3 gallery image 3

Published customer image:
Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERß1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERß1, ERß2 and ERß5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.

From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.

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Estrogen Receptor Beta 2 Antibody | 57/3 gallery image 4

Published customer image:
Mouse anti Human estrogen receptor β2 used for the detection of ERβ2 expressing cells in tumor tissue by immunohistochemistry in formalin fixed, paraffin embedded tissue sections.
Image caption:
Effect of gender on ERß1, ERß2 and ERß5 expression in D283 Med xenografts. A) Immunostaining for ERß1 was significantly higher in tumors from females compared to males (**p<0.01); B) and C) Tumor levels of ERß2 and ERß5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score.

From: Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014) Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623.

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Estrogen Receptor Beta 2 Antibody | 57/3 gallery image 5

Published customer image:
Mouse anti Human estrogen receptor β2 used for the detection of ERβ2 expressing cells in normal human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions.

From: Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014) Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623.

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  • Mouse anti Human Estrogen Receptor Beta 2
  • Mouse anti Human Estrogen Receptor Beta 2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    57/3
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2279P *datasheet pdfdatasheet pdf0.1 mg
    MCA2279
    MCA2279GTP *datasheet pdfdatasheet pdf50 µg
    MCA2279GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Estrogen Receptor beta 2 antibody, clone 57/3 recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including the ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform.

      The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide derived from the C terminus of the human estrogen receptor beta 2 isomer, CGMKMETLLPEATMEQ.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)1/501/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Glycine/ EDTA preparations have also been used with success in some tissues
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)1/501/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Glycine/ EDTA preparations have also been used with success in some tissues

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Testis
    • Histology Positive Control Tissue
      Testis
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Estrogen Receptor Beta 2 Antibody Formats

    Formats Clone Applications Sizes available
    Estrogen Receptor Beta 2 Antibody : Purified 57/3 P * 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Testis
              Testis
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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