DAZL Antibody | 3/11A

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DAZL Antibody | 3/11A gallery image 1

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Mouse anti DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in fetal human overy by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Co-localisation of GDF9 with activin βA but not DAZL or BOLL prior to follicle formation. (A) Double immunohistochemistry of 18 week fetal ovary stained for GDF9 (green) and activin βA (red), thus in the merged image co-expression is yellow. Unstained germ cells are indicated with arrows. Counterstain is TOPRO. (B) Triple fluorescent immunohistochemistry for GDF9 (green), DAZL (blue) and BOLL (red) in 20 week human fetal ovary with DAPI as counterstain (grey). Split channel and merged images in (A) and (B) are shown as are merged images of non-immune serum negative control (NEG). Scale bars are 20μm. (C) Nuclear diameters of DAZL, BOLL and GDF9 stained germ cells indicates that GDF9 positive cells are significantly larger (p<0.001) than DAZL but not BOLL expressing cells (bars indicate mean ± sem). (D) Higher magnification merged image of GDF9/DAZL/BOLL immunohistochemistry showing one large primordial follicle is positive for both GDF9 and DAZL but other follicles are positive only for DAZL.

From: Bayne RAL, Kinnell HL, Coutts SM, He J, Childs AJ, et al. (2015) GDF9 is Transiently Expressed in Oocytes before Follicle Formation in the Human Fetal Ovary and is Regulated by a Novel NOBOX Transcript.
PLoS ONE 10(3): e0119819.

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Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL in ovarian and testicular tissue lysates by western blotting.
Image caption:
Western analysis of 1st and 2nd trimester ovaries and testes. In both ovarian (A) and testicular (B) samples VASA (76 kDa) was not detectable in the 1st trimester samples but was present in those from the 2nd trimester. DAZL (33 kDa) was low/undetectable in 1st trimester ovaries (C) whereas it was detectable in ovarian samples from 2nd trimester and testicular samples from both 1st and 2nd trimester (D). OCT4 (42 kDa) was present in both ovaries (E) and testes (F) during both the 1st and 2nd trimester. The loading control in all cases was β-tubulin (51 kDa).

From: Anderson RA, Fulton N, Cowan G, Coutts S, Saunders PT. Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.
BMC Dev Biol. 2007 Dec 18;7:136.

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Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in ovarian tissue by immunofluorescence.
Image caption:
Immunoexpression of OCT4, DAZL and VASA. OCT4 positive germ cell nuclei were detectable in both the 1st and 2nd trimester ovaries (a, 62 d; b, 16 wk) and testes (c, 64 d; d, 16 wk). DAZL positive germ cells were rare in the 1st trimester (e, ovary 61 d; g, testis 64 d) but groups of cells ('nests', labelled N) with cytoplasmic staining were present in the 2nd trimester ovaries (f, 20 wk). During the 2nd trimester VASA protein was detected in the cytoplasm of female germ cells (i, 14 wk; j, 18 wk) throughout the ovary with the exception of the sub-epithelial layer. In the testes (k, 15 wk; l, 16 wk) VASA-positive germ cells were found in all cords.

From: Anderson RA, Fulton N, Cowan G, Coutts S, Saunders PT. Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.
BMC Dev Biol. 2007 Dec 18;7:136.

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Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in ovarian tissue by immunofluorescence.
Image caption:
Co-localisation of OCT4 and DAZL. In 1st trimester ovaries (a, 61 d) and testes (b, 64 d) OCT4 (green) and DAZL (red) were co-localised to germ cell nuclei. In ovaries from the 2nd trimester (c, 14 wk) DAZL protein was almost exclusively cytoplasmic and was largely localised to OCT4 negative groups of cells (arrow); a few OCT4 positive cells had a low level of DAZL immunoexpression in their cytoplasm (arrowheads). Testes, panel b, 64 d; panel d, 16 wk; panel e, 19 wk gestation. In 2nd trimester testes (d, 16 wk; e, 19 wk) DAZL was still expressed in the nuclei of some OCT4 positive germ cells but this pattern of expression was variable with DAZL protein present in the cytoplasm of OCT4 positive and OCT4 negative (arrow panel e) cells.Co-localisation of OCT4 and DAZL. In 1st trimester ovaries (a, 61 d) and testes (b, 64 d) OCT4 (green) and DAZL (red) were co-localised to germ cell nuclei. In ovaries from the 2nd trimester (c, 14 wk) DAZL protein was almost exclusively cytoplasmic and was largely localised to OCT4 negative groups of cells (arrow); a few OCT4 positive cells had a low level of DAZL immunoexpression in their cytoplasm (arrowheads). Testes, panel b, 64 d; panel d, 16 wk; panel e, 19 wk gestation. In 2nd trimester testes (d, 16 wk; e, 19 wk) DAZL was still expressed in the nuclei of some OCT4 positive germ cells but this pattern of expression was variable with DAZL protein present in the cytoplasm of OCT4 positive and OCT4 negative (arrow panel e) cells.

From: Anderson RA, Fulton N, Cowan G, Coutts S, Saunders PT. Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.
BMC Dev Biol. 2007 Dec 18;7:136.

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Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in cynomolgus monkey testes by immunofluorescence on formalin fixed cryosections.
Image caption:
Morphological features of cynomolgus monkey testes, and expression of VASA protein. (A–C) Sections of testes of 3-year-old (A) and 5-year-old monkeys (B,C) were stained with hematoxylin and eosin. Sg, spermatogonium; P, pachytene spermatocyte; Sd, spermatid. (D–Q) Expression of VASA, DAZL, and SCP1 proteins during spermatogenesis in cynomolgus monkeys. Sections of 3-year-old (D,E) and 5-year old testes (F–Q) were examined by immunostaining. The nuclei were stained with Hoechst 33258. Merged images were also shown (E; VASA, red; Hoechst, white), (H, I; VASA, red; DAZL, green; Hoechst, white), (L,M; VASA, red; SCP1, green; Hoechst, white), (P,Q; DAZL, red; SCP1, green; Hoechst, white). VASA expression was observed in spermatogonia in the 3-year-old testis (E, red), and in spermatogonia (open arrowhead), spermatocytes (arrow), and early spermatids (arrowhead) in the 5-year-old testis (I, M, red). The expression of VASA, DAZL, and SCP1 proteins was all detected in spermatocytes (arrow; I, M, Q). The dotted lines indicate the basement membranes of the seminiferous tubules. The scale bar is 25 μm.

From: Yamauchi K, Hasegawa K, Chuma S, Nakatsuji N, Suemori H (2009) In Vitro Germ Cell Differentiation from Cynomolgus Monkey Embryonic Stem Cells.
PLoS ONE 4(4): e5338.

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Mouse anti Human DAZL antibody used for the detection of DAZL expressing cells in rat testis by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry showing localisation of germ cells and 5mC during mid to late gestation. In order to clearly show the localisation of germ cells at all stages of development, we used the specific germ cell cytoplasmic marker DAZL, which survives acid denaturation, in combination with 5mC for immunohistochemistry. The cytoplasm of germ cells is stained for DAZL in blue and 5mC is indicated by brown staining in nuclei. Germ cells (indicated by yellow arrows) are located within seminiferous cords which are surrounded by somatic cells; one seminiferous cord is shown outlined in each image. There are also somatic cells within the seminiferous cords (green arrows). Images show that 5mC is undetectable in germ cells between e14.5 and e18.5 (A to E). 5mC is detectable in germ cells at e19.5 (F, indicated by red arrow) and is more marked in germ cells at e20.5 and e21.5 (G, H). 5mC is present in somatic cells throughout the time course. Scale bar?=?50 μm.

From: Rose CM, van den Driesche S, Sharpe RM, Meehan RR, Drake AJ. Dynamic changes in DNA modification states during late gestation male germ line development in the rat.
Epigenetics Chromatin. 2014 Aug 7;7:19.

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Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in ovarian tissue by immunofluorescence.
Image caption:
Abnormal germ cell proliferation was noted in GR-deficient embryos after sex determination. PGCs were labeled with Dazl (green), and proliferating cells were labeled with Ki67 (red). Most of the germ cells were Ki67-positive at E10.5 (A, B) and E11.5 (E, F), but the Ki67 signal was absent in most germ cells in control embryos after sex determination at E13.5 (I, J, white arrow-heads). In Wt1R394W/R394W embryos, germ cells were labeled with Ki67 at E10.5 (C, D) and E11.5 (G, H), similar to control embryos. However, the germ cells in Wt1R394W/R394W embryos remained Ki67-positive (K, L, white arrows) at E13.5. ce, coelomic epithelium; gr, genital ridge; g, gonad. Scale bars: 30 μm. Histogram summarizing selected percentage of gonocytes that were Ki67+ and note that the percentage of Ki67+ gonocytes were significantly increased in Wt1R394W/R394W embryos at E13.5, compared with control embryos at the same stage (M). The mRNA level of CDKI (p15INK4b, p16Ink4A, P21WAF1/Cip1 and p27Kip1) that inhibited the G1/S transition was estimated by real-time RT-PCR at E12.5 and E13.5 and normalized against PGC-specific marker Stella (N, O). * P <0.05, * * P <0.01. Each bar, mean ± SD of n = 3 experiments.

From: Chen SR, Zheng QS, Zhang Y, Gao F, Liu YX. Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.
BMC Biol. 2013 Mar 5;11:22.

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Published customer image:
Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in ovarian tissue by immunofluorescence.
Image caption:
The PGCs identity was not changed in Wt1R394W/R394W embryos. PGCs identity was examined by double staining of Stella (red) with PGCs characteristic markers, including Oct4 (A), Dazl (B), SSEA-1 (C), and Blimp1 (D). In Wt1R394W/R394W embryos, PGCs still expressed Stella, SSEA-1, Blimp1, Dazl and pluripotent marker Oct4. Scale bars: 50 μm. PGCs-PGCs interactions, such as E-cadherin and β-catenin were maintained in Wt1R394W/R394W embryos (E, F). Scale bars: 50 μm. The mRNA level of PGCs characteristic markers (Oct4, Dazl, SSEA-1, Blimp1, Nanos2, Figla), and PGCs-PGCs interaction markers (E-cadherin and β-catenin) were detected by real-time RT-PCR, and normalized against PGC-specific marker Stella (G). Each bar, mean ± SD of n = 3 experiments.

From: Chen SR, Zheng QS, Zhang Y, Gao F, Liu YX. Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.
BMC Biol. 2013 Mar 5;11:22.

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Mouse anti DAZL antibody, clone 3/11A used for the evaluation of DAZL expression on cultured human spermatogonia by immunofluorescence
Image caption:
Immunohistochemical analysis of (a) hSSC < 2 weeks.Cells were stained with CD49f, DAZL, VASA, VIMENTIN, and STELLA. For VIMENTIN the cultures were negative. Magnification 20x.

From: Sabine Conrad, Hossein Azizi, Maryam Hatami, et al., “Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture,”
BioMed Research International, vol. 2014, Article ID 138350, 17 pages, 2014.

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  • Mouse anti DAZL
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3/11A
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2336IF, P *, WBdatasheet pdfdatasheet pdf2 ml
    MCA2336
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human DAZL antibody, clone 3/11A recognizes human Deleted in azoospermia-like, also known as DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1 or SPGY-like-autosomal. DAZL is a 295 amino acid ~33 kDa member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is predominantly localized to the cytoplasm.

      Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to sequence within the C terminal domain of human DAZL (CRVHHFRRSRAMLKSV).
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised T/O outbred mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      spermatogenesis
      germ cell development
      nucleus
      cytoplasm
      multicellular organismal development
      positive regulation of translational initiation
      RNA binding
      protein binding
      translation activator activity
      nucleotide binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Ovary or testis.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional DAZL Antibody Formats

    Formats Clone Applications Sizes available
    DAZL Antibody : S/N 3/11A IF, P *, WB 2 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Ovary or testis.

              References

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