DAXX Antibody | DAXX-01

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DAXX Antibody | DAXX-01 gallery image 1

Published customer image:
Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in iSLK cells by immunofluorescence and western blotting.
Image caption:
KSHV infection antagonizes the ND10 component ATRX. iSLK cells were seeded onto glass slides, infected with KSHV strain rKSHV.219 for 24A: single staining for PML (c), Sp100 (g) and ATRX (l) B: double staining for ATRX (c) and Daxx (d). Arrows indicate infected cells. C: iSLK cells were infected with KSHV strain rKSHV.219 with high MOI and harvested 20 h post infection. Whole cell lysates were prepared and expression of ND10 components analyzed by immunoblotting.

From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog 10(1): e1003863.

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DAXX Antibody | DAXX-01 gallery image 2

Published customer image:
Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in human foreskin fibroblasts by western blotting.
Image caption:
KSHV ORF75 is sufficient for antagonization of ATRX and Sp100. A: HFF cells were transfected with a pcDNA-orf75-myc expression construct. Cells were analyzed by immunofluorescence with a confocal microscope, and at least 50 individual cells per each box analyzed by Fiji. Statistical analyses were conducted using the unpaired t test for SP100 and PML, and one way ANOVA test for multiple comparisons for Daxx and ATRX, respectively, of GraphPad Prism 6 for Windows. **p<0.01; *** p<0.001. B: 293T cells were transfected with increasing amounts of ORF75 and endogenous expression of ATRX and Daxx was analyzed by immunoblot. C: 293T cells were cotransfected with constant levels of Sp100 and increasing amounts of ORF75 and expression of Sp100 and Daxx levels analyzed by immunoblot.

From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog 10(1): e1003863.

Enlarge
DAXX Antibody | DAXX-01 gallery image 3

Published customer image:
Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in human foreskin fibroblasts by immunofluorescence.
Image caption:
Diffuse localization of ATRX after knockdown of Daxx. HFF cells carrying retroviral knockdown shRNA vectors targeting Daxx or PML were immunostained with respective antibodies. ND10 accumulation as shown by colocalization (c) with PML (a) of ATRX (b) is lost in Daxx-kd cells (e,f) while PML (d) remains in ND-10 structures. In contrast, knockdown of PML (g–i) results in dispersal of ND10 and seemingly a partial colocalization (i) of Daxx (g) and ATRX (h) into smaller structures; most of Daxx and ATRX proteins are not colocalized.

From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog 10(1): e1003863.

Enlarge
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  • Mouse anti Human DAXX
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    DAXX-01
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2143C, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2143
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human DAXX antibody, clone DAXX-01 recognizes human Death domain-associated protein 6, also known as DAXX, ETS1-associated protein 1, EAP1 or Fas death domain-associated protein. DAXX is a 740 amino acid ~81 kDa nucleoplasmic protein.

      DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional DAXX Antibody Formats

    Formats Clone Applications Sizes available
    DAXX Antibody : Purified DAXX-01 C, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
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              References

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