Complement Factor H Antibody | OX-23

Complement Factor H Antibody | OX-23 gallery image 1

Published customer image:
Mouse anti Human factor H antibody, clone OX-23 (MCA508G) used as a capture reagent in a sandwich ELISA for HfH levels in murine sera with Sheep anti Human complement factor H antibody (4400-9504) as a detection reagent
Image caption:
Effect of the presence of hfH in mice on the nOMV immunogenicity.
A. Evolution of hfH concentration in mouse serum over time after two IP injection of 0.5mg purified hfH (black arrows) 24 and 0.5 hours prior to immunization (grey arrow). B. Serum Bactericidal Assay (SBA) titers against 44/76-SL wild-type in pooled sera from C57bl/6 mice pre-injected with 800 µg/ml hfH or hSA, 14 days following immunization with 5 &mu:g of nOMVlpxl1 or nOMVdis. C. Individual SBA titers against 44/76-SL, dots represent individual animals D. Individual ELISA titers against rfHbp induced after one dose of H44/76Lpxl1 OMV in mice pre-injected with hfH or hSA, *p = 0.016, not all sera were available. C, D dots represent individual animals, the geometric mean and 95% confidence interval are represented as horizontal bars.

From: Daniels-Treffandier H, de Nie K, Marsay L, Dold C, Sadarangani M, Reyes-Sandoval A, et al. (2016)
Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles.
PLoS ONE 11(2): e0148840.

  • Complement Factor H Antibody | OX-23 thumbnail image 1
  • Mouse anti Human Complement Factor H
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA508GE, IP, R, WBdatasheet pdfdatasheet pdf0.2 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human Complement Factor H antibody, clone OX-23 recognizes intact human serum complement protein factor H, also known as H factor 1. Complement factor H is a 1213 amino acid ~155 kDa secreted glycoprotein bearing multiple disulphide bonds and is involved in the deactivation of C3b and dissociation of C3 convertase in the alternative complement pathway. Mouse anti Human Complement Factor H antibody, clone OX-23 also recognizes a ~43-49 kDa truncated form of factor H present at low level (1-5ug/ml) in plasma and urine.

      Mutations in the CFH gene can lead to the development of Complement Factor H deficiency (CFHD) which can be asymptomatic, present with recurrent bacterial infections or renal failure (Ault et al. 1997). Mutations can also lead to development of Basal laminar drusen (BLD), the deposition of extracellular deposits accumulating below the retinal pigment epithelium on Bruch membrane which can ultimately lead to visdion loss (Boon et al. 2008). Additionally, mutations in the CFH gene can lead to increased susceptibility to Hemolytic uremic syndrome atypical 1 (AHUS1) or Macular degeneration, age-related, 4 (ARMD4).

      Mouse anti Human Complement Factor H antibody, clone OX-23 has been used successfully for the determination of levels of bound murine factor H in a sandwich ELISA in conjunction with Sheep anti Human Factor H antibody (4400-9504 ) as a capture reagent (Daniels-Treffandier et al. 2016).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Human complement factor H.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the NS-O mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Complement Factor H Antibody Formats

    Formats Clone Applications Sizes available
    Complement Factor H Antibody : Purified OX-23 E, IP, R, WB 0.2 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting


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