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CD96 antibody | NK92.39

Product Code Applications Pack Size List Price Your Price Qty
MCA4643
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
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loader

Mouse anti Human CD96 antibody, clone NK92.39 recognizes human CD96, otherwise known as Tactile (T cell-activated increased late expression), a type I transmembrane glycoprotein expressed at low levels on peripheral resting T and NK cells, which increases following activation.

CD96 stimulates the cytotoxicity of activated NK cells, and promotes the adhesion of NK cells to target cells expressing CD155/poliovirus receptor (PVR) and the transfer of target cell PVR to the NK cells. CD96 can be used in the immunophenotyping of T-cell acute lymphoblastic leukaemias (ALL) and acute myeloid leukaemias (AML), and may play a role in the recognition of CD155-expressing tumours by NK cells.

Mouse anti Human CD96 antibody, clone NK92.39 blocks the binding of soluble CD155 to NK92 cells (Fuchs et al. 2004).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Natural killer 92 (NK92) cell line.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD96 antibody

  1. Fuchs, A. et al. (2004) Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).
    J Immunol. 172 (7): 3994-8.

Synonyms
Tactile
RRID
AB_1628787
UniProt
P40200
Entrez Gene
CD96
GO Terms
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0050776 regulation of immune response

MCA4643

150983 158802

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