CD71 Antibody | DF1513

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CD71 Antibody | DF1513 gallery image 1

Staining of MOLT4 ( human acute lymphoblastic leukemia) cells with Mouse anti Human CD71: FITC (MCA1148F)

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CD71 Antibody | DF1513 gallery image 2

Staining of Jurkat cells with Mouse anti Human CD71:RPE (MCA1148PE)

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CD71 Antibody | DF1513 gallery image 3

Staining of KG1 cells with Mouse anti Human CD71:Alexa Fluor® 647 (MCA1148A647)

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  • CD71 Antibody | DF1513 thumbnail image 1
  • CD71 Antibody | DF1513 thumbnail image 2
  • CD71 Antibody | DF1513 thumbnail image 3
  • Mouse anti Human CD71:FITC
  • Mouse anti Human CD71:RPE
  • Mouse anti Human CD71:FITC
  • Mouse anti Human CD71:RPE
  • Mouse anti Human CD71
  • Mouse anti Human CD71
  • Mouse anti Human CD71
  • Mouse anti Human CD71:Low Endotoxin
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  • Product Type
    Monoclonal Antibody
  • Clone
    DF1513
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1148FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1148F
    MCA1148GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA1148GA
    MCA1148F, IFdatasheet pdfdatasheet pdf0.2 mg
    MCA1148
    MCA1148ELF, IFdatasheet pdfdatasheet pdf0.5 mg
    MCA1148EL
    MCA1148PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1148PE
    MCA1148FTFdatasheet pdfdatasheet pdf25 µg
    MCA1148FT
    MCA1148TF, IFdatasheet pdfdatasheet pdf25 µg
    MCA1148T
    MCA1148PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1148PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a 190kDa homodimeric glycoprotein expressed by proliferating cells. CD71 is also known as the transferrin receptor.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rhesus Monkeyyes
      Mustelidyes
      Ferretyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      None present
    • Immunogen
      KG1 cell line
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
      endocytosis
      transmembrane transport
      cellular iron ion homeostasis
      endosome
      melanosome
      integral to plasma membrane
      transferrin receptor activity
      interspecies interaction between organisms
      proteolysis
      peptidase activity
      transferrin transport
      coated pit
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD71 Antibody Formats

    Formats Clone Applications Sizes available
    CD71 Antibody : Purified DF1513 F, IF 25 µg | 0.1 mg | 0.2 mg
    CD71 Antibody : RPE DF1513 F 100 Tests | 25 Tests
    CD71 Antibody : FITC DF1513 F 25 µg | 0.1 mg
    CD71 Antibody : Low Endotoxin DF1513 F, IF 0.5 mg
    • Copyright © 2016 Bio-Rad

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              • Application NameReference Images
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              References

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