CD56 Antibody | MEM-188

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CD56 Antibody | MEM-188 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD56 (MCA2046)

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CD56 Antibody | MEM-188 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD56:Alexa Fluor® 647 (MCA2046A647)

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CD56 Antibody | MEM-188 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD56:Biotin (MCA2046B)

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CD56 Antibody | MEM-188 gallery image 4

Published customer image:
FITC conjugated Mouse anti Human CD56 antibody, clone MEM-188 used for the identificationof CD56 positive cells from human peripheral blood mononuclear cell populations by flow cytometry.
Image caption:
Intracellular GM-CSF staining on CD56+ cells from hPBMC. (A) In the presence of the indicated ODN plus IL2: GM-CSF content in the cell culture without ODN stimulation was used as a control. Data are shown as mean +SD and are representative of three similar experiments. A specific response was defined as a response where the percentage of GM-CSF in CD56+ lymphocytes was at least 2-fold below the background observed in the medium control. The asterisk indicates statistically significant differences (p<0.05). (B) In the presence of IL2 in both CD3+ (NKT) and CD3- (NK) CD56+ dim or bright populations: ODNs and/or IL2 were added to the medium to reach a final concentration of 6 µg/ml and 400 IU/ml respectively. Dot plots are representative of three similar experiments.

From: Rodriguez JM, Marchicio J, López M, Ziblat A, Elias F, et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells. PLoS ONE 10(2): e0117484.

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CD56 Antibody | MEM-188 gallery image 5

Published customer image:
AlexaFluor-647 conjugated Mouse anti Human CD56 antibody used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.
Image caption:
HLA-DR+ Accessory Cells Provide Activating Signals to NK Cells PBMCs from malaria-naïve donors were depleted of different populations of accessory cells, and NK cell responses to iRBCs were analysed after 24 h of co-culture. (A) Representative example of NK cell IFN-? responses in undepleted PBMCs (top left), PBMCs depleted of all HLA-DR+ antigen-presenting cells (APC) (top right), CD14+ monocyte–depleted PBMCs (middle left), CD19+ B cell–depleted PBMCs (middle right), CD1c+ mDC–depleted PBMCs (bottom left), and BDCA-4+ pDC–depleted PBMCs (bottom right). FACS plots are gated on CD3- CD56+ lymphocytes. Percentages indicate the proportion of CD3- CD56+ NK cells that were positive for IFN-?; data are based on the collection of 100,000 total events. (B) NK cell IFN-? production after depletion of different antigen-presenting cell populations. Percentage of IFN-? NK cells relative to the response in undepleted PBMCs is shown; each spot represents data for a different donor. *, p < 0.05; ***, p < 0.0001; p–values are for paired t tests of the difference between depleted and undepleted cells:

From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006) Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-? Responses to Malaria. PLoS Pathog 2(12): e118.

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CD56 Antibody | MEM-188 gallery image 6

Published customer image:
AlexaFluor-647 conjugated Mouse anti Human CD56 antibody used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.
Image caption:
NK Cells Do Not Respond to iRBCs in the Absence of Accessory Cells PBMCs or MACS-purified NK cells from malaria-naïve donors were cultured alone or with IL-12 and IL-18, uRBCs, or live iRBCs for 24 h. Intracellular IFN-? and surface expression of CD69 on CD3- CD56+ NK cells were analysed by flow cytometry. (A and B) Representative example of NK responses to uRBCs, IL-12 and IL-18, and iRBCs in PBMCs (A) and purified NK cells (B). FACS plots are gated on CD3- CD56+ lymphocytes. Percentages indicate the proportion of CD3- CD56+ NK cells in each gate; data are based on the collection of 100,000 total events. (C and D) Comparison of NK responses to iRBCs in PBMCs and in purified NK cells. (C) NK cell IFN-? production for six donors. (D) CD69 surface expression levels for seven donors. **, p < 0.01; ***, p < 0.001; p-values are for paired t tests comparing stimulated and unstimulated NK cells from the same donor, or PBMCs and pure NK cells from the same donor. (E) MACS-purified, CTG-labelled NK cells were added to autologous PBMCs. IFN-? production in CTG+ (MACS-purified) and CTG- (“untouched”) NK cells was assessed by flow cytometry after 24 h of stimulation with iRBCs. A representative example of the software gating strategy to analyse IFN-? responses of CTG-labelled NK cells is shown. FACS plots were gated on CD3- CD56+ NK cells within the lymphocyte population (first panel), and NK cells were analysed for CTG staining (second panel). CTG+ CD3- CD56+ NK cells (third panel) or CTG- CD3- CD56+ NK cells (fourth panel) were selected and analysed for intracellular IFN-? production. Numbers indicate the proportion of IFN-?+ NK cells.

From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006) Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-? Responses to Malaria. PLoS Pathog 2(12): e118.

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  • Mouse anti Human CD56
  • Mouse anti Human CD56
  • Mouse anti Human CD56
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-188
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2046GAC *, F, IP, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA2046GA
    MCA2046C *, F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA2046
    MCA2046TC *, F, IP, WB*datasheet pdfdatasheet pdf25 µg
    MCA2046T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD56 antibody, clone MEM-188 recognizes CD56 a 140kDa cell surface glycoprotein expressed primarily by natural killer cells and a subset of T cells in the peripheral blood.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.2 mg, 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mg, 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mg, 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/25
      1/100Pack Size: 0.2 mg, 25 µg
      1/50Pack Size: 0.1 mg
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/40
      Western Blotting(2)Non-reducing conditions
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      MEM-188 recognizes CD56 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/25
      1/100Pack Size: 0.2 mg, 25 µg
      1/50Pack Size: 0.1 mg
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/40
      Western Blotting(2)Non-reducing conditions
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      MEM-188 recognizes CD56 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/25
      1/100Pack Size: 0.2 mg, 25 µg
      1/50Pack Size: 0.1 mg
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/40
      Western Blotting(2)Non-reducing conditions
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      MEM-188 recognizes CD56 under non-reducing conditions.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD56 Antibody Formats

    Formats Clone Applications Sizes available
    CD56 Antibody : Purified MEM-188 C *, F, IP, WB* 0.1 mg | 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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              References

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