CD55 Antibody | 67

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CD55 Antibody | 67 gallery image 1

Figure A. A700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse anti Human CD55 (MCA1614PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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CD55 Antibody | 67 gallery image 2

Published customer image:
Mouse anti Human CD55 antibody, clone 67 (MCA1614) used for the identification of CD55 expressing cells in rheumatoid arthritis synovial tissue by immunohistochemistry on cryostat sections.
Image caption:
Representative double staining of rheumatoid arthritis synovial tissue with antibodies against RasGRF1 and cell-specific markers. Synovial tissue sections were stained overnight with antibodies against Ras guanine nucleotide-releasing factor 1(RasGRF1), followed by antibodies against CD3, CD55, and CD68. After biotin tyramide enhancement, staining was developed with amino-ethylcarbazole (red, RasGRF1) and Fast blue (blue, cell-specific markers). Magnification x 100.

From: Abreu JR, de Launay D, Sanders ME, Grabiec AM, van de Sande MG, Tak PP, Reedquist KA.
The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue.
Arthritis Res Ther. 2009;11(4):R121.

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CD55 Antibody | 67 gallery image 3

Published customer image:
Mouse anti CD55 antibofdy, clone 67 (MCA1614) used for the demonstration of CD55 and MPGES1 in fibroblasts from sinovial lining by immunofluorescence.
Image caption:
MPGES1 expression in synovial lining fibroblasts before and 16 weeks after initiation of rituximab therapy.
Double immunofluorescence pictures show the presence of MPGES1 (red) expression in CD55 positive fibroblasts (green) in the rheumatoid tissue before rituximab initiation (A) and 16 weeks later (B). Original magnification 500x. Arrows point to double stained cells

From: Gheorghe KR, Thurlings RM, Westman M, Boumans MJ, Malmström V, Trollmo C, et al. (2011)
Prostaglandin E2 Synthesizing Enzymes in Rheumatoid Arthritis B Cells and the Effects of B Cell Depleting Therapy on Enzyme Expression.
PLoS ONE 6(1): e16378.

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  • CD55 Antibody | 67 thumbnail image 1
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  • Mouse anti Human CD55
  • Mouse anti Human CD55:RPE
  • Mouse anti Human CD55
  • Mouse anti Human CD55:RPE
  • Mouse anti Human CD55
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    67
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1614GAC, F, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA1614GA
    MCA1614C, F, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1614
    MCA1614PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1614PE
    MCA1614TC, F, WBdatasheet pdfdatasheet pdf25 µg
    MCA1614T
    MCA1614PETFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1614PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD55 antibody, clone 67 recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak.

      CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens.

      DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta (Holmes et al. 1990 and Yang et al. 2009). Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled water
    • Preparation
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 100 TestsPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 25 Tests/0.25mlPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 100 TestsPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 25 Tests/0.25mlPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
    • Immunogen
      K562 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      elevation of cytosolic calcium ion concentration
      soluble fraction
      anchored to membrane
      respiratory burst
      innate immune response
      integral to plasma membrane
      complement activation, classical pathway
      extracellular region
      membrane raft
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunohistology - Frozen1/1001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunohistology - Frozen1/1001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunohistology - Frozen1/1001/1000
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD55 Antibody Formats

    Formats Clone Applications Sizes available
    CD55 Antibody : Purified 67 C, F, WB 25 µg | 0.1 mg | 0.2 mg
    CD55 Antibody : RPE 67 F 25 Tests/0.25ml | 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              Human Tonsil
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              • Application NameReference Images
                Immunohistology - Frozen
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              References

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