CD257 Antibody | 2B

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  • Mouse anti Human CD257
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2B
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2677Edatasheet pdfdatasheet pdf0.5 mg
    MCA2677
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD257 antibody, clone 2B recognises CD257, also known as BAFF/BLyS. CD257 is a 285 amino acid type II transmembrane protein and member of the tumor necrosis factor (TNF) superfamily of ligands, which acts as a B and T cell stimulator and plays a role in the development of autoimmune diseases such as systemic lupus erythematosus.

      CD257 is expressed by monocytes, macrophages, T cells and dendritic cells and acts through binding to CD267 (TACI), CD269 (BCMA) and CD268 (BAFFR) receptors, the latter interaction playing a critical role in the activation, survival, differentiation and proliferation of B cells. Mouse anti Human CD257 antibody, clone 2B has not been cross-adsorbed and may react with homologous proteins from related species.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant 17 kDa polypeptide corresponding to the TNF-like portion of the extracellular domain of human CD257, expressed in E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      soluble fraction
      integral to membrane
      cytokine activity
      immune response
      extracellular space
      tumor necrosis factor receptor binding
      cell proliferation
      plasma membrane
      signal transduction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA56ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD257 Antibody Formats

    Formats Clone Applications Sizes available
    CD257 Antibody : Purified 2B E 0.5 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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