CD25 Antibody | MEM-181

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CD25 Antibody | MEM-181 gallery image 1

Staining of PHA activated human peripheral blood lymphocytes with Mouse anti Human CD25 (MCA2127)

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CD25 Antibody | MEM-181 gallery image 2

Published customer image:Potency of Treg-suppression is dependent on strength of stimulus and on Effector:Treg cell (E:T) ratio. (A) Typical FACS plot displaying purity of effector (CD4+CD25-) and Treg (CD4+CD25+) populations in healthy control subjects. (B) Mean counts per minute (CPM) (+SEM) uptake of tritiated thymidine in CD25- effector T cells from healthy controls (N = 27) stimulated with mitogenic CD3/28 beads at 2:1 and 0.2:1 bead: cell ratios. (C) Mean CPM (+SEM) in CD25- effectors v CD25+ Treg cells stimulated with mitogenic CD3/28 beads at 2:1 bead: cell ratio. (D) Mean (+SEM) percentage suppression of effector cell proliferation following activation with CD3/28 beads at 2:1 (closed boxes) and 0.2:1 (open boxes) bead: cell ratios.

From: Thorborn G, Pomeroy L, Isohanni H, Perry M, Peters B, et al. (2010) Increased Sensitivity of CD4+ T-Effector Cells to CD4+CD25+ Treg Suppression Compensates for Reduced Treg Number in Asymptomatic HIV-1 Infection. PLoS ONE 5(2): e9254.

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CD25 Antibody | MEM-181 gallery image 3

Published customer image:
Detection of circulating CD4+CD25low/-GITR+cells in SLE patients.(A) Flow-cytometry analysis of anti-GITR and anti-CD25-stained CD4+ T lymphocytes, freshly isolated from blood of a representative SLE patient, reveals the presence of a CD25-GITR+ cell subpopulation. Left panel: gating strategy. Gated CD4+ T lymphocytes were stained with isotype control Abs (middle panel) or anti-GITR and anti-CD25 Abs (right panel). (B) the levels of CD25 expression were evaluated in the upper left and lower left quadrants and MFI is reported. (C) mRNA expression of CD25 (FAM6 fluorochrome) in the indicated subpopulations was evaluated in quadruplicate with real-time PCR. In the same tube, expression of the housekeeping gene HPRT1 (VIC fluorochrome) was evaluated for normalization. Values of CD25 expression (striped column, CD25highGITR-; black column, CD25low/-GITR+) is shown as fold increase of mRNA levels in the positively sorted subpopulations over mRNA levels in effector (CD4+CD25-GITR-) T cells, arbitrarily set equal to 1. Data shown are mean?±?SD of four SLE patients. ***P <0.001.

From: Nocentini et al. Expansion of regulatory GITR+CD25low/-CD4+ T cells in systemic lupus erythematosus patients Arthritis Research & Therapy 2014, 16:444

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CD25 Antibody | MEM-181 gallery image 4

Figure A. Alexa®647 conjugated mouse anti human CD4 (MCA1267A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa®647 conjugated mouse anti human CD4 (MCA1267A647) and RPE conjugated mouse anti human CD25 (MCA2127PE). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A)

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  • CD25 Antibody | MEM-181 thumbnail image 1
  • CD25 Antibody | MEM-181 thumbnail image 2
  • CD25 Antibody | MEM-181 thumbnail image 3
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  • Mouse anti Human CD25:Alexa Fluor® 647
  • Mouse anti Human CD25:RPE
  • Mouse anti Human CD25
  • Mouse anti Human CD25:FITC
  • Mouse anti Human CD25:Biotin
  • Mouse anti Human CD25:Alexa Fluor® 647
  • Mouse anti Human CD25:FITC
  • Mouse anti Human CD25
  • Mouse anti Human CD25:Alexa Fluor® 488
  • Mouse anti Human CD25:Biotin
  • Mouse anti Human CD25:RPE
  • Mouse anti Human CD25:Alexa Fluor® 488
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-181
  • Isotype
    IgG1
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2127FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2127F
    MCA2127BFdatasheet pdfdatasheet pdf0.1 mg
    MCA2127B
    MCA2127Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2127
    MCA2127PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2127PE
    MCA2127A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2127A647
    MCA2127A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2127A488
    MCA2127FTFdatasheet pdfdatasheet pdf25 µg
    MCA2127FT
    MCA2127TFdatasheet pdfdatasheet pdf25 µg
    MCA2127T
    MCA2127BTFdatasheet pdfdatasheet pdf25 µg
    MCA2127BT
    MCA2127PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2127PET
    MCA2127A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2127A647T
    MCA2127A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2127A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD25, clone MEM-181 recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein with two Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Norman et al. 1991) located within its extracellular domain.

      The IL-2 receptor exists in three forms. A high affinity form consiting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer.

      CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attia et al. 2010).

      Elevated levels of CD25 antigen expression are often seen in cases of non-Hodgkin 's lymphoma and diffuse large B cell lymphoma (Fujiwara et al.2013).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Human PHA blasts; day 3 of culture.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      interleukin-2 receptor activity
      cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : Purified MEM-181 F 25 µg | 0.2 mg
    CD25 Antibody : Alexa Fluor® 647 MEM-181 F 25 Tests/0.25ml | 100 Tests/1ml
    CD25 Antibody : Biotin MEM-181 F 25 µg | 0.1 mg
    CD25 Antibody : FITC MEM-181 F 0.1 mg | 25 µg
    CD25 Antibody : Alexa Fluor® 488 MEM-181 F 25 Tests/0.25ml | 100 Tests/1ml
    CD25 Antibody : RPE MEM-181 F 25 Tests | 100 Tests
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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