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CD236 Antibody | BRIC10
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Enlarge- Mouse anti Human CD236
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Product TypeMonoclonal Antibody
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CloneBRIC10
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human CD236 antibody, clone BRIC10 recognizes an epitope in the N-terminal region of CD236, also known as glycophorin C (GPC). CD236 is an integral membrane protein which carries the Gerbich blood group antigens. Expression of CD236 is not restricted to erythroid cells, but is broadly distributed in both erythroid and non-erythroid tissues.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein G.
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Preservative Stabilisers
0.09% Sodium Azide -
ImmunogenHuman erythrocytes.
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionTRIS buffered glycine
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StorageStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO Termscortical cytoskeletonintegral to plasma membraneprotein binding
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry Western Blotting - Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 106 cells in 100ul.
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional CD236 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD236 Antibody : Purified | BRIC10 | F, WB | 0.2 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | MCA928 |
Useful Reagents
Recommended Positive Controls
Histology Controls
References
1. Dahr, W. et al. (1989) High-frequency antigens of human erythrocyte membrane sialoglycoproteins. VI. Monoclonal antibodies reacting with the N-terminal domain of glycophorin C.Biol Chem Hoppe Seyler. 370 (8): 849-54.
2. Anstee, D.J. et al. (1984) Individuals lacking the Gerbich blood-group antigen have alterations in the human erythrocyte membrane sialoglycoproteins beta and gamma.Biochem J. 221 (1): 97-104.
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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