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CD22 antibody | MEM-01

Mouse anti Human CD22

Product Type
Monoclonal Antibody
Clone
MEM-01
Isotype
IgG1
Specificity
CD22

Product Code Applications Pack Size List Price Your Price Qty
MCA2331
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB * 0.2 mg loader
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loader

Mouse anti Human CD22 antibody, clone MEM-01 recognizes the human CD22 cell surface antigen, a ~130/140 kDa glycoprotein expressed by cells of B lymphocyte lineage.

CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. Expression is lost upon terminal differentiation to plasma cells.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Burkitt's lymphoma cell line Raji
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunoprecipitation
Western Blotting 1
  1. 1Clone MEM-01 recognizes human CD22 under non-reducing conditions. PTR
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Western Blotting
Clone MEM-01 detects a band of approximately 140kD in cell lysates under non reducing conditions.

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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Synonyms
BL-CAM
RRID
AB_566644
UniProt
P20273
Entrez Gene
CD22

MCA2331

155990 1612

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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