CD146 Antibody | OJ79c

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CD146 Antibody | OJ79c gallery image 1

Published customer image:
Mouse anti Human CD146 antibody, clone OJ79c (MCA2141) used for the evaluation of CD146 expression on brain derived cell lines by flow cytometry.
Image caption:
Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-β, RGS5, CD146, Nestin, α-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).

From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.
PLoS ONE 7(4): e35577.

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CD146 Antibody | OJ79c gallery image 2

Published customer image;
Mouse anti Human CD146 antibody, clone OJ79c (MCA2141) used for the evaluation of CD146 expression on cell lines and compared to binding of porphyrin conjugated antibodies by flow cytometry.
Image caption
Conjugated and unconjugated anti-CD326, anti-CD146 and anti-CD104 were analysed by flow cytometry for binding to CORL23 and LoVo cells [black line, negative control; blue line, unconjugated antibody; red line, immunoconjugates with porphyrin 1 (a) or porphyrin 2 (b)].

From: Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, Greenman J, Boyle RW. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Immunology. 2011 Feb;132(2):256-65.

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CD146 Antibody | OJ79c gallery image 3

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Mouse anti HumanCD146 antibody, clone OJ79c (MCA2141) used for the identification of endothelial cell derived non-annexin V binding, circulating microparticles by flow cytometry.
Image caption:
Plasma levels of annexin V non-binding, cell-derived microparticles (AnxV- MPs) correlated to lung function parameters. Endothelial cell derived MPs and leukocyte derived MPs (log counts/mL) correlated with both carbon monoxide diffusing capacity in percent of predicted values (DLco, %) (r?=?-0.28; p?=?0.003, and r?=?-0.26; p?=?0.005) respectively (a and b) and forced vital capacity in percent of predicted values (FVC, %) (r?=?-0.24; p?=?0.009, and r?=?-0.29; p?=?0.002) respectively (c and d). No correlations between AnxV- platelet derived MPs and lung function parameters were found (e and f). Correlation analysis was performed by use of Pearson's r test using log transformed microparticle data.

From: Iversen LV, Ullman S, Østergaard O, Nielsen CT, Halberg P, Karlsmark T, Heegaard NH, Jacobsen S. Cross-sectional study of soluble selectins, fractions of circulating microparticles and their relationship to lung and skin involvement in systemic sclerosis. BMC Musculoskelet Disord. 2015 Aug 12;16:191.

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CD146 Antibody | OJ79c gallery image 4

Published customer image:
AlexaFluor 647 conjugated Mouse anti Human CD146 antibody, clone OF79c (MCA2141A647) used for the detection of CD146 expressing cells by immunofluorescence.
Image caption:
(A) Pericytes expressing CD146 reside on the abluminal surface of endothelial cells expressing vWF. (B) αSMA expressing pericytes co-express MSC markers including CD90. (C) In-vitro pericytes express MSC markers including CD105. Cultured pericytes are multipotent as seen by their ability to differentiate into bone (D) (Alizarin red staining ×10), fat (E) (Oil Red O staining × 10), cartilage (F) (Alcian blue staining × 5).

From: Tawonsawatruk, T. et al. Adipose derived pericytes rescue fractures from a failure of healing – non-union. Sci. Rep. 6, 22779.

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CD146 Antibody | OJ79c gallery image 5

Published customer image:
AlexaFluor 647 conjugated Mouse anti Human CD146 antibody, clone OF79c (MCA2141A647) used for the detection of CD146 expressing cells by flow cytometry.
Image caption:
(A) Pericytes can be purified from the SVF of adipose tissue by FACS. Cells (circled in red) are selected on an initial FSC v SSC dot plot. (B) Following removal of dead cells, haematopoietic (CD45+) and endothelial cells (CD31+), pericytes (red box) can be selected based on their unique phenotype (CD146+, CD34-, CD31-, CD45-). (C,D) Pericytes maintain a stable phenotype over extended periods of culture (CD146+, CD31-).

From: Tawonsawatruk, T. et al. Adipose derived pericytes rescue fractures from a failure of healing – non-union. Sci. Rep. 6, 22779.

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CD146 Antibody | OJ79c gallery image 6

Published customer image:
Mouse anti Human CD146 antibody, clone oJ79c (MCA2141) used to identify CD146 expressing pericytes in human fetal and embryonic kidneys by immunofluorescence
Image caption:
Identification of pericytes in human embryonic and fetal kidneys. (a) Fetal pericytes were labeled with antibodies against CD146 (green) and NG2 (red) in a 15-week-old kidney cortex. CD146+NG2+ pericytes were found in peritubular capillaries (arrow), mesangial cells (open arrowheads), and afferent arterioles (arrowheads). (b) In the kidney medulla, pericytes encircled peritubular capillaries (arrows) and the vasa recta (arrowheads). (c) Double staining for CD146 (red) and smooth muscle α-actin (αSMA) (green) shows that in the cortex, pericytes coexpress αSMA in the mesangium (open arrowheads) and afferent arterioles (arrowheads). In embryonic pericytes, sections of 7-week-old kidneys were stained for pericyte markers: CD146 (green), nerve/glial antigen 2 (NG2) (green), and αSMA (green), with the endothelial cell marker CD31 (red) (on consecutive sections). (d) αSMA immunoreactivity was sparse; only single vascular smooth muscle cells were found (inset, arrow). (e) NG2+ cells surrounded endothelial cells (inset, arrow). (f) CD146 staining was perivascular (inset, arrow) and in endothelial cells (inset, arrowhead). DAPI, 4',6-diamidino-2-phenylindole.

From: Stefanska A, Kenyon C, Christian HC, Buckley C, Shaw I, Mullins JJ, Péault B.
Human kidney pericytes produce renin.
Kidney Int. 2016 Dec;90(6):1251-61.

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CD146 Antibody | OJ79c gallery image 7

Published customer image:
Mouse anti Human CD146 antibody, clone oJ79c (MCA2141) used to identify CD146 expressing pericytes in human fetal kidneys by immunofluorescence.
Image caption:
Renin is colocalized with pericyte markers in the human fetal kidney. Triple staining of fetal kidney demonstrates that (a) renin immunoreactivity (red) coincides with pericyte marker CD146 (green) expression in afferent arterioles. CD146 staining is also present in the mesangium (open arrowhead). Characteristically, renin staining had a striped pattern. The inset (a') shows an afferent arteriole at high magnification. Renin and CD146 stainings overlap on the abluminal side of the vessel (arrowheads), whereas immunoreactivity for CD144 (grey), an endothelial cell marker, is visible inside blood vessels (arrow). (b) Nerve/glial antigen 2 (NG2) (green) staining is found in the mesangium and afferent arteriole. Renin (red) is present in the JG area. Inset (b') shows renin+ cells coexpressing NG2 (arrowheads) at higher magnification. JG, juxtaglomerular cell.

From: Stefanska A, Kenyon C, Christian HC, Buckley C, Shaw I, Mullins JJ, Péault B.
Human kidney pericytes produce renin.
Kidney Int. 2016 Dec;90(6):1251-61.

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CD146 Antibody | OJ79c gallery image 8

Published customer image:
Mouse anti Human CD146 antibody, clone oJ79c (MCA2141) used to identify CD146 expressing pericytes in human fetal kidneys by flow cytometry and to demonstrate CD146 positivity in pericytes cultured in vitro by immunofluorescence.
Image caption:
Isolation and in vitro characterization of pericytes from a human fetal kidney. (a) FACS of renal pericytes purified from a 16-week human fetal kidney. Dot plots show backgating strategy to obtain CD146+CD34-CD56-CD45- pericytes. Red-color dots show gated populations and the percentages of the parent gates. The sorted pericyte population contained no CD45+, CD56+, or CD34+ cells. (b) Cytospins of freshly sorted pericytes were immunostained for renin (red, arrowheads). (c) Granular, intracellular expression of renin (red) can be observed for up to 48 hours in occasional cells of renal pericyte primary cultures. Positively stained cells are native JG cells, which retain renin expression for a short term in vitro. (d) Representative results of gene expression profiles of renal pericyte primary cultures (n ≥ 3). Pericytes showing expression of the pericyte markers CD146, nerve/glial antigen 2 (NG2), and platelet-derived growth factor receptor-β (PDGFRβ), were negative for hematopoietic CD45 and metanephric mesenchyme CD56 expressions. In addition, pericyte cultures were enriched in Foxd1 (stromal marker) and CRIM1 (pericyte/parietal cell/podocyte marker). (e) Morphology of cultured CD146+CD34-CD56-CD45- pericytes. Bright field images of renal pericyte primary cultures are shown after plating at passages (p) 0, 2, and 6. Cells displayed a spindle-shaped morphology at p0 and p2 but eventually acquired fibroblastic-like appearance at p6. (f) Cultured kidney pericytes at p2 show some α-smooth muscle actin (αSMA) (green) positive staining, whereas most of the cells are positive for NG2 (green) and all cultured pericytes are CD146+ (green). (g) Kidney pericyte primary cultures underwent mesodermal lineage induction. After 14 days of differentiation, primary cultures were confirmed to differentiate along adipogenic (Oil red O staining), chondrogenic (Alcian blue staining), and osteogenic lineages (Alizarin red staining). Control cells were incubated with growth medium (not shown). Original magnifications are shown on the images. DAPI, 4',6-diamidino-2-phenylindole; FACS, fluorescence-activated cell sorter; FSC, forward scatter; JG, juxtaglomerular cell; K, kidney (positive control); NTC, no template control; SSC, side scatter.

From: Stefanska A, Kenyon C, Christian HC, Buckley C, Shaw I, Mullins JJ, Péault B.
Human kidney pericytes produce renin.
Kidney Int. 2016 Dec;90(6):1251-61.

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CD146 Antibody | OJ79c gallery image 9

Published customer image:
Mouse anti Human CD146 antibody, clone oJ79c (MCA2141) used to identify CD146 expressing pericytes in human fetal kidneys by immunofluorescence.
Image caption:
CD146+NG2+ αSMA+/- renal pericytes express and secrete enzymatically active renin in vitro. Renal pericyte primary cultures at passage 2 were stained for renin (red). (a) Control cells (vehicle: medium + vehicle; untreated cells: medium) show no renin immunoreactivity. Forskolin and isobutyl-1-methylxanthine (cyclic adenosine monophosphate induction) treatment in pericytes (b) caused robust renin staining, (c) increased renin mRNA expression, and (d) renin activity. One-way analysis of variance followed by Bonferroni's post hoc comparisons were used to test statistical significance. Data are shown as mean ± SEM (n = 3, *P<0.5; n = 5, **P<0.01). Electron micrographs of human pericytes showed immuno-gold labeling for nerve/glial antigen 2 (NG2) (arrows) (e) but (f) no renin immuno-gold labeling. (g) Forskolin-treated pericyte showing immuno-gold labeling for human renin (arrows indicate the immuno-gold label). Renin-induced kidney pericyte primary cultures stained positively for pericyte markers: CD146, NG2, α-smooth muscle actin (αSMA) (all green), and renin (red). (h) Renin expression was not always correlated with αSMA expression (65.68 ± 7.4% overlap). Double positive cells for renin and αSMA are marked with arrowheads and renin+αSMA- cells are indicated by an asterisk. Renin is co-expressed unequivocally (100% overlap) with (i) CD146 and (j) NG2. Double positive cells for renin and pericyte markers are marked with arrowheads. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

From: Stefanska A, Kenyon C, Christian HC, Buckley C, Shaw I, Mullins JJ, Péault B.
Human kidney pericytes produce renin.
Kidney Int. 2016 Dec;90(6):1251-61.

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CD146 Antibody | OJ79c gallery image 10

Published customer image:
FITC conjugated Mouse anti Human CD146 antibody, clone oJ79c (MCA2141F) used to identify MCAM expression on circulating tumor cells in patients by immunofluorescence.
Image caption:
Representative fluorescence images of CTCs and a CTC cluster isolated from peripheral blood of patients with metastatic pancreatic cancer.
Enriched blood samples were stained with Hoechst 33343 (nuclei, blue), EpCAM (green), MCAM (green), and CD45 (red).

From: Lapin M, Tjensvoll K, Oltedal S, Buhl T, Gilje B, Smaaland R, Nordgård O.
MINDEC-An Enhanced Negative Depletion Strategy for Circulating Tumour Cell Enrichment.
Sci Rep. 2016 Jul 19;6:28929.

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CD146 Antibody | OJ79c gallery image 11

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Mouse anti Human CD146 antibody, clone OJ79c (MCA2141) used to identify canine pericytes in situ by immunofluorescence and isolated from adipose tissue by flow cytometry.
Image caption:
Canine PSC in situ identification.
Marker expression and isolation by fluorescence activated cell sorting (FACS). (A) Canine adipose tissue stained with pericytes marker CD146 (green), endothelial marker CD31 (red) (63×), and (B) adventitial cell marker, CD34 (red), CD146 (green) (40×). DAPI nuclear counterstain appears blue. (C) Cell size. (D) Purified PSC consist of distinct CD34-CD146+ pericytes and CD34+CD146- adventitial cells. (E) CD146+CD34- pericytes and (F) CD146-CD34+ adventitial cells express characteristic MSC markers, including CD44 and CD90. In comparison, (G) CD146-CD34- “non-PSC” are predominantly negative for CD44 and CD90. Scale bar: 25μM.

From: James AW, Zhang X, Crisan M, Hardy WR, Liang P, Meyers CA, et al. (2017)
Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.
PLoS ONE 12(5): e0177308.

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CD146 Antibody | OJ79c gallery image 12

Published customer image:
Mouse anti Human CD146 antibody, clone OJ79c (MCA2141) used to identify canine pericytes in situ by immunofluorescence staining of adipose cryosections.
Image caption:
Canine PSC in situ identification.
Marker expression (A) Canine adipose tissue stained with pericytes marker CD146 (green), endothelial marker CD31 (red) (63×), and (B) adventitial cell marker, CD34 (red), CD146 (green) (40×). DAPI nuclear counterstain appears blue. Scale bar: 25μM.

From: James AW, Zhang X, Crisan M, Hardy WR, Liang P, Meyers CA, et al. (2017)
Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.
PLoS ONE 12(5): e0177308..

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CD146 Antibody | OJ79c gallery image 13

Published customer image:
Mouse anti Human CD146 antibody, clone OJ79c (MCA2141) used to identify MCAM expressing circulating tumor cells by immunofluorescence.
Image caption:
Thumbnail gallery of analysed CTCs. Representative fluorescence images of a CTC (top row) and a CTC cluster (bottom row) isolated from the peripheral blood of a patient with pancreatic cancer. (Left column) Hoechst stain (blue) identifies nuclei; (second column) EpCAM-MCAM (green) identifies membranes of CTCs; (third column) CD45 (red) identifies leucocytes; (right column) superimposed images confirms intact cells. Images were acquired with 20× magnification. To enhance visibility, we adjusted the brightness and contrast equally for all microscopic images, and these adjustments were applied to the entire image

From: Lapin M, Tjensvoll K, Oltedal S, Javle M, Smaaland R, Gilje B, Nordgård O.
Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.
BMC Cancer. 2017 May 31;17(1):390.

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  • Mouse anti Human CD146:FITC
  • Mouse anti Human CD146:FITC
  • Mouse anti Human CD146
  • Mouse anti Human CD146:Alexa Fluor® 647
  • Mouse anti Human CD146:Alexa Fluor® 647
  • Mouse anti Human CD146
  • Mouse anti Human CD146:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OJ79c
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2141FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2141F
    MCA2141C, E, Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2141
    MCA2141XZC, E, Fdatasheet pdfdatasheet pdf1 mg
    MCA2141XZ
    MCA2141A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2141A647
    MCA2141FTFdatasheet pdfdatasheet pdf25 µg
    MCA2141FT
    MCA2141TC, E, Fdatasheet pdfdatasheet pdf25 µg
    MCA2141T
    MCA2141A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2141A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule (MCAM) or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmann et al. 1989).

      Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chen et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Dogyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      Pack Size: 0.2 mg0.09% Sodium Azide (NaN3)
      Pack Size: 25 µg
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      Pack Size: 0.2 mg0.09% Sodium Azide (NaN3)
      Pack Size: 25 µg
      0.09%Sodium Azide
      None present
    • Immunogen
      Recombinant human MUC18 (D1-D5) Fc protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      plasma membrane
      cell adhesion
      anatomical structure morphogenesis
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Melanoma
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Melanoma
    • Histology Positive Control Tissue
      Melanoma
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD146 Antibody Formats

    Formats Clone Applications Sizes available
    CD146 Antibody : Alexa Fluor® 647 OJ79c F 25 Tests/0.25ml | 100 Tests/1ml
    CD146 Antibody : Preservative Free OJ79c C, E, F 1 mg
    CD146 Antibody : Purified OJ79c C, E, F 25 µg | 0.2 mg
    CD146 Antibody : FITC OJ79c F 25 µg | 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Melanoma
              Melanoma
              Melanoma
              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

              Further Reading

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