CD126 Antibody | B-R6

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CD126 Antibody | B-R6 gallery image 1

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Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.
Image caption:
Circulating IL-6 and muscle IL-6 mRNA and IL-6 receptor response muscle lengthening contractions (MLC).(1a) Average serum interleukin-6 (IL-6) concentration. Time 0 hr corresponds to pre-intervention values; all other time-points correspond to post-intervention time (hr). (1b) Relative IL-6 mRNA expression, expressed as fold-change from 0 hr. (1c) Pearson correlation of serum IL-6 concentration versus muscle IL-6 mRNA (fold-change), correlation is representative of the individual data points and presented as mean values (●)±SD (error bars). (1d) Relative IL-6 receptor (IL-6Rα) mRNA expression, expressed as fold-change from 0 hr. (1e) Immunofluorescent image (40×) of a muscle cross-section triple-stained for Pax7 (red), IL-6Rα (green) and nuclei (DAPI?=?blue); IL-6Rα staining is apparent on the sarcolemma and satellite cell membrane. White arrow denotes one of the Pax7+ nuclei which co-localized with IL-6Rα (scale bar?=?100 μm). Values are reported as mean±S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p<0.05 vs. 0 hr.

From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. (2009)
Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.
PLoS ONE 4(6): e6027.

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CD126 Antibody | B-R6 gallery image 2

Published customer image:
Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.
Image caption:
Control Images: (S5a): Immunofluorescent (IF) stain of 7 µm muscle cross-sections for IL-6Rα (green - streptavidin-FITC), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6Rα and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6Rα antibody. (S5b): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for Pax7 (red - Alexa 594), nuclei (DAPI) and the secondary antibody used for IL-6 (secondary - tertiary only: immunoglobulin biotinylated secondary antibody + streptavidin-FITC) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibodies (FITC), or any FITC interaction with the Pax7 antibody. (S5c): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for IL-6 (green - streptavidin FITC), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6 and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6 antibody. (S5d): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for PCNA (red - Texas Red), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with PCNA and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody. (S5e): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for Pax7 (green - Alexa 488), nuclei (DAPI) and the secondary antibody used for PCNA (secondary only: Texas Red) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibody (Texas Red), or any Texas Red interaction with the Pax7 antibody. (S5f): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for phosphorylated STAT3 (p-STAT3) (red - Texas Red), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with p-STAT3 and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody.

From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. (2009)
Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.
PLoS ONE 4(6): e6027.

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  • Mouse anti Human CD126/Il-6R
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  • Product Type
    Monoclonal Antibody
  • Clone
    B-R6
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA822C, E, F, IF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA822
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD126 antibody, clone B-R6 recognizes human Interleukin-6 receptor subunit alpha, also known as CD126, IL-6R or Membrane glycoprotein 80. Human CD126 is a 468 amino acid single pass type I transmembrane glycoprotein bearing a single Ig-like C2 type domain, two fibronectin type III domains and a single WSXWS motif which may be involved in receptor avtivation (Daqil et al. 2012).

      Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Lu et al. 1995) and partially blocks binding of IL-6 to its receptor (Fourcin et al. 1994), clone B-R6 recognizes both the soluble and membranous human CD126

      Removal of Sodium azide is recommended prior to use in functional assays. The use of EQU003 for this purpose.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/50
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD126 Antibody Formats

    Formats Clone Applications Sizes available
    CD126 Antibody : Purified B-R6 C, E, F, IF, IP 0.1 mg
    • Copyright © 2016 Bio-Rad

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              • Application NameReference Images
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              References

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