CD120a Antibody | H398

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CD120a Antibody | H398 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti human CD120a: Alexa Fluor® 647 (MCA1340A647)

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  • CD120a Antibody | H398 thumbnail image 1
  • Mouse anti Human CD120a:RPE
  • Mouse anti Human CD120a:FITC
  • Mouse anti Human CD120a
  • Mouse anti Human CD120a:Alexa Fluor® 647
  • Mouse anti Human CD120a:Alexa Fluor® 647
  • Mouse anti Human CD120a:FITC
  • Mouse anti Human CD120a:RPE
  • Mouse anti Human CD120a:Preservative Free
  • Mouse anti Human CD120a
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  • Product Type
    Monoclonal Antibody
  • Clone
    H398
  • Isotype
    IgG2a
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1340FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1340F
    MCA1340E, F, Rdatasheet pdfdatasheet pdf0.2 mg
    MCA1340
    MCA1340XZE, F, FN, Rdatasheet pdfdatasheet pdf1 mg
    MCA1340XZ
    MCA1340PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1340PE
    MCA1340A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1340A647
    MCA1340FTFdatasheet pdfdatasheet pdf25 µg
    MCA1340FT
    MCA1340TE, F, Rdatasheet pdfdatasheet pdf25 µg
    MCA1340T
    MCA1340PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1340PET
    MCA1340A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1340A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD120a antibody, clone H398 recognizes an extracellular domain of the 55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75 kDa TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes.

      Mouse anti Human CD120a antibody, clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application.

      Mouse anti Human CD120a antibody, clone H398 inhibits the biological activity of both natural and recombinant human TNFalpha and TNFbeta (Thoma et al. 1990and Dri et al. 1999).

      Mouse anti Human CD120a antibody, clone H398 is routinely tested in flow cytometry on human peripheral blood monocytes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rabbityes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      Pack Size: 100 Tests
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      Pack Size: 25 Tests0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      Pack Size: 100 Tests
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      Pack Size: 25 Tests0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      None present
      0.09%Sodium Azide
    • Immunogen
      Purified human tumour necrosis factor receptor type 1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
    • Storage
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      membrane raft
      induction of apoptosis by extracellular signals
      tumor necrosis factor receptor activity
      apoptosis
      inflammatory response
      integral to plasma membrane
      extracellular region
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      positive regulation of transcription from RNA polymerase II promoter
      interspecies interaction between organisms
      positive regulation of inflammatory response
      prostaglandin metabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Radioimmunoassays
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Functional Assays
      Radioimmunoassays
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Radioimmunoassays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD120a Antibody Formats

    Formats Clone Applications Sizes available
    CD120a Antibody : RPE H398 F 25 Tests | 100 Tests
    CD120a Antibody : FITC H398 F 0.1 mg | 25 µg
    CD120a Antibody : Purified H398 E, F, R 0.2 mg | 25 µg
    CD120a Antibody : Preservative Free H398 E, F, FN, R 1 mg
    CD120a Antibody : Alexa Fluor® 647 H398 F 100 Tests/1ml | 25 Tests/0.25ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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        Useful Reagents

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          Human SeroblockBUF070B200 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              References

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