CD103 Antibody | 2G5.1

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  • Mouse anti Human CD103
  • Mouse anti Human CD103
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2G5.1
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA708C *, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA708
    MCA708TC *, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA708T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD103 antibody, clone 2G5.1 recognizes the human CD103 cell surface antigen, also known as the alphaE integrin, HML-1 antigen, Mucosal lymphocyte 1 antigen or Integrin alpha-E. CD103 is a 1161 amino acid, ~130 kDa single pass type 1 transmembrane glycoprotein.

      Mouse anti Human CD103 antibody, clone 2G5.1 reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from ascites
      Purified IgG prepared by affinity chromatography on Protein A from ascites
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.5%Bovine Serum Albumin
      0.09%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Human intestinal lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integrin-mediated signaling pathway
      receptor activity
      integrin complex
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/501/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/501/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Intestine
    • Histology Positive Control Tissue
      Intestine
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD103 Antibody Formats

    Formats Clone Applications Sizes available
    CD103 Antibody : Purified 2G5.1 C *, F, IP 25 µg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Intestine
              Intestine

              References

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