Mouse anti Human apolipoprotein H antibody, clone 1D2 recognises the human Apolipoprotein H molecule, also known as beta 2 glycoprotein 1, APC inhibitor, activated protein C-binding protein or anticardiolipin cofactor. Apolipoprotein H is expressed by placental trophoblast cells at high levels. Although the normal physiological role is not known, the protein appears to act as a co-factor for the binding of autoantibodies to phospholipids to trophoblasts, a process involved in the pathogenesis of recurrent miscarriage and thrombotic tendencies due to antiphospholipid syndrome.
Intended Use
Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Reconstitution
Preparation
Preservative Stabilisers
0.09%
Sodium Azide
Purity
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Test Principle
Buffer Solution
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name
Yes
No
Min Dilution
Max Dilution
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive control.
1. Mak, I.Y. et al. (2002) Regulated expression of signal transducer and activator of transcription, Stat5, and its enhancement of PRL expression in human endometrial stromal cells in vitro.J Clin Endocrinol Metab. 87 (6): 2581-8.
2. Vlachoyiannopoulos, P.G. et al. (2004) Anti-CD40 antibodies in antiphospholipid syndrome and systemic lupus erythematosus.Thromb Haemost. 92: 1303-11.
3. Lopez, D. et al. (2004) Oxidized low-density lipoprotein/beta2-glycoprotein I complexes and autoantibodies to oxLig-1/beta2-glycoprotein I in patients with systemic lupus erythematosus and antiphospholipid syndrome.Am J Clin Pathol. 121 (3): 426-36.
Further Reading
1. Chamley, L.W. et al. (2001) Is interleukin-3 important in antiphospholipid antibody-mediated pregnancy failure?Fertil Steril. 76 (4): 700-6.