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Alpha B Crystallin Antibody | 2E8

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Alpha B Crystallin Antibody | 2E8 gallery image 1

Western blotting analysis of anti alpha b crystallin antibody using Mouse anti Human alpha b crystallin clone 2E8 (MCA2697)
Lane 1: Recombinant alpha a crystallin
Lane 2: Recombinant alpha b crystallin

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  • Alpha B Crystallin Antibody | 2E8 thumbnail image 1
  • Mouse anti Human Alpha B Crystallin

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  • Product Type
    Monoclonal Antibody
  • Clone
    2E8
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2697E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2697
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human alpha B crystallin, clone 2E8 recognizes the basic (b) form of alpha crystallin, alpha B crystallin, a 20kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB5, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index.

      Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus. Studies have shown that a mutation in alpha B crystallin is responsible for desmin-related myopathy (DRM) and cataract (Perng et al. 1999.). Alpha B crystallin is also a major component of the astrocytic inclusions known as Rosenthal fibers (RFs), found in Alexander's disease (Iwaki et al. 1989).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from ascites
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Recombinant human alpha B crystallin (amino acids 1-175) purified from E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      GO:0005634
      nucleus
      Related reagents
      GO:0005212
      structural constituent of eye lens
      Related reagents
      GO:0006936
      muscle contraction
      Related reagents
      GO:0006916
      anti-apoptosis
      Related reagents
      GO:0009408
      response to heat
      Related reagents
      GO:0042803
      protein homodimerization activity
      Related reagents
      GO:0051260
      protein homooligomerization
      Related reagents
      GO:0006457
      protein folding
      Related reagents
      GO:0051082
      unfolded protein binding
      Related reagents
      GO:0032387
      negative regulation of intracellular transport
      Related reagents
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/10001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2697 detects a band of approximately 20kDa in mouse eye cell lysates.
    • Instructions For Use

    Additional Alpha B Crystallin Antibody Formats

    Formats Clone Applications Sizes available
    Alpha B Crystallin Antibody : Purified 2E8 E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              1. van Nierop, G.P. et al. (2015) Intrathecal CD8 T-cells of multiple sclerosis patients recognize lytic Epstein-Barr virus proteins.
              Mult Scler. pii: 1352458515588581.

              Further Reading

              1. Perng, M.D. et al. (1999) The cardiomyopathy and lens cataract mutation in alphaB-crystallin alters its protein structure, chaperone activity, and interaction with intermediate filaments in vitro.
              J Biol Chem. 274 (47): 33235-43.

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