Western blotting analysis of anti alpha b crystallin antibody using Mouse anti Human alpha b crystallin clone 2E8 (MCA2697) Lane 1: Recombinant alpha a crystallin Lane 2: Recombinant alpha b crystallin
Mouse anti Human alpha B crystallin, clone 2E8 recognizes the basic (b) form of alpha crystallin, alpha B crystallin, a 20kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB5, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index.
Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus. Studies have shown that a mutation in alpha B crystallin is responsible for desmin-related myopathy (DRM) and cataract (Perng et al. 1999.). Alpha B crystallin is also a major component of the astrocytic inclusions known as Rosenthal fibers (RFs), found in Alexander's disease (Iwaki et al. 1989).
Intended Use
Target Species
Human
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Reconstitution
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Recombinant human alpha B crystallin (amino acids 1-175) purified from E. coli.
Purity
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Test Principle
Buffer Solution
Phosphate buffered saline
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name
Yes
No
Min Dilution
Max Dilution
ELISA
Western Blotting
1/1000
1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended Protocol
ELISA
Immunohistology
Histology Positive Control Tissue
Immunofluorescence
Western Blotting
MCA2697 detects a band of approximately 20kDa in mouse eye cell lysates.
1. van Nierop, G.P. et al. (2015) Intrathecal CD8 T-cells of multiple sclerosis patients recognize lytic Epstein-Barr virus proteins.Mult Scler. pii: 1352458515588581.
Further Reading
1. Perng, M.D. et al. (1999) The cardiomyopathy and lens cataract mutation in alphaB-crystallin alters its protein structure, chaperone activity, and interaction with intermediate filaments in vitro.J Biol Chem. 274 (47): 33235-43.