ELISA Synblockis an ELISA buffer designed to avoid false positive results associated with buffers containing animal proteins (e.g. BSA) and reduce non-specific background signals in ELISA assays without the addition of the usuall protein additives.
- Intended Use
- ELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.
Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
- Product Form
- Ready to use - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.05% Sodium Azide
- Store at +4oC.
DO NOT FREEZE
- Shelf Life
- Please see label for expiry date.
- For research purposes only
Applications of ELISA Synblock
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Coat ELISA plate with antibody or antigen as required.
2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.
3. Add 300-400ul of BUF034A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.
4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|5x ELISA Coating Buffer||BUF030A||100 ml||E|
|10x ELISA Wash Buffer||BUF031A||100 ml||E|
Product Specific References
References for ELISA Synblock
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LeVine, H. et al. (2017) Down syndrome: Age-dependence of PiB binding in postmortem frontal cortex across the lifespan
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