ELISA Synblock

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ELISA Synblock

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF034A E 100 ml
BUF034B E 500 ml
BUF034C E 1000 ml
ELISA Synblockis an ELISA buffer designed to avoid false positive results associated with buffers containing animal proteins (e.g. BSA) and reduce non-specific background signals in ELISA assays without the addition of the usuall protein additives.

Product Details

Intended Use
ELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of ELISA Synblock

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF034A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
5x ELISA Coating Buffer BUF030A 100 ml E
10x ELISA Wash Buffer BUF031A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for ELISA Synblock

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