ELISA Ultrablock

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ELISA Ultrablock

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF033A E 100 ml
BUF033B E 500 ml
BUF033C E 1000 ml

Product Details

Intended Use
BUF033A is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.

Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted.
Shelf Life
Please see label for expiry date

More Information

Regulatory
For research purposes only

Applications of ELISA Ultrablock

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Instructions For Use
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF033A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
5x ELISA Coating Buffer BUF030A 100 ml E
10x ELISA Wash Buffer BUF031A 100 ml E
ELISA Ultrablock BUF033B 500 ml E
ELISA Ultrablock BUF033C 1000 ml E
ELISA Neptune Assay Diluent BUF039A 100 ml E
ELISA Ultrablock BUF033A 100 ml E

Product Specific References

References for ELISA Ultrablock

  1. Colwell, D.D. et al. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA.
    Vet Parasitol.174: 162-165.
  2. Defresne, F. et al. (2010) Differential influence of anticancer treatments and angiogenesis on the seric titer of autoantibody used as tumor and metastasis biomarker.
    Neoplasia 12: 562-570.
  3. Zhang, Y. et al. (2012) Causes of alternative pathway dysregulation in dense deposit disease.
    Clin J Am Soc Nephrol. 7 (2): 265-74.
  4. Krachudel, J. et al. (2013) Luteal insufficiency in bitches as a consequence of an autoimmune response against progesterone?
    Theriogenology. 79: 1278-83.