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ELISA Ultrablock

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ELISA Ultrablock thumbnail image 1 ELISA Ultrablock thumbnail image 2 ELISA Ultrablock thumbnail image 3
ELISA Ultrablock gallery image 1
Published customer image:
ELISA Ultrablock buffer(BUF033A) used as a blocking reagent in an ELISA for the evaluation of antibodies in murine serum samples recognizing β1AR-ECII.
Image caption:
Titer of antibodies against β1AR-ECII determined by ELISA throughout the study period, in sera of control (CTL, n = 10) and β1-immunized (AdrB1+, n = 7–10) mice. Data are represented as mean ±SEM, and normalized to basal optical density obtained before injection, *P ≤0.0.05; **P ≤0.0.01; ***P ≤0.0.001vs control.

From: Vanhoutte L, Guilbaud C, Martherus R, Bouzin C, Gallez B, Dessy C, Balligand JL, Moniotte S, Feron O.
MRI Assessment of Cardiomyopathy Induced by β1-Adrenoreceptor Autoantibodies and Protection Through β3-Adrenoreceptor Overexpression.
Sci Rep. 2017 Mar 9;7:43951.
doi: 10.1038/srep43951.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
ELISA Ultrablock gallery image 2
Published customer image:
ELISA UltraBlock buffer (BUF033A) used as a blocking reagent in an ELISA evaluating anti Factor H autoantibodies (FHAAs) in human serum samples.
Image caption:
FH autoantibodies (FHAAs) and key biomarkers at the age-of-onset of disease in patients with aHUS and C3G. Patient with M- proteins are in red. (A) Age; (B) FHAA titers in aHUS and C3G; (C) FHAAs (C3G and aHUS) with and without FHR1;

From: Zhang Y, Ghiringhelli Borsa N, Shao D, Dopler A, Jones MB, Meyer NC, Pitcher GR, Taylor AO, Nester CM, Schmidt CQ, Smith RJH.
Factor H Autoantibodies and Complement-Mediated Diseases.
Front Immunol. 2020 Dec 15;11:607211.
doi: 10.3389/fimmu.2020.607211.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
ELISA Ultrablock gallery image 3
Published customer image:
ELISA UltraBlock buffer (BUF033A) used as a blocking reagent in an ELISA evaluating levels of anti polymixin B antibodies in mouse plasma samples.
Image caption:
PMB-specific Ab responses in plasma.
A. The PMB-specific Ab titers were determined by ELISA. To establish the ELISA system, 1,000 ng/well of anti-PMB mAb and twofold serial dilutions were tested. Standard anti-PMB was titrated on PMB-coated plates with a sensitivity of 20 ng/well. B. Plasma samples from mice intranasally immunized with OVA plus 0, 5, 50, or 500 μg of PMB 3 times at weekly intervals were assessed by PMB-specific endpoint ELISA. Each dot represents an individual mouse. Horizontal and vertical bars indicate median and IQR, respectively, from 2 independent experiments (with 8 mice in each experimental group).

From: Yoshino N, Endo M, Kanno H, Matsukawa N, Tsutsumi R, Takeshita R, Sato S.
Polymyxins as novel and safe mucosal adjuvants to induce humoral immune responses in mice.
PLoS One. 2013 Apr 11;8(4):e61643.
doi: 10.1371/journal.pone.0061643.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

ELISA Ultrablock

Product Type
Accessory Reagent
Specificity
ELISA Ultrablock

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Product Code Applications Pack Size List Price Your Price Qty
BUF033C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml loader
List Price Your Price
loader
BUF033B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml loader
List Price Your Price
loader
BUF033A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml loader
List Price Your Price
loader

Intended Use
BUF033C
BUF033C is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.

Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
BUF033B
BUF033B is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.

Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
BUF033A
BUF033A is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.

Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Instructions For Use
BUF033C
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF033C and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
BUF033B
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF033B and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
BUF033A
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF033A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

Description Product Code Applications Pack Size List Price Your Price Quantity
5x ELISA Coating Buffer BUF030A E 100 ml
List Price Your Price
Description 5x ELISA Coating Buffer
10x ELISA Wash Buffer BUF031A E 100 ml
List Price Your Price
Description 10x ELISA Wash Buffer
ELISA Ultrablock BUF033A E 100 ml
List Price Your Price
Description ELISA Ultrablock
ELISA Ultrablock BUF033B E 500 ml
List Price Your Price
Description ELISA Ultrablock
ELISA Neptune Assay Diluent BUF039A E 100 ml
List Price Your Price
Description ELISA Neptune Assay Diluent
ELISA Ultrablock BUF033C E 1000 ml
List Price Your Price
Description ELISA Ultrablock

References for ELISA Ultrablock

  1. Colwell, D.D. et al. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA.
    Vet Parasitol.174: 162-165.
  2. Defresne, F. et al. (2010) Differential influence of anticancer treatments and angiogenesis on the seric titer of autoantibody used as tumor and metastasis biomarker.
    Neoplasia 12: 562-570.
  3. Zhang, Y. et al. (2012) Causes of alternative pathway dysregulation in dense deposit disease.
    Clin J Am Soc Nephrol. 7 (2): 265-74.
  4. Krachudel, J. et al. (2013) Luteal insufficiency in bitches as a consequence of an autoimmune response against progesterone?
    Theriogenology. 79: 1278-83.
  5. Yoshino, N. et al. (2013) Polymyxins as novel and safe mucosal adjuvants to induce humoral immune responses in mice.
    PLoS One. 8 (4): e61643.
  6. Liao, S. et al. (2016) Human placental growth hormone is increased in maternal serum at 20 weeks of gestation in pregnancies with large-for-gestational-age babies.
    Growth Factors. 34 (5-6): 203-9.
  7. Liao, S. et al. (2017) Maternal serum IGF-1, IGFBP-1 and 3, and placental growth hormone at 20weeks' gestation in pregnancies complicated by preeclampsia.
    Pregnancy Hypertens. 10: 149-54.
  8. Vanhoutte, L. et al. (2017) MRI Assessment of Cardiomyopathy Induced by β1-Adrenoreceptor Autoantibodies and Protection Through β3-Adrenoreceptor Overexpression.
    Sci Rep. 7: 43951.
    9. View The Latest Product References
  9. Ravindran, A. et al. (2018) C3 Glomerulopathy: Ten Years' Experience at Mayo Clinic.
    Mayo Clin Proc. 93 (8): 991-1008.
  10. Zhang, Y. et al. (2020) Factor H Autoantibodies and Complement-Mediated Diseases.
    Front Immunol. 11: 607211.
  11. Wang, Y. et al. (2021) Enhanced Bioactivity of a Human GHR Antagonist Generated by Solid-Phase Site-Specific PEGylation.
    Biomacromolecules. 22 (2): 299-308.
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