ELISA Ultrablock

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ELISA Ultrablock

Product Type: Accessory Reagent

Product Code	Applications	Pack Size
BUF033A	E	100 ml
BUF033B	E	500 ml
BUF033C	E	1000 ml

Product Details

Intended Use

BUF033A is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.

Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

Product Form

Ready to use - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.05%

Sodium Azide

Storage Information

Storage: Store at +4^oC.

DO NOT FREEZE

This product should be stored undiluted.
Guarantee: Guaranteed until date of expiry. Please see product label.

More Information

Regulatory: For research purposes only

Applications of ELISA Ultrablock

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Instructions For Use: 1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF033A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

Useful Reagents Available

Description	Product Code	Pack Size	Applications
5x ELISA Coating Buffer	BUF030A	100 ml	E
10x ELISA Wash Buffer	BUF031A	100 ml	E
ELISA Ultrablock	BUF033B	500 ml	E
ELISA Ultrablock	BUF033C	1000 ml	E
ELISA Neptune Assay Diluent	BUF039A	100 ml	E
ELISA Ultrablock	BUF033A	100 ml	E

Product Specific References

References for ELISA Ultrablock

Colwell, D.D. et al. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA.
Vet Parasitol.174: 162-165.
Defresne, F. et al. (2010) Differential influence of anticancer treatments and angiogenesis on the seric titer of autoantibody used as tumor and metastasis biomarker.
Neoplasia 12: 562-570.
Zhang, Y. et al. (2012) Causes of alternative pathway dysregulation in dense deposit disease.
Clin J Am Soc Nephrol. 7 (2): 265-74.
Krachudel, J. et al. (2013) Luteal insufficiency in bitches as a consequence of an autoimmune response against progesterone?
Theriogenology. 79: 1278-83.

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