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Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF032A E 100 ml
BUF032B E 500 ml
BUF032C E 1000 ml

Product Details

Intended Use
BUF032A is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide

Storage Information

Store at +4oC.


This product should be stored undiluted.
Guaranteed until date of expiry. Please see product label.

More Information

For research purposes only

Applications of ELISA BSA Block

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own systems using the appropriate negative/positive controls.
Instructions For Use
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF032A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
5x ELISA Coating Buffer BUF030A 100 ml E
10x ELISA Wash Buffer BUF031A 100 ml E
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