Browsing Modes for Business Users

ELISA BSA Block

ISO 9001 Quality Assurance Manufacturing | Print
ELISA BSA Block thumbnail image 1
ELISA BSA Block gallery image 1
A microtiter plate was coated over night with Mouse anti Bovine interferon-γ antibody, clone CC330 (MCA2112) at concentration of 5μg/ml in ELISA coating buffer (BUF030A) and recombinant bovine interferon gamma (PBP007) as a standard. After washing with ELISA Wash buffer (BUF031A) and blocking with ELISA BSA Block (BUF032A) samples were detected using biotinylated Mouse anti Bovine interferon-γ antibody (MCA1783B) at 5μg/ml followed by incubation with streptavidin:HRP conjugate (STAR5B) at 1/1000. Finally, TMB core HRP substrate (BUF056A) was applied for color development. Data is presented as the mean of three measurements.

ELISA BSA Block

Product Type
Accessory Reagent
Specificity
ELISA BSA Block

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
BUF032A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml loader
List Price Your Price
loader
BUF032B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml loader
List Price Your Price
loader
BUF032C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml loader
List Price Your Price
loader

Intended Use
BUF032A
BUF032A is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
BUF032B
BUF032B is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
BUF032C
BUF032C is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
BUF032A: <0.1% sodium azide (NaN3)
BUF032B: <0.1% sodium azide (NaN3)
BUF032C:
0.05%Sodium Azide
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own systems using the appropriate negative/positive controls.
Instructions For Use
BUF032A
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF032A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
BUF032B
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF032B and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
BUF032C
1. Coat ELISA plate with antibody or antigen as required.

2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

3. Add 300-400ul of BUF032C and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

Description Product Code Applications Pack Size List Price Your Price Quantity
5x ELISA Coating Buffer BUF030A E 100 ml
List Price Your Price
Description 5x ELISA Coating Buffer
10x ELISA Wash Buffer BUF031A E 100 ml
List Price Your Price
Description 10x ELISA Wash Buffer
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note