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Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
100 ml

500 ml

Product Details

Intended Use
BUF044A is a high performance p-nitrophenol phosphate (pNPP) solution, recommended for use in ELISA as a substrate for alkaline phosphatase (AP). In the presence of AP, pNPP is hydolyzed rapidly to p-nitrophenol and inorganic phosphate to produce a yellow solution.

This ready to use solution has been carefully formulated to increase pNPP activity and stability. BUF044A may be used for both kinetic and endpoint tests.
Product Form
Ready to use - liquid
Preservative Stabilisers
None present.

Storage Information

Store at +4oC. DO NOT FREEZE.
This product is photosensitive and should be protected from light.

Avoid exposure to light and heat

Store aliquots in bottles made of High Density Polyethylene (HDPE).
Guaranteed until date of expiry. Please see product label.

More Information

For research purposes only

Applications of pNPP

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Remove the pNPP substrate container from the refrigerator, and allow it to reach room temperature prior to use.

2. Add 100ul of BUF044A per microtiter well. Development time is typically 15-30 minutes. Optimum time is determined by the user and will vary according to the procedure and the incubation temperature.

3. For kinetic tests, measure absorbance at 405nm. For endpoint tests, add 100ul 1.0M sodium hydroxide solution to each well and mix throughly prior to measuring absorbance at 405nm /650nm.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra