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5x ELISA Coating Buffer

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A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in BUF030A. After washing with BUF031A and blocking with BUF032A samples were detected using MCA1783B antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate STAR5B at 1/1000. Finally, BUF056A was used as substrate. Data is presented as the mean of three measurements.

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5x ELISA Coating Buffer

Product Type
Accessory Reagent
Specificity
5x ELISA Coating Buffer

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Product Code Applications Pack Size List Price Your Price Qty
BUF030A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml
List Price Your Price
BUF030B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml
List Price Your Price
BUF030C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml
List Price Your Price

Intended Use
BUF030A is a proprietary coating buffer with pH 7.8 - 8.2 for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Product Form
5 x concentrate - liquid
Preservative Stabilisers
0.5%Sodium Azide
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Pack Size: 100 ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 1000 ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Instructions For Use
1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
1. Dilute the required volume of BUF030B 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
1. Dilute the required volume of BUF030C 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

Description Product Code Applications Pack Size List Price Your Price Quantity
10x ELISA Wash Buffer BUF031A E 100 ml
List Price Your Price
Description 10x ELISA Wash Buffer
ELISA BSA Block BUF032A E 100 ml
List Price Your Price
Description ELISA BSA Block

References for 5x ELISA Coating Buffer

  1. Böröcz, K. et al. (2022) Dynamic Features of Herd Immunity: Similarities in Age-Specific Anti-Measles Seroprevalence Data between Two Countries of Different Epidemiological History.
    J Clin Med. 11 (4): 1145.
  2. Hayden, Z. et al. (2021) Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders.
    J Immunol Res. 2021: 9953317.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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