5x ELISA Coating Buffer
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5x ELISA Coating Buffer
- Product Type
- Accessory Reagent
- Specificity
- 5x ELISA Coating Buffer
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- Intended Use
- BUF030A is a proprietary coating buffer with pH 7.8 - 8.2 for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage. - Product Form
- 5 x concentrate - liquid
- Preservative Stabilisers
0.5% Sodium Azide - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Pack Size: 100 ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 1000 ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
- Instructions For Use
- 1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation. - 1. Dilute the required volume of BUF030B 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation. - 1. Dilute the required volume of BUF030C 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| 10x ELISA Wash Buffer | BUF031A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | 10x ELISA Wash Buffer | ||||||
| ELISA BSA Block | BUF032A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | ELISA BSA Block | ||||||
References for 5x ELISA Coating Buffer
-
Böröcz, K. et al. (2022) Dynamic Features of Herd Immunity: Similarities in Age-Specific Anti-Measles Seroprevalence Data between Two Countries of Different Epidemiological History.
J Clin Med. 11 (4): 1145. -
Hayden, Z. et al. (2021) Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders.
J Immunol Res. 2021: 9953317.
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