5x ELISA Coating Buffer

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5x ELISA Coating Buffer

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF030A E 100 ml
BUF030B E 500 ml
BUF030C E 1000 ml

Product Details

Intended Use
BUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Product Form
5 x concentrate - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.5%Sodium Azide

Storage Information

Storage
Pack Size: 100 ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 1000 ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of 5x ELISA Coating Buffer

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Instructions For Use
1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
10x ELISA Wash Buffer BUF031A 100 ml E
ELISA BSA Block BUF032A 100 ml E