5x ELISA Coating Buffer

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Product Type: Accessory Reagent

Product Code	Applications	Pack Size
BUF030A	E	100 ml
BUF030B	E	500 ml
BUF030C	E	1000 ml

Summary
Useful Reagents

Product Details

Intended Use

BUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.

Product Form

5 x concentrate - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.5%	Sodium Azide

Storage Information

Storage: Pack Size: 100 ml
Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.

Pack Size: 500 ml, 1000 ml
Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Shelf Life: Please see label for expiry date.

More Information

Regulatory: For research purposes only

Applications of 5x ELISA Coating Buffer

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.

Instructions For Use: 1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
10x ELISA Wash Buffer	BUF031A	100 ml	E
ELISA BSA Block	BUF032A	100 ml	E

Request a different product with the 5x ELISA Coating Buffer specificity